Sel1 Repeat Protein LpnE Is a Legionella pneumophila Virulence Determinant That Influences Vacuolar Trafficking

Bacterial and yeast strains, growth conditions, and plasmids.Bacterial and yeast strains and plasmids used in this study are listed in Table 1. Bacteria were cultured aerobically at 37°C. L. pneumophila strains were grown in ACES [N-(2-acetamido)-2-aminoethanesulfonic acid] buffered yeast extract broth or on buffered charcoal yeast extract (BCYE) plates (23), and unless otherwise stated, Escherichia coli strains were cultured in Luria-Bertani (LB) broth or agar. Antibiotics were added to media at the following final concentrations for L. pneumophila (or E. coli ): kanamycin, 25 μg/ml (100 μg/ml); chloramphenicol, 6 μg/ml (12.5 μg/ml); and ampicillin, 100 μg/ml (100 μg/ml). Saccharomyces cerevisiae was cultured in YPD (1% yeast extract, 2% peptone, and 2% dextrose) or synthetic dropout medium (Clontech, CA) lacking appropriate nutrients at 30°C.

DNA, PCR, and cloning techniques.Plasmid DNA was purified using a QIAprep spin miniprep kit (QIAGEN, Hilden, Germany), and bacterial genomic DNA was isolated as described previously (2). DNA-modifying enzymes were used according to the manufacturer's instructions (Promega, WI). PCR amplification was performed using 200 ng of template DNA and approximately 0.25 μg of each oligonucleotide. Oligonucleotide sequences and annealing conditions used are listed in Table 2. Plasmid constructs were confirmed by DNA sequencing using a PRISM ready reaction dye deoxy terminator cycle sequencing kit and a 3730 DNA analyzer (Applied Biosystems, CA). DNA sequences were assembled using Sequencher 3.1.1 (Gene Codes Corp., MI).

Coculture of Acanthamoeba castellanii and L. pneumophila. A. castellanii ATCC 50739 was grown in 75-mm² tissue culture flasks (Sarstedt, Leicestershire, United Kingdom) with PYG 712 medium at 20°C without agitation (10). Cultures of A. castellanii that were 72 h old were harvested and seeded into 24-well tissue culture trays (Sarstedt) at a density of 2.5 × 10⁵ cells/well in A.c. buffer (0.1% trisodium citrate, 0.4 mM CaCl₂, 2.5 mM KH₂PO₄, 4 mM MgSO₄, 2.5 mM Na₂HPO₄, 0.05 mM ferric pyrophosphate). Stationary-phase L. pneumophila strains, also resuspended in A.c. buffer, were added at a multiplicity of infection (MOI) of 0.01, and the coculture was incubated at 37°C with 5% CO₂. At specific time points, samples from duplicate wells for each strain were plated onto BCYE agar and assessed for replication of L. pneumophila. Results were expressed as mean log₁₀ CFU ± standard deviations for three independent experiments.

Pulmonary infection of A/J mice with L. pneumophila.To examine the comparative virulence of L. pneumophila 130b and the lpnE::km derivative within an established animal model, mixed infections of mutant and wild-type L. pneumophila were performed as described previously (45, 46). Briefly, 6- to 8-week-old female A/J mice (Jackson Laboratory, ME) were anesthetized and inoculated intratracheally with 10⁶ CFU of wild-type and mutant bacteria at a ratio of 1:1. Twenty-four and 72 h after inoculation, mice were sacrificed and their lung tissue isolated. Tissue was homogenized using a Pro 200 homogenizer (Pro Scientific Inc., CT), and complete host cell lysis was attained by incubation of the homogenized tissue in 0.1% saponin for 10 min at 37°C. Serial dilutions were plated onto both plain and antibiotic-selective BCYE to obtain the total number of viable bacteria and the number of lpnE mutant bacteria colonizing the lung tissue. Mixed infections were also performed with lpnE::km/(pMip:lpnE) and lpnE::km/(pMIP:lpnE_52-375) strains in competition with L. pneumophila 130b/(pMIP).

Transcomplementation of L. pneumophila 130b lpnE::km with truncated lpnE.Truncated forms of lpnE were amplified using the oligonucleotides listed in Table 2 and cloned into the XbaI/PstI sites of pMIP (42). These plasmids were introduced into L. pneumophila 130b lpnE::km via electroporation as described previously (21). Briefly, 50-ml logarithmic-phase cultures were harvested via centrifugation (10,000 × g, 4°C, 15 min) and washed with cold phosphate-buffered saline (PBS) and then cold 10% (vol/vol) glycerol before being resuspended in 10% glycerol with approximately 500 ng of plasmid DNA and subjected to electrophoresis at 2,300 V, 100 Ω, and 25 mF.

Tissue culture conditions and L. pneumophila invasion assays.The human monocytic cell line THP-1 and the human alveolar epithelial cell line A549 were propagated and prepared for infection as described previously (42). Stationary-phase strains of L. pneumophila were added at an MOI of 5 for THP-1 cells and an MOI of 100 for A549 cells and allowed to infect cells for 2 h in 5% CO₂ at 37°C. Cells were then treated with 100 μg/ml gentamicin for 1 h to kill extracellular bacteria and washed with PBS before being lysed with 0.01% digitonin. Serial dilutions of the inoculum and bacteria recovered from lysed cells were plated on BCYE agar, and results are expressed as percentages of the inoculum that resisted killing by gentamicin (means ± standard deviations for at least three independent experiments).

Avoidance of LAMP-1 by L. pneumophila-containing vacuoles.Immunofluorescence was used to investigate the ability of Legionella-containing vacuoles to avoid lysosomal fusion, using the lysosome-associated membrane protein LAMP-1 as a marker for lysosomal membranes, in both A549 and THP-1 cells. Immunofluorescence was performed as described previously (47). Briefly, 10⁵ A549 cells were seeded onto 12-mm glass coverslips (Menzel-Glaser, Braunschweig, Germany) and grown overnight before being infected with stationary-phase L. pneumophila at an MOI of 100. Alternatively, 10⁵ THP-1 cells were allowed to differentiate in the presence of phorbol 12-myristate 13-acetate for 72 h, ensuring differentiation into adherent, elongated cells, before being infected with stationary-phase L. pneumophila at an MOI of 5. Infection proceeded for 5 h or 24 h before cells were washed with PBS and fixed with 4% (wt/vol) paraformaldehyde (pH 7.4). Following fixation, the coverslips were blocked for 1 h with PBS containing 10% fetal bovine serum and then stained with monoclonal mouse anti-L. pneumophila (6026; ViroStat, ME) and, subsequently, anti-mouse immunoglobulin G (IgG)-Alexa Fluor 594 (Invitrogen, CA). Following labeling of the extracellular bacteria, cells were permeabilized for 1 h with PBS containing 10% fetal bovine serum and 0.05% saponin. Cells were washed with PBS and then stained with anti-L. pneumophila and rabbit anti-human LAMP-1 H-288 (Santa Cruz Biotechnology, CA). Anti-rabbit-Alexa Fluor 488 and anti-mouse-Alexa Fluor 594 or -Alexa Fluor 405 were used as secondary antibodies, and all antibodies were diluted in PBS with 10% fetal bovine serum, used at 1:50 (Legionella), 1:100 (LAMP-1), or 1:200 (secondary antibodies), and incubated with the cells for 1 h at 37°C. At the end of this staining procedure, intracellular bacteria appeared green, LAMP-1 appeared red, and extracellular bacteria appeared red or blue. Coverslips were mounted in DAKO fluorescent mounting medium (DAKO Corporation) and stored at 4°C in the dark. Slides were examined under a 100× objective on an Olympus BX51 microscope (Olympus, Tokyo, Japan). Images were acquired using an Olympus DP-70 digital camera and merged using DP controller software, version 1.1.1.71. LAMP-1 avoidance was scored blind for various strains of L. pneumophila, and data for at least 50 intracellular bacteria from three independent coverslips were expressed as mean percentages of LAMP-1 avoidance (± standard deviations). Differences in LAMP-1 avoidance were assessed for significance using an unpaired two-tailed t test.

Production of recombinant LpnE and polyclonal anti-LpnE antibodies.Full-length lpnE was amplified by PCR, using MBP-lpnE F and lpnE R (Table 2), and cloned into the XbaI/PstI sites of the pMAL-c2x expression vector (New England Biolabs, MA) to generate a maltose binding protein (MBP) fusion with LpnE. Production of this fusion protein was induced in E. coli BL21 with 1 mM isopropyl-β-d-thiogalactopyranoside (IPTG) and subsequently purified using amylose agarose as described by the manufacturer (Scientifix, Australia). Polyclonal antibodies were generated against column-purified MBP-LpnE resuspended in incomplete Freund's adjuvant by inoculation of 500 μg of protein into a rabbit on days 0, 28, and 49 before exsanguination on day 66 (Chemicon International, Inc., CA). Immune serum was adsorbed against L. pneumophila 130b lpnE::km and diluted 1:500 for immunoblotting in 0.05% Tween (vol/vol) in Tris-buffered saline.

TCA precipitation of culture supernatants. L. pneumophila extracellular proteins were precipitated from bacterial culture supernatants as described previously (38). Fifty-milliliter samples of stationary- or late-logarithmic-phase broth cultures of L. pneumophila were subjected to centrifugation (10,000 × g, 4°C, 15 min), and the supernatants were filtered through 0.22-μm filters (Millipore, MA). A 10% (wt/vol) final concentration of trichloroacetic acid (TCA) was added to precipitate the proteins, which were pelleted by centrifugation (10,000 × g, 4°C, 1 h) and washed with 100% (vol/vol) cold methanol before being dried and resuspended in 2× sodium dodecyl sulfate (SDS) sample buffer. TCA precipitation included the use of previously described lspDE and dotA mutants (47). TCA-precipitated culture supernatants and whole-cell lysate samples of strains of interest were separated by SDS-polyacrylamide gel electrophoresis and transferred to nitrocellulose for immunoblotting. Rabbit anti-E. coli RpoB antibodies (34) were used at 1:4,000 as a control for cytoplasmic protein contamination of supernatant samples, and rabbit anti-Lpg1905 (1:500) (47) was used to control for loading of TCA-precipitated proteins. When investigating the presence of truncated forms of LpnE in culture supernatants, Novex 4 to 20% acrylamide-Tris-glycine gels were utilized to ensure the recovery of small peptides.

Yeast two-hybrid system. lpnE was amplified with the lpnE-Y2HF and lpnE R oligonucleotides (Table 2) and cloned into pGBT9 digested with BamHI and PstI. The resulting plasmid was transformed into S. cerevisiae AH109 by the lithium acetate method as described previously (28). This strain was mated with S. cerevisiae Y187 pretransformed with a human HeLa Matchmaker GAL4 library according to the manufacturer's protocol (Clontech). Protein-protein interactions were selected by plating the mating mixture onto synthetic dropout medium plates lacking adenine, histidine, tryptophan, and leucine. Library plasmids were isolated from resulting yeast colonies and rescued in E. coli KC8 on M9 minimal medium containing the required nutrients, except for tryptophan. Library plasmids and pGBT9:lpnE were transformed into S. cerevisiae PJ69-4A, and β-galactosidase liquid culture assays (o-nitrophenyl-β-d-galactoside) were performed in triplicate according to the Clontech manual for the Matchmaker yeast two-hybrid system. The interaction between LpnE and specific regions of obscurin-like protein 1 (OBSL1) was examined in the same manner. A region of OBSL1 encompassing multiple immunoglobulin (Ig)-like domains and a second region encoding an Ig-like domain and a fibronectin (Fn) domain were cloned into pGAD424 digested with EcoRI and BamHI. The single Ig domain of the Fn fragment (Ig_Fn) was also cloned into the EcoRI/BamHI fragment of pGAD424. The resulting plasmids were introduced into S. cerevisiae PJ69-4A, and the yeast strains were assessed for β-galactosidase activity.

Contribution of lpnE to infection of A. castellanii and A/J mice.Previously, we reported that lpnE was required for efficient uptake of L. pneumophila into A549 epithelial cells and THP-1 macrophages (42). In this work, we extended our virulence studies to examine the contribution of lpnE to replication of L. pneumophila in A. castellanii and in the lungs of A/J mice. Coculture of L. pneumophila lpnE::km and A. castellanii demonstrated that lpnE was necessary for efficient infection of the amoeba (Fig. 1A). For up to 48 h postinfection, the lpnE mutant was recovered in significantly smaller numbers than wild-type L. pneumophila 130b. Bacterial numbers 72 h after infection were comparable between 130b and the lpnE mutant. The small reduction in CFU observed for the lpnE mutant during the first 48 h of A. castellanii infection was restored to wild-type levels upon transcomplementation of the lpnE mutant with full-length lpnE (Fig. 1A).

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FIG. 1.

(A) L. pneumophila infection of A. castellanii. Amoebae were infected at an MOI of 0.01 with wild-type L. pneumophila 130b (▪) or the lpnE::km (○) or lpnE::km/(pMIP:lpnE) (▴) mutant. Bacterial CFU were determined at 24, 48, and 72 h postinfection and are represented as mean log₁₀ CFU ± standard deviations for three independent experiments. *, significantly different from 130b (P = 0.034 at 24 h and P = 0.003 at 48 h [unpaired, two-tailed t test]). (B) Lung colonization of A/J mice by L. pneumophila 130b (▪) and an lpnE::km mutant (○). Strain 130b and the lpnE::km mutant were introduced into the lungs of A/J mice via intratracheal inoculation at a ratio of 1:1. Twenty-four and 72 h following infection, the numbers of CFU for 130b and the lpnE::km mutant were determined. Data are expressed as means ± standard deviations of the log₁₀ CFU recovered per lung (n = 7 or 8). *, significantly different from 130b at 72 h (P = 0.018; unpaired, two-tailed t test). (C) CIs of derivatives of L. pneumophila 130b in mixed infections with the wild-type parent strain. The pairs tested were as follows: lpnE::km mutant versus 130b (○), lpnE::km/(pMIP:lpnE) mutant versus 130b/(pMIP) (▴), and lpnE::km/(pMIP:lpnE_52-375) mutant versus 130b/(pMIP) (•). *, CI was significantly different from that for the lpnE::km mutant versus 130b (P < 0.05; unpaired, two-tailed t test).

The reduced ability of the lpnE mutant to infect macrophages, alveolar epithelial cells, and amoebae highlighted the importance of LpnE for host-pathogen interactions in both mammalian and protozoan hosts. The contribution of lpnE to infection of A/J mice was also examined by evaluating the ability of the lpnE mutant strain to compete with wild-type L. pneumophila 130b following intratracheal infection of A/J mice. Lung colonization was examined 24 and 72 h after infection (Fig. 1B). The mutant strain was recovered in similar numbers to wild-type L. pneumophila 130b 24 h after inoculation, but at 72 h significantly fewer mutant bacteria than L. pneumophila 130b bacteria were recovered. We also calculated the competitive index (CI) of the lpnE mutant by comparing the ratio of mutant to wild-type CFU in the lungs of mice at 72 h to the ratio of mutant to wild-type CFU in the inoculum. In general, a mutant with a CI of <0.5 is considered attenuated (5). At 72 h, the mean CI of the lpnE mutant was 0.22 ± 0.13 (Fig. 1C). Complementation of the lpnE mutant with full-length lpnE restored the CI of the mutant strain to 1.75 ± 1.58. These values were significantly different (P = 0.019; unpaired two-tailed t test), indicating that lpnE was needed for efficient colonization of the lungs of A/J mice (Fig. 1C).

Trafficking of the lpnE mutant in tissue culture cells.Although we showed previously that despite an initial invasion defect the lpnE mutant can replicate in THP-1 and A549 cells to wild-type levels (42), we did not examine the ability of the mutant to avoid trafficking to late endosomes and lysosomes. Here we investigated the capacity of the lpnE mutant vacuole to avoid fusion with late endosomes by determining the percentage of lpnE vacuoles that avoided the late endosomal marker LAMP-1 5 h after infection. We performed these experiments with A549 cells and THP-1 cells, with the latter undergoing extended treatment with phorbol 12-myristate 13-acetate to ensure differentiation into elongated cells, which are easier to view using immunofluorescence microscopy. We found that 5 h after infection, the majority of vacuoles containing a dotA mutant were positive for LAMP-1 in both A549 and THP-1 cells (Fig. 2 and 3). In contrast, the majority of L. pneumophila 130b vacuoles avoided LAMP-1 (Fig. 2 and 3). Interestingly, the lpnE mutant exhibited an intermediate trafficking phenotype. The percentage of lpnE mutant vacuoles that avoided LAMP-1 was significantly different from those observed for both the wild-type and the dotA mutant in A549 cells and for the wild-type in THP-1 cells (Fig. 3). This indicated that the inactivation of lpnE altered vacuole trafficking in tissue culture cells. Complementation of the lpnE mutant with full-length lpnE restored the ability of L. pneumophila to evade fusion with LAMP-1 (Fig. 3). Interestingly, the percentage of LAMP-1 avoidance for wild-type L. pneumophila was notably lower for THP-1 cells than for A549 cells 5 h after infection. This suggested that THP-1 cells had a greater capacity to drive endosome fusion, which may relate to their monocytic lineage and/or the extended differentiation time used to produce flat cells for microscopy. Since the lpnE mutant showed decreased avoidance of LAMP-1, we also examined the ability of the lpnE mutant to form vacuoles containing multiple (>10) bacteria. Similar to wild-type L. pneumophila, the lpnE mutant was still able to form vacuoles containing multiple bacteria, and these did not associate with LAMP-1 (Fig. 2B).

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FIG. 2.

(A) Immunofluorescence of LAMP-1 in A549 cells infected for 5 h with wild-type L. pneumophila 130b or the dotA::cm, lpnE::km, or lpnE::km/(pMIP:lpnE) mutant. LAMP-1 was detected with an anti-LAMP-1 mouse monoclonal antibody diluted 1/100 followed by the secondary antibody, anti-mouse-Alexa Fluor 488, diluted 1/200. Bacteria were detected with anti-lipopolysaccharide (anti-LPS) antibodies raised in rabbits and diluted 1/50, followed by the secondary antibody Alexa Fluor 594 diluted 1/200. (B) Immunofluorescence of LAMP-1 in A549 cells infected for 24 h with wild-type L. pneumophila 130b or the dotA::cm, lpnE::km, or lpnE::km/(pMIP:lpnE) mutant. Primary and secondary antibodies were the same as for panel A.

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FIG. 3.

Percentage of Legionella-containing vacuoles that avoided LAMP-1 after infection of A549 cells (A) and THP-1 cells (B) for 5 h. P values of <0.05 (unpaired two-tailed t test) are indicated. LAMP-1 avoidance was scored blind according to the staining patterns indicated in Fig. 4A.

Transcomplementation of the lpnE mutant with truncated forms of lpnE.To investigate the putative role of the eight SLR regions of LpnE in host-pathogen interactions, four truncated versions of lpnE were designed to encompass the N terminus (amino acids 1 to 51), the N terminus plus two SLR domains (amino acids 1 to 122), the N terminus and the first six SLR domains (amino acids 1 to 266), and the eight SLR domains plus the C terminus (amino acids 52 to 375) (Fig. 4A). These lpnE truncations were cloned into the pMIP complementation vector, which utilizes the constitutively active mip promoter region (56), and introduced into the lpnE mutant. The ability of these derivatives of L. pneumophila to establish infection of both THP-1 macrophages and A549 alveolar epithelial cells was investigated (Fig. 4B and C). As reported previously, disruption of lpnE significantly reduced the ability of L. pneumophila to infect both cell lines, which was restored upon transcomplementation with full-length lpnE (Fig. 4B and C) (42). In contrast, transcomplementation of the mutant strain with truncated forms of lpnE did not restore wild-type levels of invasion. The introduction of lpnE_1-51, lpnE_1-122, or lpnE_1-266 had no influence on the compromised ability of the lpnE mutant to infect THP-1 or A549 cells. However, lpnE_52-375, encoding all of the SLR regions of LpnE but lacking the N-terminal 51 amino acids, was able to partially complement the lpnE mutant defect in both cell types (Fig. 4B and C). We also examined the ability of the lpnE mutant complemented with lpnE_52-375 to colonize the lungs of A/J mice in mixed infections with wild-type L. pneumophila. The CI of the lpnE::km strain carrying pMIP:lpnE_52-375 in competition with wild-type L. pneumophila/(pMIP) at 72 h was 0.59 ± 0.11, indicating partial complementation of the virulence defect (Fig. 1C).

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FIG. 4.

(A) Schematic representation of L. pneumophila SLR protein LpnE and truncated variants created for this study. Shaded rectangles represent the SLR regions, and black rectangles signify the predicted N-terminal 22-amino-acid signal peptide. The truncations were used to complement L. pneumophila lpnE::km, and resulting strains were examined for uptake by THP-1 macrophages (B) and A549 epithelial cells (C). Data are expressed as percentages of the amount of inoculum that was intracellular following a 2-h infection and 1-h gentamicin treatment and are means ± standard deviations for at least three independent experiments. *, significantly different from the lpnE::km mutant (P < 0.05; unpaired two-tailed t test).

Presence of LpnE in culture supernatants.The reduced invasion phenotype of the lpnE mutant in THP-1 and A549 cells and the replication defect of the mutant in A. castellanii and A/J mice suggested that LpnE may play a direct role in host-pathogen interaction. To determine the localization of LpnE in L. pneumophila, rabbit polyclonal antibodies were raised against purified MBP-LpnE and used to detect LpnE within stationary-phase L. pneumophila cultures. Immunoblot analysis showed that LpnE was present in the culture supernatant of L. pneumophila 130b (Fig. 5A), indicating that the protein was extracellular. Interestingly, the presence of LpnE in culture supernatants appeared to be independent of the virulence-related Lsp type II secretion system (46) and also independent of the Dot/Icm type IV secretion system, as LpnE was found in culture supernatants derived from both lspDE and dotA mutant strains of L. pneumophila 130b (Fig. 5A). To confirm that the presence of LpnE in the culture supernatant was not due to cell breakdown and contamination with cytoplasmic proteins, the same samples were incubated with anti-RpoB to detect a known cytoplasmic protein. In addition, we used antibodies to the secreted protein Lpg1905 (47) to control for protein loading (Fig. 5A). Apart from a lack of LpnE expression in the lpnE mutant and overexpression of LpnE from the complementing pMIP plasmid, LpnE was present in equivalent amounts in all other strains tested, including wild-type L. pneumophila 130b and the secretion system mutants (Fig. 5A).

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FIG. 5.

Immunoblot analysis of culture supernatants precipitated with TCA and detected with anti-LpnE, anti-Lpg1905, and anti-RpoB antibodies. (A) Stationary-phase TCA-precipitated culture supernatants and whole-cell lysate samples from derivatives of L. pneumophila 130b were separated by SDS-polyacrylamide gel electrophoresis. Lane 1, L. pneumophila 130b; lane 2, lpnE::km mutant; lane 3, lpnE::km/(pMIP:lpnE) mutant; lane 4, ΔdotA mutant; lane 5, ΔlspDE mutant. (B) TCA-precipitated stationary-phase culture supernatants. Lane 1, L. pneumophila lpnE::km/(pMIP:lpnE) mutant; lane 2, lpnE::km/(pMIP:lpnE_1-51) mutant; lane 3, lpnE::km/(pMIP:lpnE_1-122) mutant; lane 4, lpnE::km/(pMIP:lpnE_1-266) mutant; lane 5, lpnE::km/(pMIP:lpnE_52-375) mutant. The presence of both LpnE_1-266 and LpnE_52-375 in the culture supernatant is indicated by arrows and the predicted molecular size of each protein.

We also examined export of the truncated forms of LpnE. The predicted products of lpnE_1-51 and lpnE_1-122 were not detected with anti-LpnE, even in whole-cell lysate samples (data not shown). This indicated that these small truncations of LpnE were either not recognized by our anti-LpnE sera or, alternatively, that lpnE_1-51 and lpnE_1-122 were not expressed in vitro. However, both LpnE_1-266 and LpnE_52-375 were expressed and present in culture supernatants (Fig. 5B). This demonstrated that neither the C terminus, absent in LpnE_1-266, nor the predicted N-terminal signal sequence, absent in LpnE_52-375, was essential for LpnE export. At this stage, the mechanism by which LpnE reaches the culture supernatant is unknown.

LpnE interacts with Ig-like domains of eukaryotic proteins via the SLR regions.The presence of LpnE in culture supernatants provided further evidence that during infection, the protein may interact with host proteins to stimulate the uptake of L. pneumophila. Since TPR regions in general are known to mediate protein-protein interactions, the ability of LpnE to bind to eukaryotic proteins was examined using the yeast two-hybrid system. Screening of full-length LpnE against a HeLa cell cDNA library identified several putative binding partners of LpnE (Table 3). Recovery of the interacting clones revealed that the most prevalent binding partner, accounting for 28.6% of rescued clones, was OBSL1. This eukaryotic protein, ranging in molecular mass from 130 to 230 kDa, is a member of the Unc-89/obscurin gene family, exhibiting homology to the N-terminal region of the giant muscle protein obscurin, which is known to interact with titin, small ankyrin 1, and myosin within vertebrate skeletal muscle to mediate muscle contraction (3, 27, 57). The function of OBSL1 is unknown, although, similar to obscurin, the protein possesses a number of putative Ig-like domains and an Fn domain (Fig. 6A). While other putative eukaryotic binding partners identified here may warrant further investigation, particularly the epsilon subunit of COPI involved in eukaryotic endosome trafficking and phagosome maturation (6), we initially examined the interaction between LpnE and OBSL1 as the most frequently recovered target. The specificity of the interaction between OBSL1 and LpnE was confirmed and quantified within S. cerevisiae PJ69-4a by using liquid culture β-galactosidase assays (Fig. 6B). The truncated forms of LpnE were also tested for the ability to bind to OBSL1. LpnE_1-51 and LpnE_1-122 could not bind OBSL1, but LpnE_1-266 and LpnE_52-375, with six and eight SLR regions, respectively, were able to interact with OBSL1. Curiously, LpnE_1-266 exhibited the strongest interaction with OBSL1, but full-length LpnE and LpnE_52-375 showed an equivalent strength of interaction with OBSL1, demonstrating that the full repertoire of SLR domains is needed for wild-type-strength protein-protein interaction (Fig. 6B). To investigate the basis of the interaction of LpnE with OBSL1 in more detail, two sections of OSBL1, one comprising the Fn type 3 domain and an Ig domain (Fn) and a second comprising the C-terminal Ig domains of OBSL1 (Ig) (Fig. 6A), were cloned into pGAD424 and examined for the ability to interact with LpnE (Fig. 6B). Both fragments of OBSL1 exhibited strong binding to LpnE, suggesting that the protein-protein interaction occurred through the Ig-like domains common to both fragments. In an attempt to confirm that LpnE interacted specifically with Ig-like domains, we cloned the 8.3-kDa predicted Ig_Fn domain into pGAD424 and examined its interaction with LpnE. β-Galactosidase assays for S. cerevisiae carrying pGBT9:LpnE and pGAD424:Ig_Fn demonstrated no interaction between the Ig_Fn peptide and LpnE. Although this lack of interaction may indicate that the Ig domains do not mediate an interaction between OBSL1 and LpnE, it is more likely that such a small peptide is unable to establish the tertiary structure required for protein-protein interactions.

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FIG. 6.

(A) Domain organization of OBSL1, comprising several Ig-like domains (circles) and one Fn domain (diamond). The regions of OBSL1 subcloned for protein-protein interaction studies, namely, Ig, Fn, and Ig_Fn, are indicated. (B) Protein-protein interactions were assessed using the yeast two-hybrid system β-galactosidase reporter assay. Full-length LpnE, LpnE_1-266, and LpnE_52-375 showed strong interactions with OBSL1, as did full-length LpnE with two of the truncated forms of OBSL1 but not with Ig_Fn.