Article Figures & Data
Figures
- FIG. 1.
Expression of Il-11 and Cxcl-14 by lung macrophages and fibroblasts isolated from TB-susceptible I/St mice and TB-resistant A/Sn mice. Normalized Il-11 and Cxcl-14 gene expression levels are shown as severalfold differences relative to Hprt gene expression. (A) Lung macrophages (gray bars) express higher levels of Il-11 than lung fibroblasts (hatched bars) from both I/St and A/Sn mice. (B) Lung fibroblasts (hatched bars) express higher levels of Cxcl-14 than macrophages (gray bars) from mice of both strains. In each experiment, syngeneic lung macrophages or lung fibroblasts extracted from several mice were pooled. Expression levels of Il-11 and Cxcl-14 were measured by quantitative RT-PCR. Results are expressed as means (±standard errors) of triplicate assays from one of three experiments with similar results.
Tables
- TABLE 1.
Partial list of genes differentially expressed in I/St and A/Sn macrophagesa
Gene Score (d) forb: Protein or genee Accession no. Gene name Control cellsc Infected cellsd Chemokine and cytokine genes Scyb14f 10.4 5.3 Cxcl14, macrophage inflammatory protein 2 gamma AW120786 96953_at Il-11f 9.9 3.4 Interleukin 11 U03421 92266_at Scyb13f 7.4 6.1 Cxcl13, B-cell homing chemokine AF030636 102025_at Ccr5 NS 2.4 Chemokine (C-C motif) receptor 5 AF022990 161968_f_at Scya11 3.6 NS Small chemokine (C-C motif) ligand 11 (eotaxin-1) U77462 92742_at Il-6f 3.5 NS Interleukin 6 X54542 102218_at Scyb2 MIP-2a 3.2 NS Cxcl-2, macrophage inflammatory protein 2 alpha X53798 101160_at Scyb1 MIP-2 2.8 NS Cxcl-1, macrophage inflammatory protein 2 L12030 95349_g_at Il-17 −5.6 NS Interleukin 17 U35108 99349_at Scyb10 −3.0 NS Cxcl-10, macrophage interferon-activated protein 10 (IP-10) M33266 93858_at Scyb9 −2.9 NS Cxcl-9, monokine induced by gamma interferon (Mig) M34815 101436_at Immune response genes Ifi205 7.7 3.0 Interferon-activated gene 205 M74123 94224_s_at Ifi202a 5.3 2.0 Interferon-activated gene 202A AV229143 94774_at Saa3f 5.0 3.5 Serum amyloid A 3 X03505 102712_at Gbp2 −4.8 −2.0 Guanylate nucleotide binding protein 2 AJ007970 104597_at Chi3l3 NS 3.9 Chitinase 3-like 3 M94584 92694_at Gbp1 −5.1 NS Guanylate nucleotide binding protein 1 M55544 95974_at Psmb9 −4.8 NS Proteosome (prosome macropain) subunit beta type 9 D44456 93085_at Ifit2 −4.4 NS Interferon-induced protein with tetratricopeptide repeats 2 U43085 103639_at Mx2 −4.2 NS Myxovirus (influenza virus) resistance 2 J03368 102699_at Gbp3 −3.4 NS Guanylate nucleotide binding protein 3 AW047476 103202_at Mx1 −3.4 NS Myxovirus (influenza virus) resistance 1 M21038 98417_at Ifi47 −3.2 NS Gamma interferon-inducible protein M63630 104750_at Cytoskeletal/extracellular matrix genes Mglap 3.9 2.4 Matrix gamma-carboxyglutamate (gla) protein D00613 93866_s_at Csrp1 NS 2.9 Cysteine-rich protein 1 D88793 92608_at Adam8 NS 2.7 A disintegrin and metalloprotease domain 8 X13335 103024_at Coro1a NS 2.1 Coronin actin binding protein 1A AW122039 96648_at Mmp8f NS −2.4 Matrix metalloproteinase 8 U96696 94769_at Cldn4 3.2 NS Claudin 4 AB000713 101410_at Receptor/cell surface genes Fpr1 5.2 2.3 Formyl peptide receptor 1 L22181 99387_at Tm7sf1 −6.7 −7.0 Transmembrane 7 superfamily member 1 AI060729 103017_at Marco −5.1 −2.1 Macrophage receptor with collagenous structure U18424 102974_at Cd22 −5.0 −3.0 CD22 antigen L02844 102939_s_at Il-7r −3.6 −5.5 Interleukin 7 receptor M29697 99030_at Il1rl1 NS −2.5 Interleukin 1 receptor-like 1 Y07519 98501_at Taa1 7.8 NS Tumor-associated antigen 1 U35836 94643_at Tnfrsf9 4.6 NS Tumor necrosis factor receptor superfamily member 9 AA798611 103509_at Bdkrb 3.6 NS Bradykinin receptor beta L26047 101748_at Raet1c 3.3 NS Retinoic acid early transcript gamma D64162 102649_s_at Pira1 3.1 NS Paired-immunoglobulin-like receptor A1 U96682 95784_at Ly6a −4.3 NS Lymphocyte antigen 6 complex locus A X04653 93078_at Itpr5 −3.2 NS Inositol 145-triphosphate receptor 5 AF031127 101441_i_at Ifngr −3.2 NS Gamma interferon receptor M28233 99334_at Csf2rb1 −3.0 NS Colony-stimulating factor 2 receptor beta 1 M34397 94748_g_at ↵ a Expression levels of genes in I/St macrophages are given relative to A/Sn macrophages, with negative numbers indicating that the gene is expressed at a higher level in A/Sn macrophages.
↵ b NS, not significant.
↵ c Relative interstrain difference in gene expression in uninfected macrophages.
↵ d Relative interstrain difference in gene expression in infected macrophages.
↵ e Protein name abbreviation is given in case it differs from the gene name.
↵ f Results for these genes were confirmed by quantitative real-time assay.
- TABLE 2.
Real-time reverse transcription-PCR confirmation of array data
Gene In vitro infection with M. tuberculosis Expression level for I/St vs A/Sn macrophages PCRa Microarrayb Il-11 − 8.9 5.0 + 7.6 3.7 Il-6 − 3.6 2.1 + 4.5 2.2 Mmp8 − −3.4c NSd + −5.4 −2.1 Cxcl14 − 11.1 4.2 + 9.6 3.5 Cxcl13 − 28.1 5.1 + 30.7 7.2 Saa3 − 9.3 2.5 + 12.3 2.3
HTML Page - index.htslp
Files in this Data Supplement:
- Supplemental file 1
-
List of genes differently expressed in I/St and A/Sn lung macrophages
MS Word document, 524K. - Supplemental file 2
-
List of primers and probes used for real-time RT-PCR
MS Word document, 45K.
- Supplemental file 1
-
List of genes differently expressed in I/St and A/Sn lung macrophages
