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Fungal and Parasitic Infections

Both CD1d Antigen Presentation and Interleukin-12 Are Required To Activate Natural Killer T Cells during Trypanosoma cruzi Infection

Malcolm S. Duthie, Maria Kahn, Maria White, Raj P. Kapur, Stuart J. Kahn
Malcolm S. Duthie
1Infectious Disease Research Institute
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Maria Kahn
1Infectious Disease Research Institute
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Maria White
1Infectious Disease Research Institute
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Raj P. Kapur
2Department of Pathology, Children's Hospital and Regional Medical Center, Seattle, Washington
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Stuart J. Kahn
1Infectious Disease Research Institute
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  • For correspondence: skahn@idri.org
DOI: 10.1128/IAI.73.3.1890-1894.2005
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ABSTRACT

Mechanisms of natural killer T (NKT)-cell activation remain unclear. Here, we report that during Trypanosoma cruzi infection, interleukin-12 (IL-12) deficiency or anti-CD1d antibody treatment prevents normal activation. The required IL-12 arises independently of MyD88. The data support a model of normal NKT-cell activation that requires IL-12 and TCR stimulation.

Detection of liver NKT cells is reduced at day four of T. cruzi infection.

Natural killer T (NKT) cells help control infections by Trypanosoma cruzi and other pathogens (5, 9, 10, 13, 28). How NKT cells become activated during these infections remains unclear. Possibilities include T-cell receptor (TCR) stimulation by CD1d presentation of pathogen-derived or self-derived glycolipid antigens or non-TCR stimulation through other NKT-cell receptors, such as the interleukin-12 (IL-12) receptor (2, 4, 11, 18, 29). To explore possible mechanisms of NKT-cell activation during T. cruzi infection, we developed an assay based on NKT-cell activation-induced surface receptor down modulation (14, 31) and our previous observation that during T. cruzi infection NKT cells undergo TCR and NK1.1 down modulation. Initially, we inoculated 7- to 10-week-old wild-type mice (C57BL/6 mice; Charles River, Wilmington, Mass.) with 2 × 105 CL strain T. cruzi trypomastigotes (23, 33) and monitored alterations in liver mononuclear cell TCRs and NK1.1. We found on day four of the infection a reproducible decrease in the number of detectable liver NKT cells and in the intensity of NK1.1 staining (Fig. 1), indicating liver NKT-cell activation.

FIG. 1.
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FIG. 1.

IL-12 and CD1d are required for NKT-cell activation during T. cruzi infection. Liver mononuclear cells were prepared from mice that were either uninfected, infected 4 days previously (2 × 105 trypomastigotes), or inoculated 4 days previously with dead T. cruzi (2 × 105 trypomastigotes). Heating at 55°C for 5 min generated dead trypomastigotes. Mononuclear cell populations were prepared and incubated first with the anti-FcR antibody 2.4G2 and then with fluorescent-conjugated anti-NK1.1 (clone PK136) and an anti-TCRβ -chain (clone H57-597) (both from BD PharMingen). Representative flow cytometry plots from wild-type, IL-12−/−, MyD88−/−, anti-CD1d antibody (Ab)-treated and control antibody-treated mice are shown; the circled areas indicate the NKT cells, and the numbers within the plots indicate the percentages of NKT cells within the plots. Below each set of representative cytometry plots is listed the mean percentage of reduction in the numbers of NKT cells of that infected group compared to its uninfected group. These data were derived from wild-type mice (uninfected, n = 17; infected, n = 15), IL-12−/− mice (uninfected, n = 13; infected, n = 7), MyD88−/− mice (uninfected, n = 4; infected, n = 8), anti-CD1d antibody-treated mice (uninfected, n = 6; infected, n = 4), control antibody-treated mice (uninfected, n = 6; infected, n = 4), and wild-type mice (mock-inoculated, n = 2; dead trypomastigote inoculated, n = 4). P values were determined by using Student's t test. SEM, standard errors of the means.

During T. cruzi infection, IL-12, but not MyD88, is required for normal NKT-cell activation.

IL-12 and IL-18 can activate or contribute to NKT-cell activation (6, 11, 20, 21). During infections, these cytokines may arise as a consequence of TLR signaling. In addition, NKT cells express TLR, so it is possible that direct recognition of pathogen-associated molecular patterns leads to NKT-cell activation (25). We analyzed the liver NKT-cell populations of IL-12p40−/− (The Jackson Laboratory, Bar Harbor, Maine) and MyD88−/− mice. MyD88 is an adaptor molecule that is critical for IL-18 signaling and required for many TLR signaling pathways (1, 17, 27). While T. cruzi infection reduced the detectable NKT cells in wild-type and MyD88−/− mice, the number of NKT cells and the intensity of NK1.1 staining did not decline in IL-12−/− mice (Fig. 1). This observation indicates that during T. cruzi infection, IL-12 is required for normal NKT-cell stimulation and receptor down modulation. Furthermore, the data argue that IL-12 can be generated independently of MyD88-dependent TLR signaling. Accordingly, splenocytes placed in ex vivo culture from infected wild-type and infected MyD88−/− mice produced similar amounts of IL-12 (Fig. 2). These data are in agreement with a recent report that demonstrates that during T. cruzi infection, IL-12 is stimulated independently of MyD88 (8). Taken together, our data argue that during T. cruzi infection, IL-12 signals, but neither IL-18 nor MyD88-dependent TLR signals, are required for normal NKT-cell activation.

FIG. 2.
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FIG. 2.

During T. cruzi infection, IL-12 production occurs independently of MyD88 signaling. Wild-type and MyD88−/− mice were uninfected or infected with 2 × 105 trypomastigotes for 3 days. Splenocytes (5 × 106) were cultured for 48 h in each well of a 24-well plate (Corning, Inc., Corning, N.Y.) in 1 ml of RPMI 1640 supplemented with 5% heat-inactivated fetal calf serum and 50,000 U of penicillin-streptomycin. Supernatants were analyzed by using an IL-2 enzyme-linked immunosorbent assay (BD PharMingen). Results are shown as the means and standard deviations (uninfected, n = 1, infected, n = 3).

During T. cruzi infection, CD1d is required for protective NKT-cell activation.

We have previously demonstrated that CD1d gene-deficient (CD1d−/−) mice develop greater parasitemia and tissue inflammation than wild-type mice, but since these mice develop without NKT cells, they cannot be used to investigate how NKT cells are activated (9, 10, 26). To investigate if during the infection NKT cells were activated by CD1d antigen presentation, we treated C57BL/6 mice with a blocking anti-CD1d antibody (clone 20H2; American Type Culture Collection, Manassas, Va.) or a control antibody (rat immunoglobulin G; Sigma, St. Louis, Mo.) and then inoculated the mice with T. cruzi (24). On day 4 of the infection, the liver NKT cells of the control antibody-treated mice were decreased in number and in NK1.1 staining intensity, whereas those of the anti-CD1d antibody-treated mice were decreased neither in number nor in NK1.1 staining intensity (Fig. 1). These data argue that CD1d antigen presentation was required for NKT-cell activation and that treatment with anti-CD1d would block protective NKT-cell functions during T. cruzi infection (9, 10). As expected, anti-CD1d antibody-treated mice, compared to control antibody-treated mice, suffered increased infection-induced weight loss and earlier death following a lethal inoculum of T. cruzi (Fig. 3A and B). Furthermore, following a sublethal inoculum, anti-CD1d monoclonal antibody treatment caused increased tissue inflammation (Fig. 3C and D). Together, these data strongly argue that during T. cruzi infection, TCR stimulation by CD1d antigen presentation is required for normal NKT-cell activation and protective functions.

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FIG. 3.

CD1d is required for protection during T. cruzi infection. C57BL/6 mice were injected intraperitoneally with 200 μg of anti-CD1d antibody or rat immunoglobulin G. Antibodies were injected 1 day before infection, on the day of infection, and every second day thereafter until day 14 of infection. (A and B) Antibody-treated female C57BL/6 mice (five mice per group) were inoculated with 5 × 105T. cruzi trypomastigotes. (A) On the indicated days, each mouse was weighed, and the means and standard errors of the percentages of weight change compared to the preinfection weight for each mouse is displayed. (B) Survival of the mice is shown and was analyzed by using the log rank statistic of Kaplan-Meier survival analysis (SPSS, Inc., Chicago, Ill.). For weight change and survival of anti-CD1d antibody-treated mice compared to control antibody-treated mice, the P value was <0.05. (C) C57BL/6 mice were inoculated with 2 × 105T. cruzi trypomastigotes and BALB/c mice were inoculated with 1 × 105T. cruzi trypomastigotes. Skeletal muscles were collected from antibody-treated mice on day 28 of infection and assessed for inflammation. Investigators blinded to the experimental conditions obtained five random ×100 images (Eclipse E200 microscope and Coolpix 4500 camera; Nikon, Tokyo, Japan) of the left and right quadriceps (10 images per mouse). The images were overlaid with a grid of 49 evenly dispersed points, and the percentage of points intersected by nuclei was determined. The percentage of points intersected by nuclei of uninfected quadriceps images was subtracted. Three mice per group were analyzed to provide 30 scores per group for statistical analysis by Student's t test. Representative hematoxylin and eosin-stained sections are shown. (D) The muscle inflammatory scores are presented as the means and standard errors.

To identify a T. cruzi CD1d-restricted antigen, CD1d-transfected L cells were pulsed with various trypomastigote preparations (live trypomastigotes, dead trypomastigotes, trypomastigote-conditioned media, and trypomastigote lysate) or 100 ng of α-galactosylceramide (α-GalCer)/ml (as a positive control). After washing, the antigen-pulsed CD1d-transfected L cells (2 × 105) were incubated overnight with a mouse invariant NKT-cell hybridoma (DN32.D3; 5 × 104 cells) (7), and the culture supernatants were assayed for IL-2. α-GalCer stimulated IL-2 production, unlike all of the trypomastigote preparations, suggesting that T. cruzi trypomastigotes do not express a CD1d-restricted NKT-cell-specific antigen (data not shown). In support of these in vitro data, the inoculation of mice with dead trypomastigotes did not activate NKT cells in vivo (Fig. 1). Together, these data suggest that during T. cruzi infection, the NKT-cell antigens may be self antigens. Live trypomastigotes might damage host cells and tissues to generate increased amounts of these self antigens.

This study demonstrates that during T. cruzi infection, normal NKT-cell activation requires (i) live T. cruzi inoculation, (ii) IL-12, and (iii) CD1d antigen presentation. The data support a model of NKT-cell activation during infections that requires CD1d presentation of an endogenous self antigen and IL-12 (6). The self antigens might provide weak stimulation that is normally incapable of activating NKT cells without IL-12 (6). These data are consistent with the previous findings that IL-12 enhances NKT-cell activation following exposure to low doses of α-GalCer and facilitates NKT-cell activation by self antigens during Salmonella enterica serovar Typhimurium infection (6, 19, 30). IL-12 might cause the antigen-presenting cells to increase expression of costimulatory molecules and thereby facilitate NKT-cell activation (12, 15, 16, 32). Alternatively, analogous to NK cells, IL-12 might act directly on NKT cells by countering inhibitory signals and thus allow activation of the NKT cells (11, 22). In contrast to our data and these studies, a recent report demonstrates that NKT-cell activation during Leishmania donovani infection is triggered by parasite-derived glycolipid antigens independently of IL-12 (3). It remains unclear which antigens stimulate NKT cells during T. cruzi infection. A better understanding of how NKT cells become activated may guide therapies to alleviate infection-induced morbidity and mortality.

ACKNOWLEDGMENTS

We thank Randy Brutkiewicz (Indiana University, Indianapolis) for providing CD1d-transfected L cells and the mouse NKT-cell hybridoma DN32.D3; Charles Scanga and Alan Sher (National Institutes of Health, Bethesda, Md.) for providing MyD88−/− mice (with the permission of Shizou Akira, Osaka University, Osaka, Japan); Yasuhiko Koezuka, Kirin Brewery, Ltd., Gunma, Japan, for providing α-GalCer; and Karen Krause, Lori Lager, and Sally Norton at Children's Hospital and Regional Medical Center, Seattle, Washington, for advice and assistance with histological analyses.

This work was supported by National Institutes of Health grant AI49455.

FOOTNOTES

    • Received 15 September 2004.
    • Returned for modification 22 October 2004.
    • Accepted 11 November 2004.
  • Copyright © 2005 American Society for Microbiology

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Both CD1d Antigen Presentation and Interleukin-12 Are Required To Activate Natural Killer T Cells during Trypanosoma cruzi Infection
Malcolm S. Duthie, Maria Kahn, Maria White, Raj P. Kapur, Stuart J. Kahn
Infection and Immunity Feb 2005, 73 (3) 1890-1894; DOI: 10.1128/IAI.73.3.1890-1894.2005

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Both CD1d Antigen Presentation and Interleukin-12 Are Required To Activate Natural Killer T Cells during Trypanosoma cruzi Infection
Malcolm S. Duthie, Maria Kahn, Maria White, Raj P. Kapur, Stuart J. Kahn
Infection and Immunity Feb 2005, 73 (3) 1890-1894; DOI: 10.1128/IAI.73.3.1890-1894.2005
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    • ABSTRACT
    • Detection of liver NKT cells is reduced at day four of T. cruzi infection.
    • During T. cruzi infection, IL-12, but not MyD88, is required for normal NKT-cell activation.
    • During T. cruzi infection, CD1d is required for protective NKT-cell activation.
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
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KEYWORDS

antigen presentation
Antigens, CD1
Chagas disease
Interleukin-12
Killer Cells, Natural
Lymphocyte Activation
Trypanosoma cruzi

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