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- TABLE 1.
Experimental human bladder colonization with a mixture of wild-type E. coli 83972 and the papG deletion mutant E. coli HU2117
Subject % of wild-type E. coli 83972 in the urine samplea Inputb 1 week after instillation 4 weeks after instillation A 50 78 NAc B 50 0 0 C 50 18 12 D 50 54 98 E 50 56 58 E′ 50 4 0 F 50 100 100 G 50 88 98 H 50 100 100 I 50 NA 0 ↵ a Test subjects (A to I) were colonized with a mixed inoculum consisting of 50% each (106 total CFU) E. coli 83972 (papG+) and HU2117 (papGΔ) in a saline solution. Urine cultures were obtained at the intervals indicated. Fifty E. coli colonies from each culture were identified as either the wild-type E. coli 83972 strain or the papGΔ mutant. The titer of E. coli growing in subjects' urine varied between 105 and 106 per ml of urine.
↵ b Calculated value. Cultures of each strain were adjusted to the same optical density at 600 nm and mixed at a 50:50 ratio prior to bladder instillation. The approximate value was confirmed by using PCR in two experiments.
↵ c NA, subject not available for culture.
- TABLE 2.
Comparison of levels of human bladder colonization by E. coli HU2117 and E. coli HU2222
Subject % of E. coli HU2117 in the urine samplea Inputb 1 week after instillation 4 weeks after instillation 1 50 68 100 2 50 50 8 3 50 100 100 4 50 26 30 5 50 0 0 6 50 28 100 ↵ a Test subjects (1 to 6) were colonized with a mixed inoculum consisting of 50% each (106 total CFU) E. coli HU2117 (fimH+) and E. coli HU2222 (fimHΔ) in a saline solution. Urine cultures were obtained at the intervals indicated. Fifty E. coli colonies from each culture were identified as either the E. coli HU2117 strain or the fimHΔ mutant. The titer of E. coli growing in the urine of the subjects varied between 105 and 106 per ml of urine.
↵ b Calculated value. Cultures of each strain were adjusted to the same optical density at 600 nm and mixed at a 50:50 ratio prior to bladder instillation. The approximate value was confirmed by using PCR in two experiments.
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- Article
- ABSTRACT
- Construction of a deletion allele of the papG83972 gene and substitution of the deletion allele into the E. coli 83972 chromosome.
- Effect of the papG deletion upon bladder colonization.
- Construction of a deletion allele of the fimH83972 gene and substitution of the deletion allele into the E. coli 83972 chromosome.
- Effect of the fimH deletion upon bladder colonization.
- Conclusions.
- ACKNOWLEDGMENTS
- FOOTNOTES
- REFERENCES
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