Article Figures & Data
Figures
- FIG. 1.
Schematic representation of the distribution of marker alleles in the 22 variants of T. parva chromosome 1 observed in St72. The 72-01 variant is that shown in row 1. Alphabetical codes at each locus have been assigned colors for illustrative purposes as follows: A, blue; B, red; C, yellow, D, green; E, magenta. Contiguous markers are seen to reassort in blocks, with individual blocks also showing evidence of recombination.
- FIG. 2.
Inbreeding in St72 and St96 with respect to the 72-01 genotype. Frequencies are provided for genotypes sharing different levels of identity with the clone.
- FIG. 3.
Cluster analysis of genotypes in St72 (A) and St96 (B) based on pairwise distance matrices generated by the LIAN 3.1 software. Major clusters are indicated, and the position of the 72-01 genotype is highlighted in boldface text.
- FIG. 4.
Marker analysis of whole genomic DNA prepared from four successive generations of the Marikebuni isolate of T. parva. Lane 1, IL3014; lane 2, St70; lane 3, St72; lane 4, St96; 5, clone 72-01.
Tables
- TABLE 1.
Genealogy of T. parva Marikebuni stabilates used in this study
Passage Stabilate R. appendiculatus colony 1 IL1581 ILRAD 2 IL2245 ILRAD 3 IL3014 ILRAD 4 CTVM St70 NVRC 5 CTVM St72 CTVM 6 CTVM St96 CTVM - TABLE 2.
Additional satellite loci employed in the study, with associated primer sequencesa
Satellite Chromosome Sequence Amplicon size (bp) Forward Reverse MS47 1 GTCACAAGGGAAATCATGTCACTC GAGCCTTGAGTAGGTCTAAATTTG 398 MS48 1 CTACTTCTGGATCAGGTGTGGTGG GATTGAGACGATCCCGGTAGTCCT 223 MS49 1 CACACCGAACTTTGATCTCAC CACAACGGAGATTCATCTAACA 463 MS50 2 AGTTTGTTGACCCAACCTTACC GACGCTGTATCGCATAACTCAC 391 MS51 3 CCACCAACTCACTTTTTATCC GGCAGAAACCCTAACAAAAC 161 MS52 3 GACTATAGAAGGAAGTGCTCCA ATAGGATTATCCGGTACTTGC 220 MS53 3 GGCGATGAAAATATCAGGT ACGCCAGCACTTTATTCA 208 MS54 3 GAGTCGTACATTTTCTCAAGG CTAGTCTCTCCCCTCAGAGTC 348 MS55 3 CGCCTCAATACTCCTAACAC TGGGCTTAGTCCAGTGTTAT 243 MS56 3 AACCCTCTCTTTCAACTCCTA GATCACGACTCAAGTAACTGC 173 MS57 4 CATGCACACCGTAGGTATATT AGGTACCACACACACACTTTC 118 MS58 4 CGTATCGAACACACAGTTACAC CCACACACAAATATCTACCACT 300 MS59 4 TCAGATTCCCCAGTATTTCC CAGAATCTCCAAGTCAATTCTC 111 ↵ a The amplicon sizes shown are those found in the T. parva Muguga clone 3308 (www.tigr.org ).
- TABLE 3.
Size-polymorphic loci examined in the study, with associated primer sequencesa
Gene Chromosome Sequence Amplicon size (bp) Forward Reverse TP01_1233 1 GCAAAACGTTTACTCTTGTGACTT GAAACCTTGGAAATAAAGCTAACG 1,182 TP01_0966 1 AGATTATTTCTTGGATGATGACGA AGGAGGTGGAGGTTGTATTAAAGT 1,573 TP03_0861 3 GTTATCACGATTCTCTTCCAAAGC AATCAAGGATTAAATGTGCAGGA 1044 TP04_0051 4 CCACTGGTTCTTCCGATGTAA GTTGTCCAGAACCATCAGCA 792 ↵ a The amplicon sizes shown are those found in the T. parva Muguga clone 3308 (www.tigr.org ). Gene nomenclature is as used on www.tigr.org .
- TABLE 4.
Linkage analysis of genotypes in two T. parva Marikebuni stabilates using LIAN 3.1 softwarea
Parameter St72 St96 Clones Genotypes Clones Genotypes Total no. 231 48 142 18 D 0.081 (0.005) 0.317 (0.017) 0.029 (0.004) 0.159 (0.017) IA 0.241 0.104 0.158 0.0963 ↵ a www.adenine.biz.fh-weihenstephan.de/lian/. D, mean genetic distance between loci; IA, standardized index of association. Standard errors are indicated in parentheses.
- TABLE 5.
Antigenic variants and their frequencies among 28 clones derived from St72 as detected by PCR analysis of four biallelic locia
Antigenic variant Chromosome 2 Chromosome 3 Frequency Tp5 Tp9 Tp1 Tp4 1 A A A A 0.14 2 A A A B 0.14 3 A B A A 0.04 4 A B A B 0.57 5 A B B B 0.04 6 B B B B 0.07 ↵ a Alleles are designated A or B. Genotype 72-01 corresponds to variant 4.
