Fig 7

The PPE38 mutant caused less-severe pathology. Shown are histopathologies of zebrafish infected by WT M. marinum (A to H) and PPE38 mutant strain 05B1 (I to P). Three fish at day 15 postinfection were sacrificed and subjected to histological analysis with hematoxylin and eosin staining (A, B, C, D, I, J, K, and L) and Ziehl-Neelson staining (E, F, G, H, M, N, O, and P) of serial sections. Arrows indicate bacteria outside granulomas, while arrowheads show bacteria inside granulomas. Magnifications, ×64 for all sections.