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Molecular Pathogenesis

ace, Which Encodes an Adhesin in Enterococcus faecalis, Is Regulated by Ers and Is Involved in Virulence

Francois Lebreton, Eliette Riboulet-Bisson, Pascale Serror, Maurizio Sanguinetti, Brunella Posteraro, Riccardo Torelli, Axel Hartke, Yanick Auffray, Jean-Christophe Giard
Francois Lebreton
1Laboratoire de Microbiologie de l'Environnement, EA 956 soutenue par l'INRA, IFR 146, Université de Caen, 14032 Caen Cedex, France
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Eliette Riboulet-Bisson
1Laboratoire de Microbiologie de l'Environnement, EA 956 soutenue par l'INRA, IFR 146, Université de Caen, 14032 Caen Cedex, France
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Pascale Serror
2Unité des Bactéries Lactiques et Pathogènes Opportunistes, INRA, Domaine de Vilvert, 78350 Jouy-en-Josas, France
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Maurizio Sanguinetti
3Institute of Microbiology, Catholic University of Sacred Heart, L. go F. Vito 1, 00168 Rome, Italy
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Brunella Posteraro
3Institute of Microbiology, Catholic University of Sacred Heart, L. go F. Vito 1, 00168 Rome, Italy
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Riccardo Torelli
3Institute of Microbiology, Catholic University of Sacred Heart, L. go F. Vito 1, 00168 Rome, Italy
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Axel Hartke
1Laboratoire de Microbiologie de l'Environnement, EA 956 soutenue par l'INRA, IFR 146, Université de Caen, 14032 Caen Cedex, France
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Yanick Auffray
1Laboratoire de Microbiologie de l'Environnement, EA 956 soutenue par l'INRA, IFR 146, Université de Caen, 14032 Caen Cedex, France
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Jean-Christophe Giard
1Laboratoire de Microbiologie de l'Environnement, EA 956 soutenue par l'INRA, IFR 146, Université de Caen, 14032 Caen Cedex, France
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  • For correspondence: jean-christophe.giard@unicaen.fr
DOI: 10.1128/IAI.01218-08
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ABSTRACT

Enterococcus faecalis is an opportunistic pathogen that causes numerous infectious diseases in humans and is a major agent of nosocomial infections. In this work, we showed that the recently identified transcriptional regulator Ers (PrfA like), known to be involved in the cellular metabolism and the virulence of E. faecalis, acts as a repressor of ace, which encodes a collagen-binding protein. We characterized the promoter region of ace, and transcriptional analysis by reverse transcription-quantitative PCR and mobility shift protein-DNA binding assays revealed that Ers directly regulates the expression of ace. Transcription of ace appeared to be induced by the presence of bile salts, probably via the deregulation of ers. Moreover, with an ace deletion mutant and the complemented strain and by using an insect (Galleria mellonella) virulence model, as well as in vivo-in vitro murine macrophage models, we demonstrated for the first time that Ace can be considered a virulence factor for E. faecalis. Furthermore, animal experiments revealed that Ace is also involved in urinary tract infection by E. faecalis.

  • Copyright © 2009 American Society for Microbiology
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ace, Which Encodes an Adhesin in Enterococcus faecalis, Is Regulated by Ers and Is Involved in Virulence
Francois Lebreton, Eliette Riboulet-Bisson, Pascale Serror, Maurizio Sanguinetti, Brunella Posteraro, Riccardo Torelli, Axel Hartke, Yanick Auffray, Jean-Christophe Giard
Infection and Immunity Jun 2009, 77 (7) 2832-2839; DOI: 10.1128/IAI.01218-08

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ace, Which Encodes an Adhesin in Enterococcus faecalis, Is Regulated by Ers and Is Involved in Virulence
Francois Lebreton, Eliette Riboulet-Bisson, Pascale Serror, Maurizio Sanguinetti, Brunella Posteraro, Riccardo Torelli, Axel Hartke, Yanick Auffray, Jean-Christophe Giard
Infection and Immunity Jun 2009, 77 (7) 2832-2839; DOI: 10.1128/IAI.01218-08
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KEYWORDS

Bacterial Proteins
Carrier Proteins
Enterococcus faecalis
Gene Expression Regulation, Bacterial
Repressor Proteins
transcription factors

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