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Molecular Pathogenesis

Characterization of the Novel Factor Paa Involved in the Early Steps of the Adhesion Mechanism of Attaching and Effacing Escherichia coli

Isabelle Batisson, Marie-Pierre Guimond, Francis Girard, Hongyan An, Chengru Zhu, Eric Oswald, John M. Fairbrother, Mario Jacques, Josée Harel
Isabelle Batisson
1Laboratoire de Biologie des Protistes, UMR 6023, Université Blaise Pascal, Aubière
2Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec J2S 7C6, Canada
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Marie-Pierre Guimond
2Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec J2S 7C6, Canada
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Francis Girard
2Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec J2S 7C6, Canada
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Hongyan An
3Advantage International USA, Inc., Westport, Connecticut 06880
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Chengru Zhu
4Center for Vaccine Development, Division of Geographic Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201
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Eric Oswald
5Unité INRA-ENVT de Microbiologie Moléculaire, École Vétérinaire de Toulouse, 31076 Toulouse Cedex, France
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John M. Fairbrother
2Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec J2S 7C6, Canada
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Mario Jacques
2Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec J2S 7C6, Canada
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Josée Harel
2Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec J2S 7C6, Canada
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  • For correspondence: josee.harel@umontreal.ca
DOI: 10.1128/IAI.71.8.4516-4525.2003
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ABSTRACT

Nonenterotoxigenic porcine Escherichia coli strains belonging to the serogroup O45 have been associated with postweaning diarrhea in swine and adhere to intestinal epithelial cells in a characteristic attaching and effacing (A/E) pattern. O45 porcine enteropathogenic E. coli (PEPEC) strain 86-1390 induces typical A/E lesions in a pig ileal explant model. Using TnphoA transposon insertion mutagenesis on strain 86-1390, we found a mutant that did not induce A/E lesions. The insertion was identified in a gene designated paa (porcine A/E-associated gene). Sequence analysis of paa revealed an open reading frame of 753 bp encoding a 27.6-kDa protein which displayed 100, 51.8, and 49% homology with Paa of enterohemorrhagic E. coli O157:H7 strains (EDL933 and Sakai), PEB3 of Campylobacter jejuni, and AcfC of Vibrio cholerae, respectively. Chromosomal localization studies indicated that the region containing paa was inserted between the yciD and yciE genes at about 28.3 min of the E. coli K-12 chromosome. The presence of paa and eae sequences in the porcine O45 strains is highly correlated with the A/E phenotype. However, the observation that three eae-positive but paa-negative PEPEC O45 strains were A/E negative provides further evidence for the importance of the paa gene in the A/E activity of O45 strains. As well, the complementation of the paa mutant restored the A/E activity of the 86-1390 strain, showing the involvement of Paa in PEPEC pathogenicity. These observations suggest that Paa contributes to the early stages of A/E E. coli virulence.

  • Copyright © 2003 American Society for Microbiology
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Characterization of the Novel Factor Paa Involved in the Early Steps of the Adhesion Mechanism of Attaching and Effacing Escherichia coli
Isabelle Batisson, Marie-Pierre Guimond, Francis Girard, Hongyan An, Chengru Zhu, Eric Oswald, John M. Fairbrother, Mario Jacques, Josée Harel
Infection and Immunity Jul 2003, 71 (8) 4516-4525; DOI: 10.1128/IAI.71.8.4516-4525.2003

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Characterization of the Novel Factor Paa Involved in the Early Steps of the Adhesion Mechanism of Attaching and Effacing Escherichia coli
Isabelle Batisson, Marie-Pierre Guimond, Francis Girard, Hongyan An, Chengru Zhu, Eric Oswald, John M. Fairbrother, Mario Jacques, Josée Harel
Infection and Immunity Jul 2003, 71 (8) 4516-4525; DOI: 10.1128/IAI.71.8.4516-4525.2003
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KEYWORDS

Adhesins, Escherichia coli
Bacterial Adhesion
Escherichia coli

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