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Bacterial Infections

Relative Contributions of Ebp Pili and the Collagen Adhesin Ace to Host Extracellular Matrix Protein Adherence and Experimental Urinary Tract Infection by Enterococcus faecalis OG1RF

Sreedhar R. Nallapareddy, Kavindra V. Singh, Jouko Sillanpää, Meng Zhao, Barbara E. Murray
A. Camilli, Editor
Sreedhar R. Nallapareddy
1Division of Infectious Diseases, Department of Internal Medicine, University of Texas Medical School, Houston, Texas 77030
2Center for the Study of Emerging and Reemerging Pathogens, University of Texas Medical School, Houston, Texas 77030
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Kavindra V. Singh
1Division of Infectious Diseases, Department of Internal Medicine, University of Texas Medical School, Houston, Texas 77030
2Center for the Study of Emerging and Reemerging Pathogens, University of Texas Medical School, Houston, Texas 77030
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Jouko Sillanpää
1Division of Infectious Diseases, Department of Internal Medicine, University of Texas Medical School, Houston, Texas 77030
2Center for the Study of Emerging and Reemerging Pathogens, University of Texas Medical School, Houston, Texas 77030
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Meng Zhao
1Division of Infectious Diseases, Department of Internal Medicine, University of Texas Medical School, Houston, Texas 77030
2Center for the Study of Emerging and Reemerging Pathogens, University of Texas Medical School, Houston, Texas 77030
3Department of Microbiology and Molecular Genetics, University of Texas Medical School, Houston, Texas 77030
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Barbara E. Murray
1Division of Infectious Diseases, Department of Internal Medicine, University of Texas Medical School, Houston, Texas 77030
2Center for the Study of Emerging and Reemerging Pathogens, University of Texas Medical School, Houston, Texas 77030
3Department of Microbiology and Molecular Genetics, University of Texas Medical School, Houston, Texas 77030
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  • For correspondence: bem.asst@uth.tmc.edu
A. Camilli
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DOI: 10.1128/IAI.00038-11
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ABSTRACT

Previous studies have demonstrated that the ebp operon and the ace gene of Enterococcus faecalis, encoding endocarditis- and biofilm-associated pili and an adhesin to collagen of E . faecalis, respectively, are both important in experimental urinary tract infections (UTI) and endocarditis. We have also shown that growth of E. faecalis in brain heart infusion (BHI) serum enhances Ebp pilus and Ace production and increases adherence to several host extracellular matrix proteins. Here, we report that deletion of ebpABC almost eliminated serum-elicited adherence to fibrinogen (P < 0.0001), resulted in moderate reduction in adherence to collagen (P < 0.05), and had no effect on fibronectin adherence relative to that of wild-type OG1RF. An OG1RFΔaceΔebpABC double mutant showed further reduced collagen adherence versus that of the OG1RFΔace or OG1RFΔebpABC mutants (P < 0.001). These results were corroborated by complementation and/or studies with native pilus-enriched surface extracts and a collagen-secreting 3T6 fibroblast cell line, as well as antibody inhibition. In the UTI model, both the OG1RFΔace and OG1RFΔaceΔebpABC mutants were found to be significantly attenuated compared to the wild type; however, no significant differences were observed between individual ace or ebp mutants and the OG1RFΔaceΔebpABC mutant. In summary, these data implicate the Ebp pili as having some role in collagen adherence, albeit less than that of Ace, and a very major role in fibrinogen adherence, which may explain in part the importance of these pili in experimental endocarditis. The OG1RFΔaceΔebpABC mutant was attenuated in the UTI model, although not significantly more so than the Δace or ΔebpABC mutants, suggesting involvement of other E. faecalis factors in urinary tract colonization or infection.

FOOTNOTES

    • Received 10 January 2011.
    • Returned for modification 18 February 2011.
    • Accepted 7 April 2011.
    • Accepted manuscript posted online 19 April 2011.
  • ↵† Supplemental material for this article may be found at http://dx.doi.org/10.1128/IAI.00038-11.

  • Copyright © 2011, American Society for Microbiology
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Relative Contributions of Ebp Pili and the Collagen Adhesin Ace to Host Extracellular Matrix Protein Adherence and Experimental Urinary Tract Infection by Enterococcus faecalis OG1RF
Sreedhar R. Nallapareddy, Kavindra V. Singh, Jouko Sillanpää, Meng Zhao, Barbara E. Murray
Infection and Immunity Jun 2011, 79 (7) 2901-2910; DOI: 10.1128/IAI.00038-11

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Relative Contributions of Ebp Pili and the Collagen Adhesin Ace to Host Extracellular Matrix Protein Adherence and Experimental Urinary Tract Infection by Enterococcus faecalis OG1RF
Sreedhar R. Nallapareddy, Kavindra V. Singh, Jouko Sillanpää, Meng Zhao, Barbara E. Murray
Infection and Immunity Jun 2011, 79 (7) 2901-2910; DOI: 10.1128/IAI.00038-11
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