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Bacterial Infections

The Intracellular Cyclophilin PpiB Contributes to the Virulence of Staphylococcus aureus Independently of Its Peptidyl-Prolyl cis/trans Isomerase Activity

Rebecca A. Keogh, Rachel L. Zapf, Richard E. Wiemels, Marcus A. Wittekind, Ronan K. Carroll
Nancy E. Freitag, Editor
Rebecca A. Keogh
aDepartment of Biological Sciences, Ohio University, Athens, Ohio, USA
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Rachel L. Zapf
aDepartment of Biological Sciences, Ohio University, Athens, Ohio, USA
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Richard E. Wiemels
aDepartment of Biological Sciences, Ohio University, Athens, Ohio, USA
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Marcus A. Wittekind
aDepartment of Biological Sciences, Ohio University, Athens, Ohio, USA
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Ronan K. Carroll
aDepartment of Biological Sciences, Ohio University, Athens, Ohio, USA
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  • ORCID record for Ronan K. Carroll
Nancy E. Freitag
University of Illinois at Chicago
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DOI: 10.1128/IAI.00379-18
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ABSTRACT

The Staphylococcus aureus cyclophilin PpiB is an intracellular peptidyl prolyl cis/trans isomerase (PPIase) that has previously been shown to contribute to secreted nuclease and hemolytic activity. In this study, we investigated the contribution of PpiB to S. aureus virulence. Using a murine abscess model of infection, we demonstrated that a ppiB mutant is attenuated for virulence. We went on to investigate the mechanism through which PpiB protein contributes to virulence, in particular the contribution of PpiB PPIase activity. We determined the amino acid residues that are important for PpiB PPIase activity and showed that a single amino acid substitution (F64A) completely abrogates PPIase activity. Using purified PpiB F64A protein in vitro, we showed that PPIase activity only partially contributes to Nuc refolding and that PpiB also possesses PPIase-independent activity. Using allelic exchange, we introduced the F64A substitution onto the S. aureus chromosome, generating a strain that produces enzymatically inactive PpiB. Analysis of the PpiB F64A strain revealed that PPIase activity is not required for hemolysis of human blood or virulence in a mouse. Together, these results demonstrate that PpiB contributes to S. aureus virulence via a mechanism unrelated to prolyl isomerase activity.

FOOTNOTES

    • Received 10 July 2018.
    • Returned for modification 30 July 2018.
    • Accepted 6 August 2018.
    • Accepted manuscript posted online 13 August 2018.
  • Supplemental material for this article may be found at https://doi.org/10.1128/IAI.00379-18.

  • Copyright © 2018 American Society for Microbiology.

All Rights Reserved.

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The Intracellular Cyclophilin PpiB Contributes to the Virulence of Staphylococcus aureus Independently of Its Peptidyl-Prolyl cis/trans Isomerase Activity
Rebecca A. Keogh, Rachel L. Zapf, Richard E. Wiemels, Marcus A. Wittekind, Ronan K. Carroll
Infection and Immunity Oct 2018, 86 (11) e00379-18; DOI: 10.1128/IAI.00379-18

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The Intracellular Cyclophilin PpiB Contributes to the Virulence of Staphylococcus aureus Independently of Its Peptidyl-Prolyl cis/trans Isomerase Activity
Rebecca A. Keogh, Rachel L. Zapf, Richard E. Wiemels, Marcus A. Wittekind, Ronan K. Carroll
Infection and Immunity Oct 2018, 86 (11) e00379-18; DOI: 10.1128/IAI.00379-18
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KEYWORDS

PPIase
S. aureus
cyclophilin
nuclease
PpiB
Staphylococcus aureus
chaperone
micrococcal nuclease

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