Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About IAI
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Infection and Immunity
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About IAI
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Molecular Pathogenesis

Identification of Escherichia coli Genes That Are Specifically Expressed in a Murine Model of Septicemic Infection

Muhammad A. Khan, Richard E. Isaacson
Muhammad A. Khan
Department of Veterinary Pathobiology, University of Illinois, Urbana, Illinois 61802
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: khanX033@umn.edu
Richard E. Isaacson
Department of Veterinary Pathobiology, University of Illinois, Urbana, Illinois 61802
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/IAI.70.7.3404-3412.2002
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • FIG. 1.
    • Open in new tab
    • Download powerpoint
    FIG. 1.

    (a) RT-PCR was performed to detect the presence of cat mRNA in ivi strains i673, i675, i681, i682, i683, i684, i686, and i689 (lanes 1 to 8, respectively); RNA was isolated from infected mice livers. Lane 9, RNA isolated from the liver of a mouse challenged with the wild-type E. coli strain i484 carrying pKK232-8; lane 10, RNA from the liver of an uninfected mouse; lane M, molecular size marker (numbers indicate base pairs). (b) RT-PCR was performed with RNA from in vitro-grown E. coli strain i484 containing pKK232-8 (lane 1), RNA from in vitro-grown ivi clones (lane 2), and RNA from the livers of mice challenged with ivi clones i673, i681, and i689 (lanes 3, 5, and 7, respectively). In addition, the RNAs from the three ivi clones were treated with RNase prior to RT-PCR (lanes 4, 6, and 8). Lane M, molecular size marker.

Tables

  • Figures
  • TABLE 1.

    Bacterial strains and plasmids

    Strain or plasmidRelevant characteristic(s)Reference or source
    E. coli strains
        i484O25:H autoaglutinating; human isolateThis study
        i659Derivative of i484; SmrThis study
        i665 E. coli strain HB101 containing pKK232-8This study
        i668 E. coli i659 containing pKK232-8This study
        i673In vivo-induced cloneThis study
        i675In vivo-induced cloneThis study
        i681In vivo-induced cloneThis study
        i682In vivo-induced cloneThis study
        i683In vivo-induced cloneThis study
        i684In vivo-induced cloneThis study
        i686In vivo-induced cloneThis study
        i689In vivo-induced cloneThis study
        HB101Δ(gpt-proA)62 leuB6 thi-1 lacY1 recA rpsL20 (Strr) ara-14 galK2 xyl-5 mtl-1 supE44 Δ(mcrBC-hsdRMS-mrr) 3
        SM10 λpir thi-1 thr-1 leuB6 supE44 tonA21 lacY1 rec::RP4-2-Tc::Mu Kmr 28
    Plasmids
        pKK232-8Ampr Cms; ColE1 origin 4
        pGP704Ampr; R6K origin 28
  • TABLE 2.

    Primers used for site-directed mutagenesis

    CloneGene or ORFMutagenic primer
    Δivi814 bglG GCCGCAAAGCGGTAGATCTGGCAAGTTATGACGGAG
    Wild type bglG GCCGCAAAGCGGTAGAGGGCAAGTTATGACGGAG
    Δivi814 o761 GGATCAAGTTATCTAGAAAAAGGCGTGTTTTCAAGCCAGCAATAACGAAATAATTGCCG
    Wild type o761 GGATCAAGTTATCTGAAAAAGGCGTGTTTCTCGCCAGCAATAACGAAATAATTGCCG
    Δivi815 ycjF GCAAAAGCAATATTCAGCAAGGTACCAGCTTAAACAGGC
    Wild type ycjF GCAAAAGCAATATTCAGCAATACCAGCTTAAACAGGC
    Δivi816 clpB CCCGGATAAATTCACGATCGCGTTTAATAAAAGAATGGATCC
    Wild type clpB CCCGGATAAATTCACGTTCGCGAATAAAAGAATGGATCC
    Δivi817—aGCCGTAGCGATTTAAAGATGCAATAACGGCG
    Wild type—GCCGTAGCGATTTAGATGCAATAACGGCG
    Δivi818ORF VCATCACCAGGATGGCTTTAAAGCAATAAGAGTTGGCC
    Wild typeORF VCATCACCAGGATGGCTTTGAGCAATAAGAGTTGGCC
    Δivi819 aroA CAGTCTTATCAGTCCCCGGGTAGCTATTTGG
    Wild type aroA CAGTCTTATCAGTCTCCGGGTACTTATTTTGG
    • ↵ a —, no similarity to sequences in GenBank.

  • TABLE 3.

    List of E. coli genes identified to be in vivo induced

    ClassGene or ORFMap positionProduct or function
    Amino acid metabolism dadX 26.688Alanine racemase
    aroA 20.651Chorismate biosynthesis
    argD 75.155Acetylomithine aminotransferase
    metK 66.492 S-Adenosylmethionine synthetase
    RNA and protein biosynthesis, degradation, and modification me 24.582mRNA turnover, maturation of 5S RNA
    rrsB 89.76216S rRNA
    tufB 89.962Elongation factor Tu
    fdhA 80.994Selenocysteine synthesis
    msrA 95.687Peptide methionine sulfoxide reductase
    hslV 88.794Heat shock protein VU, proteasome-related peptidease subunit
    clpA 19.885ATP-binding component of serine protease
    prpB 7.499Phosphoprotein phosphatase
    DNA replication, repair, restriction, and modification dinD 82.242DNA damage-inducible protein
    dinG 17.940Probably ATP-dependent helicase
    parE 68.363DNA topoisomerase IV subunit B
    uvrD 86.126DNA-dependent ATPase I and helicase II
    uvrB 17.519DNA repair; excision nuclease subunit B.
    dnaA 83.634DNA biosynthesis, initiation of chromosome replication, can be transcription regulator
    recG 82.403DNA helicase, resolution of Holliday junction, branch migration
    IS91197.134New member of the IS3 group of insertion sequences
    trs5 5.891Transposon-related function
    Cell division ftsX 77.570Cell division membrane protein
    Carbohydrate metabolism pgm 15.364Phosphoglucomutase
    bgl 83.8β-Glucoside metabolism
    malQ 76.427Maltose degrading enzyme/amylomaltase
    Surface structure csgA 23.79Fibronectin and Congo red binding curli pili of E. coli
    Peptidoglycan biosynthesis and metabolizing enzymes slt 99.764Soluble lytic murein transglycosylase
    bacA 68.998Bactracin resistance, possibly phosphorylates undecaprenol
    Transport system ugpB 77.354 sn-Glycerol 3-phosphate transport system, periplasmic binding protein
    fepE 13.310Ferric enterobactin (enterochelin) transport
    iucA Aerobactin biosynthesis
    dcuA 94.047Membrane transport of aspartase
    trkH 86.884Potassium uptake.
    gltS 82.451Sodium/glutamate symport carrier protein
    Outer membrane proteins nlpA 82.704Lipoprotein-28
    pldA 86.275Outer membrane phospholipase A
    Anaerobic respiration narW 33.08Appears to be essintial for nitrate reductase activity, probably by promoting the correct association of the alpha and beta subunits
    hybE 67.648Energy metabolism, carbon: anaerobic respiration.
    Drug or analog sensitivity Macromolecule biosynthesis and degradation tehA 32.303Tellurite resistance
    menG 88.731Menaquinone biosynthesis protein
    menA 88.743Menaquinone biosynthesis protein
    birA 89.9Biotin-[acetyl-coenzyme A-carboxylase] ligase
    fadB 86.79Fatty acid oxidation pathway
    tesB 10.207Fatty acid and phosphatidic acid biosynthesis
    gloA 37.202Methylglyoxal metabolism
    pntB 36.062Beta subunit of pyridine nucleotide transhydrogenase
    appA 22.414Phosphoanhydride phosphorylase
    mog 0.200Required for efficient incorporation of molybdate into molybdoproteins
    udk 46.136Uridine kinase
    Energy metabolism aceE 2.651Pyruvate dehydrogenase
    glxB2 11.575Glyoxylate-induced protein
    acn 28.752Aconitate hydratase
    Cellular repressor of the SOS response dinR Cellular repressor of the SOS response in Bacillus subtilis
    Unclassified yejF 49.024Putative ATP-binding component of a transport system
    yehU 47.658Putative 2 component sensor protein
    hslU 88.765Putative heat shock protein HslVU, ATPase-subunit, homologous to chaperones
    yihK 87.428Putative GTP-binding factor
    b1284 28.919Putative DEOR-type transcriptional regulator
    o169 9.57Putative regulator of flagellar biosynthesis, transcription initiation factor?
    o808 35.70Putative oxidoreductase
    f344 39.40Putative DNA helicase II
    o196 95.651Putative transport protein
    o275 8.32Probable ABC transporter permease protein
    f278 21.41Probable ABC transporter permease protein
    o251 41.83Hypothetical ABC transporter
    o127 90.77Putative arginine biosynthesis
    f216 23.749Putative regulator
    hycF 61.265Probable iron-sulfur protein of hydrogenase 3
    b1806 40.7Putative outer membrane protein
    yhjE 79.16Putative transport protein
    f290 87.667Putative aldose-1-epimerase
    Unknown function ygaF 60.095Hypothetical protein
    pfs 3.846Hypothetical protein
    yddE 33.912Hypothetical protein
    yfhH 58.123Hypothetical protein
    ycaJ 20.202Hypothetical protein encoded in serS 5′ region
    f178 38.94Hypothetical protein
    375 87.043Hypothetical RNA methyltransferase
    o425 87.551Hypothetical protein
    ycjF 29.823Hypothetical protein
    o423 78.000Hypothetical protein
    b2434 54.96Hypothetical protein
    b2362 53.3Hypothetical protein
    b1839 41.4Hypothetical protein
    o416 85.66Hypothetical protein encoded in rffE-rffT intergenic region
  • TABLE 4.

    In vivo-induced E. coli genes submitted to GenBank

    CloneAccession no.
    ivi931 AF249904
    ivi932 AZ877598
    ivi933 AZ877599
    ivi934 AF249901
    ivi935 AZ877597
    ivi936 AZ877600
    ivi937 AF249903
    ivi938 AF249902
    ivi939 AZ877601
    ivi940 AZ877602
  • TABLE 5.

    Comparisons of mutant and wild-type growth in the mouse competition model of septicemia

    MutantGene or ORFFunctionCompetition index (CFU of wild type/ CFU of mutant)
    Δivi813 bglG β-Glucoside-metabolizing enzyme2.1a
    Δivi814 o761 Unknown1.8a
    Δivi815 ycjF Hypothetical protein81.4a
    Δivi816 clpB ATP-dependent protease subunit2.7
    Δivi817No significant similarity to sequences in GenBank28.5a
    Δivi818ORF VSimilar to serine/threonine protein phosphatase7.6a
    Δivi819 aroA Aromatic amino acid biosynthesis2.7
    • ↵ a Significant (P > 0.05) based on Wilcoxon signed rank test.

PreviousNext
Back to top
Download PDF
Citation Tools
Identification of Escherichia coli Genes That Are Specifically Expressed in a Murine Model of Septicemic Infection
Muhammad A. Khan, Richard E. Isaacson
Infection and Immunity Jul 2002, 70 (7) 3404-3412; DOI: 10.1128/IAI.70.7.3404-3412.2002

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Infection and Immunity article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Identification of Escherichia coli Genes That Are Specifically Expressed in a Murine Model of Septicemic Infection
(Your Name) has forwarded a page to you from Infection and Immunity
(Your Name) thought you would be interested in this article in Infection and Immunity.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Identification of Escherichia coli Genes That Are Specifically Expressed in a Murine Model of Septicemic Infection
Muhammad A. Khan, Richard E. Isaacson
Infection and Immunity Jul 2002, 70 (7) 3404-3412; DOI: 10.1128/IAI.70.7.3404-3412.2002
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

Escherichia coli Infections
gene expression
Genes, Bacterial
sepsis

Related Articles

Cited By...

About

  • About IAI
  • Editor in Chief
  • Editorial Board
  • Policies
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Ethics
  • Contact Us

Follow #IAIjournal

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0019-9567; Online ISSN: 1098-5522