Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About IAI
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Infection and Immunity
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About IAI
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Bacterial Infections

Sequence Analysis and Characterization of a Transferable Hybrid Plasmid Encoding Multidrug Resistance and Enabling Zoonotic Potential for Extraintestinal Escherichia coli

Timothy J. Johnson, Dianna Jordan, Subhashinie Kariyawasam, Adam L. Stell, Nathan P. Bell, Yvonne M. Wannemuehler, Claudia Fernández Alarcón, Ganwu Li, Kelly A. Tivendale, Catherine M. Logue, Lisa K. Nolan
Timothy J. Johnson
1Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, Minnesota 55108
2Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, Iowa 50011
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dianna Jordan
2Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, Iowa 50011
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Subhashinie Kariyawasam
2Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, Iowa 50011
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Adam L. Stell
1Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, Minnesota 55108
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nathan P. Bell
1Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, Minnesota 55108
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yvonne M. Wannemuehler
2Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, Iowa 50011
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Claudia Fernández Alarcón
1Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, Minnesota 55108
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ganwu Li
2Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, Iowa 50011
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kelly A. Tivendale
2Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, Iowa 50011
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Catherine M. Logue
3Department of Veterinary and Microbiological Sciences, North Dakota State University, Fargo, North Dakota 58105
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lisa K. Nolan
2Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, Iowa 50011
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: lknolan@iastate.edu
DOI: 10.1128/IAI.01174-09
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

ABSTRACT

ColV plasmids of extraintestinal pathogenic Escherichia coli (ExPEC) encode a variety of fitness and virulence factors and have long been associated with septicemia and avian colibacillosis. These plasmids are found significantly more often in ExPEC, including ExPEC associated with human neonatal meningitis and avian colibacillosis, than in commensal E. coli. Here we describe pAPEC-O103-ColBM, a hybrid RepFIIA/FIB plasmid harboring components of the ColV pathogenicity island and a multidrug resistance (MDR)-encoding island. This plasmid is mobilizable and confers the ability to cause septicemia in chickens, the ability to cause bacteremia resulting in meningitis in the rat model of human disease, and the ability to resist the killing effects of multiple antimicrobial agents and human serum. The results of a sequence analysis of this and other ColV plasmids supported previous findings which indicated that these plasmid types arose from a RepFIIA/FIB plasmid backbone on multiple occasions. Comparisons of pAPEC-O103-ColBM with other sequenced ColV and ColBM plasmids indicated that there is a core repertoire of virulence genes that might contribute to the ability of some ExPEC strains to cause high-level bacteremia and meningitis in a rat model. Examination of a neonatal meningitis E. coli (NMEC) population revealed that approximately 58% of the isolates examined harbored ColV-type plasmids and that 26% of these plasmids had genetic contents similar to that of pAPEC-O103-ColBM. The linkage of the ability to confer MDR and the ability contribute to multiple forms of human and animal disease on a single plasmid presents further challenges for preventing and treating ExPEC infections.

  • Copyright © 2010 American Society for Microbiology
View Full Text
PreviousNext
Back to top
Download PDF
Citation Tools
Sequence Analysis and Characterization of a Transferable Hybrid Plasmid Encoding Multidrug Resistance and Enabling Zoonotic Potential for Extraintestinal Escherichia coli
Timothy J. Johnson, Dianna Jordan, Subhashinie Kariyawasam, Adam L. Stell, Nathan P. Bell, Yvonne M. Wannemuehler, Claudia Fernández Alarcón, Ganwu Li, Kelly A. Tivendale, Catherine M. Logue, Lisa K. Nolan
Infection and Immunity Apr 2010, 78 (5) 1931-1942; DOI: 10.1128/IAI.01174-09

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Infection and Immunity article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Sequence Analysis and Characterization of a Transferable Hybrid Plasmid Encoding Multidrug Resistance and Enabling Zoonotic Potential for Extraintestinal Escherichia coli
(Your Name) has forwarded a page to you from Infection and Immunity
(Your Name) thought you would be interested in this article in Infection and Immunity.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Sequence Analysis and Characterization of a Transferable Hybrid Plasmid Encoding Multidrug Resistance and Enabling Zoonotic Potential for Extraintestinal Escherichia coli
Timothy J. Johnson, Dianna Jordan, Subhashinie Kariyawasam, Adam L. Stell, Nathan P. Bell, Yvonne M. Wannemuehler, Claudia Fernández Alarcón, Ganwu Li, Kelly A. Tivendale, Catherine M. Logue, Lisa K. Nolan
Infection and Immunity Apr 2010, 78 (5) 1931-1942; DOI: 10.1128/IAI.01174-09
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

DNA, Bacterial
Drug Resistance, Multiple, Bacterial
Escherichia coli
Escherichia coli Infections
plasmids
virulence factors
Zoonoses

Related Articles

Cited By...

About

  • About IAI
  • Editor in Chief
  • Editorial Board
  • Policies
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Ethics
  • Contact Us

Follow #IAIjournal

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0019-9567; Online ISSN: 1098-5522