Article Figures & Data
Figures
- FIG. 1.
Analysis of the expression of mce1 genes. (A) Schematic representation of the mce operons. The arrowheads indicate the position of the junction primers (J1 to J5). (B) Amplicons of RT-PCR with junction primers. Primer sets used are indicated above each lane. M, 100-bp ladder as a size marker; -ve, negative control, where mce1 primers were used in a PCR without an RT step. The RNA concentration was 300 ng per 20 μl of reaction mixture. The fast-moving band has the primer dimers.
- FIG. 2.
Analysis of expression profile of mce operons in exponential phase. Amplicons of RT-PCR with specific primers for mce1, mce2, mce3, and mce4 and RNA from 10-day-old cultures as the template were analyzed by electrophoresis; the operon-specific primers used are indicated above the lanes. M, 100-bp ladder. Equal concentrations of RNA (500 ng per 20 μl of reaction mixture) were used in all experiments; rpoB was used as a positive control. The fast-moving band has the primer dimers.
- FIG. 3.
Analysis of expression profile of mce operons in stationary phase. Amplicons of RT-PCR with operon-specific primers as indicated above each lane were analyzed; rpoB was used as a positive control. M, 100-bp ladder. The RNA from 20-day-old cultures was used at a concentration of 500 ng per 20 μl of reaction mixture. The fast-moving band has the primer dimers.
- FIG. 4.
Analysis of expression profile of mce operons in bacilli from spleens of infected guinea pigs (A) and from pulmonary tubercles from infected rabbits (B). The amplicons are from RT-PCR with operon-specific primers as indicated above each lane; rpoB-specific primers were used as a positive control. M, 100-bp ladder; -ve, negative control, where the RT step was omitted for rpoB-specific primers to rule out DNA contamination. The RNA concentration was 300 ng per 20 μl of reaction mixture in each case. The fast-moving band has the primer dimers.
- FIG. 5.
Analysis of expression profile at exponential growth phase (A) and stationary growth phase (B), analyzed by RT-PCR with gene-specific primers for 10 genes reported to be expressed in infected macrophages. The gene amplified is indicated above each lane. M, 100-bp marker. The RNA concentration was 300 ng per 20 μl of reaction mixture in each case. mce1 was used as a positive control (A). The fast-moving band has the primer dimers.
- FIG. 6.
Schematic representation of the organization of mce2 and mce1, -3, and -4. Each open rectangle indicates a gene within the operon. Hatched rectangle, Rv0590A; filled rectangle, Rv0586 (encoding a putative lactate dehydrogenase regulator); arrow, putative start site of transcription of the first gene of the mce operon.
Tables
- TABLE 1.
Sequences of primers used in analysis of expression profile of mce operons and genes expressed during infection (5)
Gene or operon Forward primer Reverse primer mce2 CGACATGGCTTTCACCTCTG CCGACCCCACATCAATCAC mce3 CAACACCCGCGAGATTCAG GTTCTTCGAATGCAGTACC mce4 CACCTTCCTCATCCCCTC GATGAGCGATTGGAACAAC sigE CAATATCACGACCATCACGACCTT AGCACCACCGCGGCACGAAACT sigH GGCCTGGCTCTACCGGATACTGAC GGCTAAAAGACCGCGCACTGAC icl TGGGAGCAGCTGCACGACCTCG TGGGTCGGGATCAACACCTTC ponA1 GGGCGGCGGAGAAGGCGGTTGC ACGAAATGCGGCGGGTGGTAGATA uvrA CCGGCCGGGAAAGCATTGAGATAC CGTCGACCAGGGTGCGTAGATAGC pks2 CCGCACCCAGCCGACGCAAAAGAT GCCGCCGACGAAAACAAGCAGAAC ctpV CGGGTTTCGGCGGTGTTTGTCC CGGCCGCGCTCTTCCTGCTCCAC Rv3070 GTATTGGGTCCGTGTTGCGTTTTC CCATCTGGCGGTCCTCTCC Rv3843c TACGCCGGAGGACAGCAACTACG TACGGCGGCGCATCGAACAACTCC prrA GCTGGAACGCGGCTTACG CAGGTCGAATTCGCGCTTGGTCAG
