Article Figures & Data
Figures
- FIG. 1.
Concentration of endogenous IL-10 in lung tissue after P. carinii (PC) challenge. (A) Control and CD4-depleted mice were inoculated with P. carinii, and lung tissue was assayed for IL-10 at serial intervals. An additional control included mice inoculated with PBS. Each data point represents mean ± SD for at least five animals. (B) Fixed lung tissue was also examined and scored microscopically for intensity of infection. Data represent the mean results for at least three animals.
- FIG. 2.
Expression of the vIL-10 transgene after intrapulmonary injection. A given amount (109 PFU) of AdvIL-10 was injected intratracheally (i.t.), and vIL-10 was assayed by ELISA in bronchoalveolar lavage fluid and in serum at serial intervals. Each data point represents the mean of three animals.
- FIG. 3.
Experimental design. Gene transfer of AdvIL-10 or AdLuc. i.t., intratracheal; H&E, hematoxylin and eosin; PC, P. carinii; and GMS, Gomori-methenamine silver; IP, intrapulmonary.
- FIG. 4.
Lung tissue burden of P. carinii (PC) in mice pretreated with AdvIL-10 or AdLuc. Data are from mice sacrificed 4 weeks after inoculation with P. carinii. The two leftmost columns represent control (CD4+) mice, and the two rightmost columns represent CD4-depleted (CD4−) mice. Each data point represents mean ± SD for at least 10 animals. it, intratracheal.
- FIG. 5.
Effect of pretreatment with AdvIL-10 or AdLuc on tissue inflammation. Data are from mice sacrificed 4 weeks after inoculation with P. carinii. The two leftmost columns represent control mice, while the two rightmost columns represent CD4-depleted mice. Alveolar inflammatory scores were calculated by microscopic examination of hematoxylin- and eosin-stained lung tissue. Each data point represents mean ± SD for at least six animals. *, P < 0.05 in comparison to AdLuc.
- FIG. 6.
Experimental design. Immune reconstitution during P. carinii (PC) infection. i.p., intrapulmonary; BALF, bronchoalveolar lavage fluid.
- FIG. 7.
Effect of AdvIL-10 or AdLuc on parameters of tissue inflammation during immune reconstitution. (A) Lung wet weights 2 weeks after immune reconstitution in mice treated with AdvIL-10 or AdLuc. *, P < 0.05 in comparison to AdLuc. (B) Concentrations of LDH in bronchoalveolar lavage fluid 2 weeks after immune reconstitution in mice treated with AdvIL-10 or AdLuc. *, P < 0.01 in comparison to AdLuc. (C) Lavaged neutrophils 2 weeks after immune reconstitution in mice treated with AdvIL-10 or AdLuc. *, P < 0.05 in comparison to AdLuc.
- FIG. 8.
Survival after immune reconstitution in mice treated with AdvIL-10 or AdLuc. Survival was significantly improved (P < 0.05) in mice receiving AdvIL-10 compared to that in mice receiving AdLuc.
