Article Figures & Data
Figures
- FIG. 1.
Resolution of B. hermsii bacteremia in the absence of Cxcl13-mediated migration. Wild-type (n = 5 or 6) or Cxcl13−/− (n = 7 to 9) mice were infected intravenously (A) or intraperitoneally (B) with 5 × 104B. hermsii bacteria (strain DAH-p1), and bacteremia was monitored by dark-field microscopy. Each plot represents data from an individual mouse. For brevity, results for three representative mice from each group are shown.
- FIG. 2.
Comparable B. hermsii-specific IgM responses in wild-type and Cxcl13−/− mice. B. hermsii-specific IgM generated during intravenous (A) or intraperitoneal (B) infection was measured by ELISA. Means ± standard deviations (SD) are shown. FhbA-specific IgM generated during intravenous (C) or intraperitoneal (D) infection was measured by ELISA. Data points in panels C and D represent individual animals, and horizontal bars represent means of results for each group. Statistical significance of the genotype was measured by two-way ANOVA with significance reached at a P of <0.05. The differences between the two genotypes were not statistically significant.
- FIG. 3.
Lack of an expansion of peritoneal B1b cells in B. hermsii-infected Cxcl13−/− mice. Peritoneal cells from naïve and convalescent-phase (at 35 days postinfection) wild-type and Cxcl13−/− mice were harvested and stained with monoclonal antibodies specific for IgM, CD23, CD5, and Mac1. The absolute cell counts of IgM+ B cells (A), B1b cells (IgM+ CD23− Mac1+ CD5−) (B), and B1a cells (IgM+ CD23− Mac1+ CD5+) (C) are shown. Data points represent cell counts from individual animals. Statistical significance was determined by a one-tailed unpaired Student's t test, with significance reached at a P of <0.05. n.s., differences not statistically significant.
- FIG. 4.
Pneumovax 23 (PVAX) elicits a protective antibody response in Cxcl13−/− mice. Wild-type and Cxcl13−/− mice were immunized intraperitoneally with 10 μg of Pneumovax 23. Blood samples were obtained on 0, 7, and 14 days postimmunization and Pneumovax 23 (A)- or PPS3 (B)-specific IgM levels were determined by ELISA. Data points represent individual animals, and horizontal bars represent means of results for each group. The statistical significance of genotypes was measured by two-way ANOVA, with significance reached at a P of <0.05. (C) Four weeks following Pneumovax 23 immunization, mice were challenged with 5,000 CFU of S. pneumoniae WU2 (serotype 3) and survival was monitored for 10 days. The differences between the results for the two genotypes were not statistically significant.
- FIG. 5.
NP-specific IgM responses in Cxcl13−/− mice. Wild-type and Cxcl13−/− mice were immunized intraperitoneally with 50 μg of 50NP-Ficoll. Blood samples were obtained on 0 (preimmune) or 7 (immune) days following the immunization, and 23NP-specific IgM levels were determined by ELISA. Data points represent individual mice, and horizontal bars represent means of results for each group. Statistical significance of the response to immunization was determined by a two-tailed unpaired Student's t test. n.s., not statistically significant.
Tables
- TABLE 1.
Frequencies of NP-specific B cells in various anatomic compartmentsa
Compartment Frequency of IgM+ NP+ cells inb: Significance (P) C57BL/6 mice (n = 5) Cxcl13−/− mice (n = 6) Peritoneal cavity 0.40 ± 0.19 0.22 ± 0.16 n.s.c Blood 0.063 ± 0.016 0.16 ± 0.041 0.0043 MLNs 0.043 ± 0.019 0.088 ± 0.030 0.0303 Spleen 0.11 ± 0.030 0.16 ± 0.028 0.0441 ↵ a Wild-type (n = 5) and Cxcl13−/− (n = 6) mice were immunized i.p. with NP-Ficoll. Five to 6 days postimmunization, cells were harvested from various compartments of mice and stained with NP-phycoerythrin and anti-IgM-FITC.
↵ b Means ± SDs of IgM+ NP+ cell frequencies are given.
↵ c n.s., not significantly different.
Supplemental material
Files in this Data Supplement:
- Supplemental file 1
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Fig. S1. Cxcl13−/− mice are resistant to reinfection with B. hermsii.
JPEG Image file, 417K. - Supplemental file 2
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Legend for Fig. S1.
MS Word document, 22K.
- Supplemental file 1
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Fig. S1. Cxcl13−/− mice are resistant to reinfection with B. hermsii.
