Article Figures & Data
Figures
- FIG. 1.
Binding of anti-PC to A. actinomycetemcomitans and S. pneumoniae. Biotinylated affinity-purified human anti-PC was incubated with PC-containing A. actinomycetemcomitans D045D-40 (•) and S. pneumoniae 39937 (▪) in addition to PC-negative A. actinomycetemcomitans DB03A-42 (▾). It was then developed with Alexa 488-streptavidin. Binding, expressed as MCF, was determined by flow cytometry.
- FIG. 2.
Generation of oxidative burst in PMNs incubated with anti-PC-opsonized A. actinomycetemcomitans and S. pneumoniae. PC-containing A. actinomycetemcomitans D045D-40 (•) and S. pneumoniae 39937 (▪) in addition to PC-negative A. actinomycetemcomitans DB03A-42 (▾) were incubated with affinity-purified human anti-PC and then added to PMNs. Readings were made at 90-s intervals over a 45-min time course. The results are expressed as the MCF signal generated by opsonized bacteria minus the signal generated by unopsonized bacteria.
- FIG. 3.
Effect of anti-CD16 and anti-CD32 on the generation of an oxidative burst in PMNs incubated with anti-PC-opsonized A. actinomycetemcomitans and S. pneumoniae. PC-containing A. actinomycetemcomitans D045D-40 (•) and S. pneumoniae 39937 (▪) in addition to PC-negative A. actinomycetemcomitans DB03A-42 (▾) were incubated with affinity-purified human anti-PC and then added to PMNs. Readings were made at 90-s intervals over a 45-min time course. The results are expressed as the MCF signal generated by opsonized bacteria minus the signal generated by unopsonized bacteria. (a) PMNs were preincubated with anti-CD16 and then incubated with opsonized and unopsonized bacteria. (b) PMNs were preincubated with anti-CD32 and then incubated with opsonized and unopsonized bacteria.
