Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About IAI
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Infection and Immunity
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About IAI
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Microbial Immunity and Vaccines

B Cell Presentation of Chlamydia Antigen Selects Out Protective CD4γ13 T Cells: Implications for Genital Tract Tissue-Resident Memory Lymphocyte Clusters

Raymond M. Johnson, Hong Yu, Norma Olivares Strank, Karuna Karunakaran, Ying Zhu, Robert C. Brunham
Andreas J. Bäumler, Editor
Raymond M. Johnson
aSection of Infectious Diseases, Department of Medicine, Yale University School of Medicine, New Haven, Connecticut, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hong Yu
cVaccine Research Laboratory, University of British Columbia Centre for Disease Control, Vancouver, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Norma Olivares Strank
aSection of Infectious Diseases, Department of Medicine, Yale University School of Medicine, New Haven, Connecticut, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Karuna Karunakaran
cVaccine Research Laboratory, University of British Columbia Centre for Disease Control, Vancouver, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ying Zhu
bDepartment of Biostatistics, Yale University School of Medicine, New Haven, Connecticut, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Robert C. Brunham
cVaccine Research Laboratory, University of British Columbia Centre for Disease Control, Vancouver, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Andreas J. Bäumler
University of California, Davis
Roles: Editor
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/IAI.00614-17
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

ABSTRACT

Surveillance and defense of the enormous mucosal interface with the nonsterile world are critical to protecting the host from a wide range of pathogens. Chlamydia trachomatis is an intracellular bacterial pathogen that replicates almost exclusively in the epithelium of the reproductive tract. The fallopian tubes and vagina are poorly suited to surveillance and defense, with limited immune infrastructure positioned near the epithelium. However, a dynamic process during clearing primary infections leaves behind new lymphoid clusters immediately beneath the epithelium. These memory lymphocyte clusters (MLCs) harboring tissue-resident memory (Trm) T cells are presumed to play an important role in protection from subsequent infections. Histologically, human Chlamydia MLCs have prominent B cell populations. We investigated the status of genital tract B cells during C. muridarum infections and the nature of T cells recovered from immune mice using immune B cells as antigen-presenting cells (APCs). These studies revealed a genital tract plasma B cell population and a novel genital tract CD4 T cell subset producing both gamma interferon (IFN-γ) and interleukin-13 (IL-13). A panel of CD4 T cell clones and microarray analysis showed that the molecular fingerprint of CD4γ13 T cells includes a Trm-like transcriptome. Adoptive transfer of a Chlamydia-specific CD4γ13 T cell clone completely prevented oviduct immunopathology without accelerating bacterial clearance. Existence of a CD4γ13 T cell subset provides a plausible explanation for the observation that human peripheral blood mononuclear cell (PBMC) Chlamydia-specific IFN-γ and IL-13 responses predict resistance to reinfection.

INTRODUCTION

Chlamydia trachomatis infections of the reproductive tract have evaded public health interventions for the past several decades. In the United States and Canada, the incidence of C. trachomatis infections continues to climb despite effective antibiotics and public health measures that increased screening, partner notification, and treatment. In fact, the attempt to control C. trachomatis infection likely aborts the development of herd immunity and results in the need to treat even great numbers of individuals (1, 2); arrested immunity due to doxycycline treatment is demonstrable in the Chlamydia muridarum mouse model (3). It is widely accepted by researchers and public health officials that the only intervention likely to reduce the incidence of disease and the human toll and expense inflicted by C. trachomatis-induced infertility and ectopic pregnancy is a Chlamydia vaccine. While much progress has been made, the immunologic goals of a Chlamydia vaccine remain elusive, and no human vaccine against the urogenital serovars has been attempted. The finding that untreated humans can self-clear genital tract infections (4–6) and that those who do are less likely to be reinfected (7) provides proof in principle for a Chlamydia genital tract vaccine.

The immunologic goal of vaccination for protective immunity against urogenital serovars is likely a multifunctional Th1 response (8). The role of antibodies in a future C. trachomatis vaccine is unclear, with animal model data supporting (9–12) and refuting (13–15) a role for Chlamydia-specific antibodies in protective immunity absent a preexisting T cell response. In human studies, we and others have shown that IgG and IgA antibody responses measured in serum do not correlate with protective immunity (16–18), and a prospective human clinical investigation showed a linear positive correlation between antichlamydial antibody titers and future infertility (19). In mice, CD8 T cell responses are associated with immunopathology rather than protection (20–23), although there are caveats to this statement, including evidence for CD8 protection with a trachoma vaccine in macaques (24) and the identification of CD8 epitopes that correlate with self-resolution in humans (25). While many questions remain about the pathophysiology of protection versus immunopathology, it is generally accepted that the reliably protective arm of the adaptive immune response is the CD4 T cell response (26, 27).

A critical component for rational vaccine development is a surrogate biomarker for protective immunity. For early successful vaccines like the hepatitis B virus vaccine, the surrogate biomarker was a relatively easily determined antibody titer to the hepatitis B surface antigen. A practicable surrogate biomarker for protective immunity is defined as a testable parameter that can be reasonably and reliably measured after administration of a vaccine that correlates with resistance to infection. Currently there are only two such surrogate biomarkers for C. trachomatis immunity defined by Cohen et al. in a longitudinal study of Kenyan sex workers (18): a peripheral blood mononuclear cell (PBMC) gamma interferon (IFN-γ) response to Chlamydia heat shock protein 60 (HSP60), which is not useful in the context of vaccines as HSP60 is an unlikely candidate component of a subunit vaccine, and a PBMC interleukin-13 (IL-13) response to the elementary body (EB [i.e., the infectious form of C. trachomatis]). The latter has been an enigma as IL-13 is a Th2 cytokine, and Th2 responses are associated with negative outcomes in animal models of Chlamydia infection (28, 29).

In the context of an emerging new understanding of mucosal host defense based on local adaptive immunity mediated by tissue-resident memory (Trm) T cells, we recently revisited the Chlamydia genital tract pathogenesis paradigm with a Trm rather than cytokine polarization Th1/2/17 framework and reported our unpublished observation that the Chlamydia-specific CD4 T cell response includes a population of CD4 T cells that produce IFN-γ and IL-13 (30). We postulated that CD4γ13 T cells reflected a Trm response and, based on the data of others (31–33), that the Chlamydia memory lymphocyte clusters include immune plasma B cells as antigen-presenting cells (APCs). We present the discovery and characterization of CD4γ13 T cells here.

RESULTS

Plasma cells in the genital tract.We recently revisited the Chlamydia pathogenesis literature through the lens of tissue-resident immunity rather than cytokine polarization (Th1/2/17), highlighting human studies by others showing B lymphocytes and plasma B cells are prominent in Chlamydia infection-associated memory lymphocyte clusters (c-MLCs) (30). B lymphocyte data in the C. muridarum mouse model are inconclusive due to utilization of staining with B220, a marker downregulated when B lymphocytes transition to immune plasma B cells. To address the discrepancy between human and mouse data, we determined B cell dynamics in the genital tract over the course of a C. muridarum infection, gating on CD79a and measuring the relative levels of B lymphocytes (high B220 expression) and plasma B cells (low B220 expression) (Fig. 1A; [see the gating strategy in Fig. S1 in the supplemental material]). Gating on CD79a allows detection of plasma B cells that do not express B220 (34). In naive mice, very few plasma cells reside in the genital tract. During the course of a C. muridarum genital tract infection, the percentage of plasma cells increases from a baseline of 3% to 13%, with a further expansion to 22% during rechallenge infections. The results in Fig. 1A show that plasma B cells are nearly absent in a naive genital tract and expand as demonstrable immunity develops over the course of a primary infection.

FIG 1
  • Open in new tab
  • Download powerpoint
FIG 1

B cell dynamics in the genital tract during C. muridarum infection and differential expansion of memory T cell subsets. (A) Single-cell suspensions of genital tracts from the following conditions were gated on CD79a (B cells) and analyzed for the level of B220: high expression of B220 indicates B lymphocytes and low expression indicates plasma B cells. Uninfected mice (Naive), day 7 primary infection (D7_pri_inf), day 35 primary infection (D35_pri_Cm inf), and day 5 secondary infection (D5_sec_inf) were investigated. Mice were pretreated with medroxyprogesterone and infected 1 week later with 1,500 IFU of C. muridarum; representative data are shown from a minimum of 2 experiments for each experimental group. (B) Analysis of B cell APC-derived and immune splenocyte-derived Chlamydia-specific polyclonal T cell lines for production of IL-13. T cell lines were activated for 5 h with PMA-ionomycin-brefeldin A-monensin and stained for CD8 versus IL-13. CD4 T cells are identified as CD8neg in this assay (see Materials and Methods).

Immune B cells as antigen-presenting cells.B cells from immunized mice bearing endogenous immunoglobulins (single specificity) and a sampling of serum IgG (multiple specificities) bound to their cell surfaces via Fc receptors (FcRs) can activate T cells at cognate antigen concentrations 1,000-fold lower than do naive B cells (35): i.e., are 1,000× more potent as APCs. For the remainder of the article, we refer to B cells purified from mice that previously self-cleared C. muridarum genital tract infections (immune mice) as “immune B cells.” We investigated the nature of Chlamydia-specific T cells recovered from immune mice using immune B cells as APCs; the use of splenocytes rather than genital tract lymphocytes was based on limited cell numbers in genital tract tissue and the need to develop untested methodologies. We purified splenic B cells from an immune mouse, pulsed them with UV-inactivated Chlamydia muridarum (uvMoPn), and then washed them extensively (400,000-fold) to remove all antigen not bound to or internalized by the immune B cells, thereby limiting antigen presentation to B cells. We cocultured antigen-pulsed/washed B cells (immune B cell APC) with splenocytes from the same immune mouse in two primary wells to expand T cells. In parallel, for comparison, from the same mouse, we expanded T cells in 2 primary wells using uvMoPn and unfractionated immune splenocytes (immune splenocyte APC) as we have previously published (36). At passage 3, we did flow cytometry to determine relative CD4/CD8 numbers: >95% of the resulting T cell populations from the expansions based on the immune B cell APC line and the expansions based on the immune splenocyte APC line were CD4 T cells (see Fig. S2 in the supplemental material). Although none of our published or unpublished Chlamydia-specific CD4 T cell murine studies had evidence for a CD4 IL-13 T cell response, we were interested in IL-13 because of its association with immune protection and pathology. Upon activation, the two immune B cell APC-derived polyclonal T cell lines produced IL-13, while the two immune splenocyte APC-derived T cell populations did not (data not shown). We propagated the two immune B cell APC-derived T cell lines and generated immune splenocyte APC-derived polyclonal T cell lines from four additional mice that previously cleared C. muridarum genital tract infections. We activated two immune B cell APC-derived and four immune splenocyte APC-derived polyclonal T cell lines with phorbol myristate acetate (PMA)-ionomycin and stained CD8 versus IL-13 (negative CD8 staining was intentionally used to identify CD4 T cells in this assay [see Materials and Methods]) (Fig. 1B). The immune B cell APC-derived polyclonal lines included a subset of CD4 T cells producing IL-13; none of the four immune splenocyte APC-derived polyclonal T cell lines had a CD4 IL-13 T cell subset. We activated one immune B cell APC-derived T cell line and one immune splenocyte APC-derived T cell line from the same mouse and did intracellular staining for IFN-γ and IL-13 (Fig. 2A). The immune splenocyte APC-derived CD4 T cell line did not stain for IL-13; all the T cells in the immune B cell APC-derived T cell line that produced IL-13 also produced IFN-γ. We activated an immune B cell APC-derived T cell line (B2) and five immune splenocyte APC-derived T cell lines (spl1 to -5) with purified uvMoPn-pulsed immune B cells and measured IFN-γ, IL-13, and IL-4 in culture supernatant (Fig. 2B). All of the polyclonal T cell lines were Chlamydia specific: when activated by antigen-pulsed B cells, they all produced IFN-γ. Only the immune B cell APC-derived T cell line B2 produced IL-13. None produced IL-4. We empirically noted that IL-13 production in immune B cell APC-derived T cell lines faded with increasing passage number (not shown) and determined that durable IL-13 production by immune B cell APC-derived polyclonal T cell lines required addition of transforming growth factor β1 (TGF-β1) to the medium (see Fig. S3 in the supplemental material) and that the TGF-β1 effect was specific to T cell lines derived from the immune B cell APC combination: i.e., addition of TGF-β1 to immune splenocyte APC-derived T cell lines did not result in IL-13 production (data not shown).

FIG 2
  • Open in new tab
  • Download powerpoint
FIG 2

Cytokine profile of B cell-derived polyclonal B2 versus immune splenocyte-derived polyclonal CD4 T cell lines, shown by the presence of CD4γ13 T cells in the genital tract and spleen. (A) Polyclonal B cell-derived line 2 (B2) and splenocyte-derived line 2 (spl2) were activated for 5 h with PMA-ionomycin-brefeldin A-monensin and then stained for CD8 (negative stain for CD4 T cells) versus IL-13 and IFN-γ versus IL-13. (B) Specificity and cytokine profiles for B2 and five splenocyte-derived T cell lines (spl1 to -5). 2.5 × 104 T cells were coincubated with 5 × 104 purified immune B cells unpulsed (APCs) or pulsed with uvMoPn (antigen [Ag]) in triplicate. Cell culture supernatants harvested at 48 h, and levels of IFN-γ, IL-13, and IL-4 were determined by ELISA. Shown are comparisons between control (Con) and antigen-pulsed (Ag) B cell activation for each cytokine and T cell line. *, P < 0.05; ****, P < 0.0005. (C) Frequency of IL-13+ CD4 T cells in the spleen and genital tract. C57BL/6 female mice in experimental groups of 4 mice each were PmpG immunized (PmpG Imm), unimmunized (Prim. Infect), or previously infected with C. muridarum (1,500 IFU vaginally [Sec. Infect]). Four weeks after the last immunization or C. muridarum primary infection, mice were challenged with intravaginally with C. muridarum. Six days later, single-cell suspensions prepared from individual mice (except naive uninfected control) and CD4 T cells were scored for IFN-γ, TNF-α, IL-13, and IFN-γ/IL-13. (D) For genital tract CD4γ13 T cells, the left panel shows the percentage that also produce IFN-γ and the right panel shows the intensity of IFN-γ production. Data for individual mice are shown in Table S1.

We next investigated whether there was a CD4γ13 T cell response to Chlamydia infection systemically (spleen) and locally (genital tract). CD4 T cell responses in the genital tract and spleen, quantified for IFN-γ, tumor necrosis factor alpha (TNF-α), and IL-13, were determined for naive, PmpG-immunized, and immune mice (i.e., mice that had cleared prior infection) on day 6 post-C. muridarum infection (Fig. 2C; see the gating strategy in Fig. S4 and data for individual mice in Table S1 in the supplemental material). CD4γ13 T cells were present but rare in the spleen. In the genital tract during primary infection, 1 to 2% of CD4 T cells were IL-13+, which increased to 5 to 10% with PmpG-dimethyldioctadecylammonium bromide (DDA)-trehalose dibehenate (TDB) immunization and were maximal during secondary infection at 4 to 15%. In naive mice, roughly half of the IL-13+ CD4 T cells were IFN-γ positive: 70 to 80% in PmpG-immunized mice during a primary response and >90% dual cytokine positive in mice during a secondary response (Fig. 2D). CD4γ13 T cells are a significant component of the local mucosal, but not the systemic, CD4 T cell response to primary genital tract infections, and their numbers were enhanced in the setting of preexisting immunity due to prior infection or protective PmpG vaccination.

CD4γ13 T cell clones.Having established that CD4γ13 T cells were a physiologic component of the host response to Chlamydia genital tract infections, we generated CD4 T cell clones using immune B cell antigen presentation to assemble a panel of immune B cell APC-derived multifunctional Th1 and CD4γ13 T cell clones for comparison to each other and to our existing CD4 T cell clones. Using conditions mimicking the polyclonal derivation, modified to incorporate TGF-β1 and using both uvMoPn and soluble Chlamydia antigen, we were able to derive a panel of T cell clones from immune mice. A working panel of six CD4 T cell clones was carried forward including two immune B cell APC-derived CD4γ13 clones (sBT13-7 and sBT16-8), two immune B cell APC-derived multifunctional Th1 clones that did not produce IL-13 (BT12-7 and sBT13-11), an immune B cell APC-derived CD4 clone that lost IL-13 production over time (BT12-17), and a multifunctional Th1 clone derived with unfractionated splenocyte APCs that we previously described (4uvmo-3) (37). The CD4 T cell clones were activated with immobilized anti-CD3, and then levels of IL-2, IFN-γ, IL-13, IL-10, TNF-α, IL-17, IL-22, IL-4, and IL-5 in culture supernatant were determined by enzyme-linked immunosorbent assay (ELISA) (Fig. 3A). The two CD4γ13 clones shared IL-2, IFN-γ, IL-13, IL-10, and TNF-α and split on IL-17 plus IL-22 versus IL-4 plus IL-5. Only one of the CD4γ13 T cell clones produced IL-4: sBT16-8. Even at high cell density, no IL-4 was detectable upon activation of the CD4γ13 clone sBT13-7. No overarching statement can be made about CD4 T cell cytokine polarization in the panel, except that each clone had a unique profile. Th2 cells have a T cell receptor (TCR)-independent pathway for IL-13 production based on prostaglandin D binding the CrTh2 receptor (38). As CD4γ13 T cells have not been previously described, we tested whether IL-13 production was calcineurin dependent and whether the known calcineurin-independent prostaglandin D-CrTh2 pathway could account for IL-13 production (Fig. 3B). Compared to IFN-γ, IL-13 production was significantly less inhibited by cyclosporine (CsA). Based on small molecule inhibitors, the residual IL-13 production in the presence of CsA was not due to the CrTh2 pathway.

FIG 3
  • Open in new tab
  • Download powerpoint
FIG 3

Cytokine profiles of Chlamydia-specific CD4 clones, IL-13 production, and replication control. 2.5 × 104 T cells were activated with immobilized anti-CD3 antibody in 96-well plates; 5 × 104 T cells/well were used for IL-4 to increase sensitivity. Twenty-hour culture supernatants were analyzed for IL-2 (yellow), IFN-γ (black), IL-13 (green), IL-10 (gray hatched), TNF-α (light blue hatched), IL-17 (red), IL-22 (dark blue), IL-5 (orange), and IL-4 (pink). All visible bars are significant (P < 0.05) compared to parallel wells lacking anti-CD3 antibodies. The data presented are aggregated from two independent experiments. (B) The CD4γ13 IL-13 pathway is partially calcineurin independent. 2.5 × 104 T cells were activated by immobilized anti-CD3 in the absence and presence of 500 nM (2 ng/ml) CsA without or with small molecule inhibitors of CrTh2 (CrTh2-1 and CrTh2-2) at 5 μM (∼50× the 50% inhibitory concentration [IC50]). The top panel shows IL-13 production, the middle panel IFN-γ production, and the bottom panel relative IL-13 versus IFN-γ in the presence of 500 nM CsA. Shown are aggregated data from two independent experiments. *, P < 0.05; **, P < 0.005; ***, P < 0.0005; ****, P < 0.00005. (C) Termination of C. muridarum replication. C57epi.1 epithelial cells, untreated (top) or pretreated with 10 ng/ml IFN-γ for 6 h (middle), were washed and then infected with 2 IFU C. muridarum per cell. Four hours later, 1.5 × 105 T cells (1:1 ratio) were added to each well and to uninfected wells as controls. Twenty-eight hours later, 100 μl supernatant was removed for IFN-γ analysis and wells were harvested into SPG buffer. IFN-γ levels were determined by ELISA and IFU number by culture on McCoy cells. All IFN-γ values (picograms per milliliter) in parentheses are significant (P < 0.05); IFN-γ levels for all CD4 clones cultured on uninfected epithelial cells were <100 pg/ml. Shown are aggregated data from two independent experiments. The bottom panel shows the cytolytic capability of the CD4 clones versus a Chlamydia-specific CD8 T cell clone in redirected lysis. Ten thousand T cells were incubated with 10,000 Fc receptor (FcR)-bearing P815 cells in the absence (spontaneous release) and presence of 0.5 μg/ml anti-CD3 antibody (activation/lysis) in quadruplicate in a 4-h assay based on LDH release. The percentage of specific lysis is shown for each of the T cell clones (single experiment done as quadruplicates).

We investigated the CD4 T cell clones' ability to recognize and terminate C. muridarum replication in epithelial cells (Fig. 3C, top and middle panels) and their relative cytolytic ability compared to a Chlamydia-specific CD8 cytotoxic T lymphocyte (CTL) clone (8uvmo-2) using redirected lysis (Fig. 3C, bottom panel). All the CD4 T cell clones recognized infected epithelial cells and to various degrees terminated C. muridarum replication in them; only the CD4γ13 clone sBT16-8 fell below 50% inhibition. Two CD4 clones terminated C. muridarum without IFN-γ pretreatment; 4uvmo-3 was nearly IFN-γ independent (did not require epithelial cells be pretreated with IFN-γ), consistent with our prior publication (37). All CD4 clones produced Chlamydia-specific IFN-γ under the conditions of the replication termination assay; IL-13 was not detected under these conditions. No conclusions can be drawn about IFN-γ production versus termination efficiency as termination likely reduces T cell activation, as suggested by lower levels of IFN-γ for all CD4 T cell clones when epithelial cells were pretreated with IFN-γ (improved termination efficiency); the highest level of IFN-γ was for sBT13-11 with untreated infected epithelial cells (8,196 pg/ml), an experimental condition in which sBT13-11 did not significantly terminate replication. The CD4 clones were less cytolytic than a conventional CD8 CTL clone, but all had some ability to kill in a short-term assay.

Gene expression microarray analysis of CD4γ13 T cells.Having a panel of CD4γ13 and multifunctional Th1 T cell clones offered the possibility of defining CD4γ13 T cells at the molecular level using gene expression microarray analysis. We chose to do the initial investigation using T cells in their “rested” state as that condition was more likely to reflect T cell differentiation biology (i.e., biomarkers that may be useful in peripheral blood and uninfected tissue). T cells at the end of the usual 7-day culture cycle were purified by Ficoll-Hypaque and plated without antigenic stimulation for an additional 48 h in medium containing recombinant IL-7. Two days later, the wells were harvested and total RNA isolated; the experiment was repeated 4 times to minimize false discovery. The comparators were sBT13-7 and sBT16-8 (CD4γ13), BT12-7 and sBT13-11 (multifunctional Th1 derived with B cell APCs that do not produce IL-13), BT12-17 (multifunctional Th1 that initially made and then lost IL-13 production), and 4uvmo-3 (multifunctional Th1 derived with unfractionated splenocyte APC). The value of BT12-17 was unclear: it represents either plasticity in the CD4γ13 phenotype or a breakthrough dominant second clone from incomplete limiting dilution. At worst, BT12-17 was a third multifunctional CD4 T cell that did not produce IL-13. sBT13-7 and sBT16-8 were derived with soluble Chlamydia antigen and the other clones with uvMoPn. The microarray comparisons were as follows: (i) sBT13-7+sBT16-8 versus BT12-7+sBT13-11 for the comparison CD4γ13 versus multifunctional Th1 (all B cell derived), (ii) sBT13-7+sBT16-8 versus BT12-17 for possible unique insight into IL-13 biology (loss of function), and (iii) sBT13-7+sBT16-8 versus 4uvmo-3 for the comparison CD4γ13 versus conventional multifunctional Th1 (splenocyte APC derived).

The criteria applied to identify genes of interest were (i) a log2 fluorescence signal of >5.0 (mRNA signal above background), (ii) a statistically significant (P <0.01) 3-fold difference (up or down) between the two CD4γ13 clones in aggregate versus non-CD4γ13 clones in all three comparisons, and (iii) the log2 fluorescence signal for both CD4γ13 clones had to be greater than the individual log2 signals for all the other T cell clones in the array (which eliminates genes skewed by very high or low expression by one of the CD4γ13 T cell clones). Analysis of the microarray data showed that the CD4γ13 T cell clones had more genes in common with each other than the other clones (Fig. 4). Genes with significantly enhanced mRNA signal in CD4γ13 T cells are shown in Table 1, and those with significantly reduced mRNA signal are shown in Table 2. All gene mRNA differences in Table 1 and Table 2 are statistically significant, with the highest false-discovery rates (FDRs) being 7 × 10−4 (Trib2) for the upregulated genes and 1 × 10−4 (S1pr1) for the downregulated genes. The cytokine data for CD4γ13 T cell clones (Fig. 3A) showed unusual combinations of Th1/2/17/22 cytokines, so we parsed out CD4γ13 T cell clone differentiation markers/transcription factors from the microarray (Table 3) and did Western blotting for Tbet (Th1), Gata3 (Th2), Eomes, and Fhl2 on the two CD4γ13 T cell clones (sBT13-7 and sBT16-8) and three IL-13-negative controls (4uvmo-3, BT12-7, and BT12-17) on day 5 of their usual 7-day culture cycle (peak T cell numbers in well). Differentiation markers/transcription factors in the microarray with mRNA signals that were negligible, RORγT (Th17), or low and mismatched between the two CD4γ13 clones, Ahr (Th22), were not included in Western blot analysis; blotting with commercial antibodies for Epas1 generated low-quality blots, and therefore the results are not included in Fig. 5. Transcriptionally the CD4γ13 T cell clones look like Trm (Klrg1neg, Klf2neg, Hnflaneg, S1pr1neg, Ccr7neg; Hobitneg, Blimp-1pos, Rgs1pos, Rgs2pos, Cd69pos, and Cd44pos). Based on limited data, Chlamydia-specific multifunctional Th1 derived from mice that self-cleared a genital tract infection universally express Gata3 and Eomes. Interestingly Tbet expression, as detectable at the level of Western blotting, does not appear to be required for IFN-γ production, although its relative absence may be required for IL-4 production (e.g., sBT16-8), and Gata3 in Chlamydia-specific multifunctional Th1 does not denote a conventionally defined Th2 phenotype or IL-4 production. Fhl2 appears to be the transcription factor that qualitatively and perhaps quantitatively, denotes a CD4γ13 T cell's ability to uniquely produce IL-13.

FIG 4
  • Open in new tab
  • Download powerpoint
FIG 4

Clustering of the top 1,000 genes by the ANOVA P value (i.e., genes that were differentially expressed in at least one clone) shows the two CD4γ13 T cell clones sBT13-7 and sBT16-8 (top two vertical bars on left) are the most alike among the six CD4 T cell clones in the panel (from left to right, blue-red-blue-red-blue pattern).

View this table:
  • View inline
  • View popup
TABLE 1

Genes with significantly enhanced mRNA signal in CD4γ13 T cells

View this table:
  • View inline
  • View popup
TABLE 2

Genes with significantly reduced mRNA signal in CD4γ13 T cells

View this table:
  • View inline
  • View popup
TABLE 3

Expression of CD4γ13 differentiation markers and transcription factors

FIG 5
  • Open in new tab
  • Download powerpoint
FIG 5

Protein levels of selected differentiation/transcription factors. On day 5 of the usual culture cycle, the indicated CD4 T cell clones were purified by Ficoll-Hypaque, and whole-cell lysates were prepared and utilized for immunoblotting for Tbet, Gata3, Eomes, and Fhl2. Blots were stripped and reprobed with anti-β-actin as the loading control. Molecular mass markers (M) in kilodaltons are indicated in the right margin.

Adoptive transfer of multifunction Th1 versus CD4γ13.To determine whether CD4γ13 T cells were capable of protecting or causing genital tract pathology during C. muridarum infections, we adoptively transferred them into naive C57BL/6 mice and challenged the next day with genital tract infections. For comparison, we adoptively transferred 4uvmo-3, the conventional multifunctional Th1 comparator that we'd previously predicted to be protective based on Plac8 positivity, early and relatively IFN-γ-independent recognition of infected epithelial cells, and efficient termination of Chlamydia replication in them (39). Our initial experiment and a staggered/stacked-replicate second experiment were focused on 4uvmo-3 (splenic APC-derived multifunctional Th1) versus sBT16-8 (CD4γ13 with the highest IL-13 production), piloting smaller numbers of mice with sBT13-7 (other CD4γ13 clone) and BT12-17 (B cell-derived multifunctional Th1 without IL-13). When the first experiment reached 8 weeks and was scored for pathology, it was clear that sBT13-7 was likely the most protective T cell clone (zero pathology in three mice; control incidence of ≥60%). A third cohort of control and sBT13-7 mice was initiated to complete the data set. Mice were monitored for bacterial shedding through day 30 (Fig. 6A and B) and killed on day 56 to score immunopathology (Fig. 6C [dissected genital tracts in panel D]). The multifunctional Th1 clone 4uvmo-3 was partially protective, reducing the frequency of hydrosalpinx from ∼60% of oviducts to ∼20%. sBT13-7, a CD4γ13 T cell clone, dramatically protected mice from C. muridarum immunopathology, preventing damage to uteri in 8 of 9 mice and preventing hydrosalpinx in 9 of 9 mice. sBT16-8, the other CD4γ13 T cell clone, was neither protective or pathological. Interestingly the protection afforded to the murine genital tract by sBT13-7 and 4uvmo-3 was largely limited to oviducts and did not correlate with rapidity of bacterial clearance.

FIG 6
  • Open in new tab
  • Download powerpoint
FIG 6

Adoptive transfer as shown by bacterial shedding and pathology scoring. (A) IFU shedding in the lower genital tract. (B) Percentage of mice shedding at each time point. (C) On day 56, mice were killed and uterine and oviduct pathology was scored in situ (see Materials and Methods). (D) Digital images of the oviducts; black arrows indicate scored pathology. *, P < 0.05; **, P < 0.005; ***, P < 0.0005. Shown are aggregated data from 3 experiments: control (14 mice), 4uvmo-3 (9 mice), sBT13-7 (9 mice), and sBT16-8 (8 mice). Txf, adoptive transfer.

DISCUSSION

We investigated B cells, CD4 T cells, and IL-13 during Chlamydia infections of the genital tract in the context of tissue-resident mucosal immunity. The impetus for this research was the paradoxical data regarding Th2 cells and IL-13 in Chlamydia host defense. On one hand, Th2 responses to Chlamydia infections have been associated with ineffectual or worsened pathological outcomes in mouse models (28, 29), including a study showing that IL-13 knockout mice cleared infections more rapidly with less pathology than wild-type mice (40). On the other hand, our human clinical investigation showed that a PBMC IL-13 response to EBs prospectively identified individuals resistant to reinfection with C. trachomatis (18), supporting a protective role for presumably T cells polarized to produce IL-13. Data presented here show these disparate results are biologically compatible and call into question the utility of the Th1/2/17 cytokine polarization framework for understanding genital tract immunity during Chlamydia infections.

Mice deficient in B cells clear primary C. muridarum infections in the usual time frame, with the caveat that they develop a transient peritonitis and early dissemination (41); mice deficient in B cells remain susceptible to reinfection with a clearance time only slightly faster than that of naive mice during primary infections (14). Published immunohistochemical analysis of lymphoid aggregates in human and mouse genital tract and conjunctival tissues in the setting of current or prior Chlamydia infections documents abundant B cells in Chlamydia-specific memory lymphocyte clusters (c-MLCs) (reviewed in reference 30). The combination of B cell knockout mouse susceptibility to reinfection and the abundance of B cells in c-MLCs suggest that B cells play an important role in protective secondary immune responses (42). In the original mouse work by Morrison and Morrison based on B220 immunohistochemical staining, B cells were present during the first 2 weeks of infection but disappeared in subsequent weeks. B220 is downregulated as activated B lymphocytes transition to immune plasma B cells (34). We analyzed the status of B lymphocytes and plasma B cells over the time course of infection by doing flow cytometry on single-cell suspensions generated from uteri and oviducts. We used the pan-B cell marker CD79a to identify B cells in toto and B220 to characterize them as B lymphocytes (high B220 expression) or plasma B cells (low B220 expression). We found that plasma B cells are nearly absent in naive genital tract tissue, become detectable during primary infection, and markedly expand during infections in mice with preexisting immunity generated either by PmpG-DDA-TDB vaccination or prior infection. The kinetics of plasma B cell expansion mirror the time course of demonstrable T cell immunity to C. muridarum (3) and are compatible with plasma B cells playing a role in Chlamydia-specific MLC (c-MLC) and protective immunity. These results suggest that our novel T cell recovery/expansion protocol based on immune B cell APC is physiologically relevant.

Immune B cell presentation of Chlamydia antigens to T cells from mice that previously cleared genital tract infections preferentially expanded CD4 T cells over CD8 T cells, including a subset polarized to produce IL-13 and IFN-γ. Analysis of CD4 T cells in the mouse genital tract and spleen in the naive state, vaccinated state, and postclearance of a primary C. muridarum infection showed that CD4γ13 T cells were localized to the genital tract in physiologically relevant levels: up to 15% of Chlamydia-specific T cells, a 500-fold enrichment of CD4γ13 T cells in the genital tract tissue versus the spleen. Importantly, immunization with the protective PmpG in DDA-TDB vaccine at the base of the tail enhanced the presence of CD4γ13 T cells in the genital tract.

Using the immune B cell APC protocol, we generated a panel of CD4 T cell clones from the spleens of immune mice (with prior genital tract infection) that included two CD4γ13 T cells, thereby providing an opportunity to define the CD4γ13 T cell subset at the molecular level using gene expression microarray analysis. The microarray data suggest a CD4γ13 memory T cell subset with Trm-like differentiation (Klrg1neg, Klf2neg, Hnflaneg, S1pr1neg, Ccr7neg; Hobitneg, Blimp-1pos, Rgs1pos, Rgs2pos, Cd69pos, and Cd44pos) (43). We postulate that the CD4γ13 T cell clones are progeny of the contraction of the CD4 T cell response during the primary genital tract infection—i.e., Trm T cells trafficked to the spleen to serve as a Trm repository reflecting events that occurred in the genital tract. Within the microarray, CD4γ13 T cell clones were more like each other than the other T cells in the panel, but were not homogenous. At the cytokine level, sBT13-7 and sBT16-8 shared at least 5 cytokines (IL-2, IFN-γ, IL-13, IL-10, and TNF-α), with sBT13-7 adding IL-17 and IL-22 and sBT16-8 adding IL-4 and IL-5. Unlike IL-2, IL-4, or IFN-γ, T cell production of IL-13 is partially resistant to cyclosporine (CsA). In human peripheral blood CD4 T cells, CsA-resistant IL-13 production occurs only at low concentrations, <30 nM, through a MEK-dependent pathway (44). The CD4γ13 T cells we report here continued to preferentially produce IL-13 versus IFN-γ in experiments using 500 nM CsA, >10 times the concentration that completely blocked IL-13 in human peripheral blood CD4 T cells. We tested whether the Th2 calcineurin-independent (CsA-resistant) prostaglandin D-CrTh2 pathway (38) was responsible for CsA-resistant IL-13 production in the CD4γ13 T cell clones. It was not, and the microarray showed diminished CrTh2 mRNA transcripts in CD4γ13 compared to multifunctional Th1 clones (Table 2). These data suggest that CD4γ13 T cells have a TCR signaling pathway that regulates IL-13 production independent of calcineurin/nuclear factor of activated T cells (NFAT) activation.

The differentiation/cytokine polarization of Chlamydia-specific IFN-γ-producing CD4 T cells generated during clearance of Chlamydia genital tract infections appears to be based on the transcription factors Gata3 and Eomes and, usually but not always, Tbet. A previous study showed few Gata3+ CD4 T cells in spleen and lymph nodes during C. muridarum infection without investigation of genital tract tissue (41). A human study showed CD4 Gata3+ T cells in presumed memory lymphocyte clusters in endometrial biopsies from C. trachomatis-infected women but not uninfected controls (45). In isolation, two CD4γ13 T cell clones and four multifunctional CD4 T cell clone controls are not sufficient to draw definitive conclusions about T cell biology. However, in combination with published cytokine regulation data, it is reasonable to extrapolate to preliminary conclusions. Our results are consistent with the existing paradigm that Tbet (Th1) must be downregulated for a CD4γ13 T cell clone to produce IL-4 (Th2): e.g., sBT16-8. More importantly we saw that Fhl2 was CD4γ13 associated at the transcript level (microarray) and protein level (Western blot). Because Fhl2 knockout mice have a deficit in IL-13 production (46), it is reasonable to postulate that Fhl2 is the transcription factor that denotes a CD4γ13 T cell's ability to produce IL-13, and Fhl2 identifies an ideal candidate pathway for calcineurin-independent IL-13 production.

Although not conventionally quantifiable, we empirically discovered that immune B cell antigen presentation and exogenous TGF-β1 were necessary to recover CD4γ13 T cells and maintain their IL-13 production ex vivo (Fig. S3). Absent TGF-β1, IL-13 production seen in early passages of polyclonal T cells faded with serial passage suggesting that in vivo CD4γ13 memory T cells reside in a microenvironment with active TGF-β1. Epithelial cells make latent TGF-β constitutively, and TGF-β in the latent form is abundant in mucosal tissues. The CD4γ13 T cell molecular fingerprint (microarray) includes genes relevant to TGF-β biology, including Lrrc16a, coding for the binding receptor for the latent TGF-β complex, Bace2 and Cpa3, coding for proteases that potentially process latent TGF-β into the active form for use in an autocrine/paracrine fashion, and Avr2a, coding for the receptor for activins that share some TGF-β signaling pathways. These genes may provide new insights into the unique biology of immune B cells and memory lymphocyte clusters within the genital tract.

One of the most important findings in our present investigation is that CD4γ13 T cell clone sBT13-7 completely protected oviducts from pathology in adoptive transfer-C. muridarum challenge experiments, as did to a lesser extent the multifunctional Th1 clone 4uvmo-3. Those results link multifunctional Th1 and CD4γ13 T cells to protective immunity, with the interesting difference being that CD4γ13 sBT13-7 had a Trm mRNA fingerprint (Klf2neg and S1pr1neg) compared to Th1 4uvmo-3 (Klf2pos and S1PR1pos). The CD4γ13 phenotype in and of itself was not sufficient for genital tract protection as the CD4γ13 sBT16-8 clone was not protective; sBT16-8 was also relatively ineffective at terminating Chlamydia replication in epithelial cells in vitro. The mechanism of oviduct protection for 4uvmo-3 and sBT13-7 was not accelerated bacterial clearance as measured in the lower genital tract: 4uvmo-3 mice cleared at same rate as naive mice, while sBT13-7 mice had greater bacterial shedding at late time points, a result that may eventually provide insights into why IL-13 knockout mice clear infections faster than wild-type mice (40). At the level of individual T cell clone-mediated immunity, in wild-type mice with normal immune systems, this study shows that protection from pathology and bacterial shedding can be dissociated. This dissociation has been previously demonstrated in mouse models with plasmid-deficient C. muridarum (47) and knockouts of TLR2 (48) and IL-1b (49). sBT13-7 protection from immunopathology implies a regulatory mechanism that influenced the naive wild-type adaptive immune response, perhaps moderating the primary CD8 T cell response associated with immunopathology (20, 22, 23). Both CD4γ13 T cell clones have enhanced mRNA transcript levels for several regulatory T cell (Treg)-associated genes, including Nrn1, Ctse, and Lrrc32; however, they are not Tregs as they produced IL-2 upon activation and neither had an mRNA signal for Foxp3. In our opinion, it is unlikely likely that Treg or induced Treg (iTreg) mechanisms fully account for protection from pathology mediated by sBT13-7. We suspect sBT13-7 somehow reduced neutrophil recruitment and/or promoted a more beneficial healing response within the TNF-α–IL-13–TGF-β axis (50, 51). Recent work by Li et al. demonstrated that CCR7 homing contributes to the paucity of T lymphocytes in the naive murine female genital tract, presumably by lymph node sequestration. Naive CCR7 knockout mice have an aberrant immune architecture with many more T cells localized in genital tract tissue and cleared C. muridarum more rapidly and with less acute inflammation than did wild-type mice (52). In our study, adoptive transfer of the CD4γ13 T cell clone sBT13-7 into wild-type mice with their usual paucity of immune architecture and T cells prevented oviduct immunopathology. The CD4γ13 T cell clones had enhanced CCR8 (5- to 12-fold higher [Table 1]) with reduced CCR7 mRNA transcripts (Table 3 and GEO data) compared to the four non-CD4γ13 clones. Therefore, it is possible that CCR8, associated with skin homing (53), plays a role in c-MLC. While the mechanism of sBT13-7 protection remains to be determined, during primary genital tract infections in wild-type mice, it is reasonable to postulate that “how” is at least as or more important than “how fast” Chlamydia is cleared, with implications for assessment of future vaccines.

It is unlikely that IL-13 directly participates in the physical termination of Chlamydia cells replicating in reproductive tract epithelium, as we have shown that IL-13 modestly enhances C. muridarum replication in an upper reproductive tract epithelial cell line (36), and others have shown that IL-13 knockout mice show accelerated bacterial clearance from the genital tract (40). Instead we postulate that IL-13 is a biomarker for a CD4 Trm subset capable of preventing immunopathology during clearance of genital tract infections and that small numbers of CD4γ13 T cells in circulation are the source of EB-stimulated PBMC IL-13 production that predicted resistance to C. trachomatis infection in the study by Cohen et al. of Kenyan female sex workers. We anticipate that studies of CD4γ13 T cells in the mouse model will provide the tools necessary to test those hypotheses in humans.

Recently it has been proposed that protective/healing Th2 immunity explains how the majority of humans clear Chlamydia genital tract infections without fertility-limiting immunopathology based on Gata3-centered data (45, 54–56). That Th2 conclusion is consistent with the existing Th1/2 paradigm and reasonable as long as Gata3 is tightly associated with the Th2 phenotype. However, our data, generated in a productive Chlamydia genital tract infection model that reproduces human pathology, including infertility and hydrosalpinx, shows that Chlamydia-specific CD4 T cell clones universally expressed Gata3 and produced IFN-γ upon activation—even a CD4 clone with little or no Tbet expression—violating the basic mutual exclusivity tenets of the Th1/2 paradigm.

MATERIALS AND METHODS

Mice.Four- to 5-week-old female C57BL/6 mice were purchased from Harlan Labs (Indianapolis, IN) and Jackson Laboratory (Bar Harbor, MA). Mice were housed in Indiana University Purdue University—Indianapolis (IUPUI) and Yale University specific-pathogen-free (SPF) facilities. The Institutional Animal Care and Utilization Committees at Indiana University, Yale University, and University of British Columbia approved all experimental protocols.

Cells and bacteria.McCoy fibroblasts were cultured as previously described (37). Mycoplasma-free Chlamydia muridarum Nigg, previously known as C. trachomatis strain mouse pneumonitis (MoPn), was grown in McCoy cells as previously described (57). Soluble Chlamydia antigen (infected cell lysate depleted of EBs by centrifugation) was prepared as previously described (36), aliquoted, and stored at −80°C.

Chlamydia-specific CD4 T cells.Conventional multifunction Chlamydia-specific Th1 clone 4uvmo-3 was previously described (37). For the new B cell APC-derived T cells, C57BL/6 mice were treated with 2.5 mg of medroxyprogesterone (Pfizer) delivered subcutaneously and then infected 7 days later with 5 × 104 inclusion-forming units (IFU) of C. muridarum. Mice that cleared infection (≥6 weeks postinfection) were used as the source of immune B and T cells. Initial Chlamydia-specific immune B cell-derived polyclonal T cell populations and clones were derived as follows. Splenocytes were harvested from immune mice. Immune B cells were purified from a portion of those splenocytes by “untouched” magnetic bead separation (Miltenyi Biotech). Immune B cells were pulsed with UV-MoPn (3.5 × 106 IFU equivalents per 7.5 × 105 B cells suspended at 7.5 × 106/ml [∼5 IFU/cell]) or soluble antigen (7.5 μl per 7.5 × 105 B cells suspended at 7.5 × 106/ml) for 1 h at 37°C. Antigen-pulsed immune B cells were transferred to 7.5 ml of RPMI complete medium and pelleted, medium containing antigen was removed, and then the cells were washed two more times with 7.5 ml of medium (∼400,000-fold) to eliminate all non-cell-bound or internalized Chlamydia antigen; the purpose of extended washing was to ensure that antigen presentation was limited to immune B cells. Primary stimulation wells were set up with 2.5 × 106 immune splenocytes plus 7.5 × 105 antigen-pulsed immune B cells in 0.75 ml RPMI complete medium supplemented with recombinant cytokines and conditioned medium as previously described (36); later T cell derivations during the course of the project included addition of 5 ng/ml recombinant murine TGF-β1 to the medium. Limiting dilution cloning was done in medium supplemented with recombinant cytokines/conditioned medium with 10 to 20 ng/ml TGF-β1. Restimulation/maintenance of T cell clones was done weekly in 48-well plates by adding 100,000 to 200,000 T cell clone cells to 1.5 × 106 irradiated naive splenocytes and 7.5 × 105 irradiated relevant antigen-pulsed, washed immune B cells as feeders in medium supplemented with recombinant cytokines/conditioned medium, including 2.5 to 10 ng/ml TGF-β1. Recombinant mouse cytokines were purchased from the same vendor (R&D Systems; Minneapolis, MN), except for TGF-β1 (Ebioscience, San Diego, CA).

Flow cytometry and intracellular cytokine staining.For B cell staining, single-cell suspensions of genital tracts pooled from four mice per experimental group were surface stained using anti-mouse B220 (RA3-6B2 coupled to fluorescein isothiocyanate [FITC]; BD Pharmingen) as well as with the viability dye aqua fluorescent reactive dye (L34957; Molecular Probes), followed by intracellular staining using anti-mouse CD79a (24C2.5 coupled to eFluor660; eBioscience). The experiment was repeated 1 or 2 times for individual experimental groups.

T cell surface phenotypes were determined using antibodies to CD4 (GK1.5 coupled to phycoerythrin [PE]) and CD8a (53-6.7 coupled to FITC). T cells were stained for 20 min at 4°C with 1 μg per 1 million T cells in RPMI complete medium with 10% fetal bovine serum (FBS), fixed with 1% paraformaldehyde, and analyzed by flow cytometry (BD Facscalibur or LSRII). For intracellular staining for IL-13 (ebio13A coupled to PE) and IFN-γ (XMG1.2 coupled to allophycocyanin), T cells were activated for 5 h in a cocktail of phorbol 12-myristate 13-acetate (PMA), ionomycin, brefeldin A, and monensin (cell stimulation cocktail; Ebioscience), stained for CD8a, and then fixed and permeabilized (Fix/Perm buffer; Ebioscience), stained for IL-13–PE/IFN-γ–allophycocyanin or control antibody (eBRG1-PE/IFN-γ–allophycocyanin) in the presence of 2 mg/ml donkey IgG (Jackson ImmunoResearch) for 30 min at room temperature, washed, suspended in 1% paraformaldehyde, and analyzed. All of the T cell populations are >90% CD4 T cells; negative staining based on CD8a was chosen because PMA-ionomycin activation resulted in shedding of cell surface CD4 and diminished CD4 staining; CD8a staining was not affected (data not shown).

Cytokine ELISAs and signaling reagents.2.5 × 104 Ficoll-Hypaque-purified T cell clones (5 × 104 purified T cells for IL-4 determination) cultured overnight in RPMI medium with 3 ng/ml IL-7 were activated in 96-well tissue culture plates by immobilized anti-CD3 monoclonal antibody 145-2c11, at 0.5 μg/ml in phosphate-buffered saline (PBS) overnight at 4°C (washed once), in RPMI medium containing 1 ng/ml recombinant murine IL-7 (R&D Systems, Minneapolis, MN) for 20 h. Relative levels of IL-2, IFN-γ, IL-13, IL-10, TNF-α, IL-17, IL-22, IL-4, and IL-5 in culture supernatants were determined by ELISA using capture and biotinylated monoclonal antibody pairs with recombinant murine standards according to the manufacturer's protocols: for IL-2, JES6-1A12/Jes6-5H4; for IFN-γ ELISA, XMG1.2; (Pierce-Thermo Fisher, Rockford IL); for IL-13 ELISA, eBio13A/eBio1316H (Ebioscience); for IL-10, Jess-16E3/Jess-2A5; for TNF-α, TN3-19.12/rabbit anti-mouse/rat polyclonal (BD Biosciences); for IL-17, 17CK15A5/17B7 (Ebioscience); for IL-22, polyclonal 5164 (Biolegend); for IL-5, TRFK5/TRFK4; and for IL-4, 11b11/BVD6-24g2 (Ebioscience). Detection was accomplished with streptavidin-horseradish peroxidase (HRP) (BD Biosciences) and tetramethylbenzidine (TMB) substrate (Sigma Chemical Co).

Cyclosporine was purchased from Sigma and dissolved in ethanol. CrTh2 inhibitors I [(4-chloro-2-((2-methyl-5-(propylsulfonyl)phenyl)ethynyl)phenoxy)acetic acid] and II [(R)-(5-chloro-1′-(5-chloro-2-fluorobenzyl)-2,2′,5′-trioxospiro(indole-3,3→-pyrrolidin)-1(2H)-yl)acetic acid] were purchased from EMD Millipore (Temecula, CA) and dissolved in dimethyl sulfoxide (DMSO).

Redirected lysis.Redirected lysis was performed as described by Leo et al. (58). 10,000 P815 cells (ATCC TIB-64; American Type Culture Collection, Manassas, VA), from a mastocytoma cell line expressing Fc receptors (FcRs), were incubated with 10,000 CD4 T cells in the presence of 0.5 μg/ml anti-CD3e (clone145-2c11, NA/LE; BD Biosciences, San Jose, CA) in 96-well V-bottom plates, spun for 1 min at 300 × g, and then incubated for 4 h. Killing was quantified using a nonradioactive cytotoxicity assay measuring release of lactate dehydrogenase activity in culture supernatant (cyto 96; Promega, Madison, WI) following the manufacturer's protocol. The lysis assays were done using RPMI complete medium with 1% heat-inactivated serum (68°C for 30 min to inactivate lactate dehydrogenase activity present in FBS). The percentage of specific lysis was calculated as [(experimental release of T cells + P815 + anti-CD3) − (spontaneous release of T cells + P815 without antibody)/(maximal release by Triton X-100 treatment of P815)] × 100.

Adoptive transfer and genital tract infections.T cells were purified with Ficoll-Hypaque (Histopaque 1083; Sigma Chemical Co, St. Louis, MO) on day 5 of the culture cycle and maintained in RPMI complete medium with 3 ng/ml murine recombinant IL-7 for 2 days prior to adoptive transfer. One week prior to infection, mice were treated with 2.5 mg of medroxyprogesterone (Pfizer) delivered subcutaneously. Six days later, 1 × 106 T cell clone cells were adoptively transferred via retro-orbital injection into fully anesthetized mice; controls were injected with an equivalent volume of phosphate-buffered saline (PBS). The day following adoptive transfer, lightly anesthetized mice were infected vaginally with 5 × 104 IFU of C. muridarum in 10 μl of SPG buffer (10 mM sodium phosphate [8 mM Na2HPO4-2 mM NaH2PO4], 220 mM sucrose, 0.50 mM l-glutamic acid). Mice were serially swabbed through day 30 postinfection, and IFU were determined on McCoy cells to quantify bacterial shedding. On day 56 postinfection, the mice were killed and genital tracts scored for pathology as previously described (39). Briefly, each mouse genital tract has 2 uteri and 2 oviducts; one point is assigned for macroscopic (visible) thinning to dilatation of each site for a maximum score of 4 per mouse. Scoring is done in situ; the genital tracts are then excised and photographed for a digital record (qualitative data). The adoptive transfer experiments were aggregated for Chi-square analysis.

Gene expression microarray analysis.For the “resting” phenotype microarrays, Chlamydia-specific CD4 T cell clones 4uvmo-3, BT12-7, BT12-17, sBT13-11, sBT13-7, and sBT16-8 were purified by Ficoll-Hypaque at the end of their usual 7-day culture cycle and then maintained in RPMI complete medium with 3 ng/ml IL-7 for 48 h without antigen stimulation. Total RNA was isolated from each T cell clone using a protocol that included a genomic DNA removal step (G-eliminator; RNeasy; Qiagen, Valencia, CA). RNA isolation under these culture conditions was repeated 4 times (independent experiments) for each clone to minimize false discovery. With assistance from The Indiana University Center for Medical Genomics, gene expression patterns were analyzed using Affymetrix Clariom S mouse arrays, which analyze >20,000 well-annotated genes. Samples were labeled using the standard Affymetrix protocol for the Affymetrix WT Plus kit using 100 ng of total RNA. Individual labeled samples were hybridized to the Mouse Clariom S GeneChips for 17 h and then washed, stained, and scanned with the standard protocol using Affymetrix GeneChip Command Console software (AGCC) to generate data (CEL files). Arrays were visually scanned for abnormalities or defects. CEL files were imported into Partek Genomics Suite (Partek, Inc., St. Louis, MO).

Western blots.Polyclonal rabbit antiserum specific for Eomes (Thermo Fisher Scientific catalog no. 720202), Tbet/Gata3/Fhl2 (Proteintech catalog no. 13700-1-AP/10417-1-AP/21619-1-AP), and HRP-coupled monoclonal antibody to β-actin (Sigma-Aldrich catalog no. A3854) were obtained from commercial vendors. Ten micrograms of whole-cell lysate protein was run on 4 to 12% gradient gels and transferred to nitrocellulose using a dry blotting system (iBlot; Thermo Fisher). Membranes were rinsed in Tris-buffered saline with Tween 20 (TBST) and blocked with 5% nonfat milk in TBST. Rabbit antisera were detected with a rabbit-specific chemiluminescent kit (Thermo Fisher catalog no. WB7106). Detection was performed with a commercial chemiluminescent substrate (Thermo Fisher catalog no. 34080).

Statistical methods.As indicated in each figure legend, aggregated data were analyzed by two-sample Student's t test using Origin 8.0 software. Exceptions were Fig. 3B and 4 (analysis of variance [ANOVA]) and Fig. 6 (Dunnett's test), which were analyzed with R software, and Fig. 6C, which underwent chi-square analysis.

Accession number(s).The microarray data presented here are available in the Gene Expression Omnibus database under accession no. GSE104743.

ACKNOWLEDGMENTS

This research was supported by NIH/NIAID grant R01AI113103.

Critical assistance with microarray data analysis was provided by Jeanette McClintick and the Indiana University Center for Medical Genomics.

We have no conflicts of interest related to the content of this article.

FOOTNOTES

    • Received 28 August 2017.
    • Returned for modification 21 September 2017.
    • Accepted 10 November 2017.
    • Accepted manuscript posted online 20 November 2017.
  • Supplemental material for this article may be found at https://doi.org/10.1128/IAI.00614-17.

  • Copyright © 2018 American Society for Microbiology.

All Rights Reserved.

REFERENCES

  1. 1.↵
    1. Brunham RC,
    2. Pourbohloul B,
    3. Mak S,
    4. White R,
    5. Rekart ML
    . 2005. The unexpected impact of a Chlamydia trachomatis infection control program on susceptibility to reinfection. J Infect Dis 192:1836–1844. doi:10.1086/497341.
    OpenUrlCrossRefPubMedWeb of Science
  2. 2.↵
    1. Brunham RC,
    2. Rekart ML
    . 2008. The arrested immunity hypothesis and the epidemiology of chlamydia control. Sex Transm Dis 35:53–54. doi:10.1097/OLQ.0b013e31815e41a3.
    OpenUrlCrossRefPubMedWeb of Science
  3. 3.↵
    1. Su H,
    2. Morrison R,
    3. Messer R,
    4. Whitmire W,
    5. Hughes S,
    6. Caldwell HD
    . 1999. The effect of doxycycline treatment on the development of protective immunity in a murine model of chlamydial genital infection. J Infect Dis 180:1252–1258. doi:10.1086/315046.
    OpenUrlCrossRefPubMedWeb of Science
  4. 4.↵
    1. Molano M,
    2. Meijer CJ,
    3. Weiderpass E,
    4. Arslan A,
    5. Posso H,
    6. Franceschi S,
    7. Ronderos M,
    8. Munoz N,
    9. van den Brule AJ
    . 2005. The natural course of Chlamydia trachomatis infection in asymptomatic Colombian women: a 5-year follow-up study. J Infect Dis 191:907–916. doi:10.1086/428287.
    OpenUrlCrossRefPubMedWeb of Science
  5. 5.↵
    1. van den Brule AJ,
    2. Munk C,
    3. Winther JF,
    4. Kjaer SK,
    5. Jorgensen HO,
    6. Meijer CJ,
    7. Morre SA
    . 2002. Prevalence and persistence of asymptomatic Chlamydia trachomatis infections in urine specimens from Danish male military recruits. Int J STD AIDS 13(Suppl 2):S19–S22. doi:10.1258/095646202762226100.
    OpenUrlCrossRef
  6. 6.↵
    1. Morre SA,
    2. van den Brule AJ,
    3. Rozendaal L,
    4. Boeke AJ,
    5. Voorhorst FJ,
    6. de Blok S,
    7. Meijer CJ
    . 2002. The natural course of asymptomatic Chlamydia trachomatis infections: 45% clearance and no development of clinical PID after one-year follow-up. Int J STD AIDS 13(Suppl 2):S12–S18. doi:10.1258/095646202762226092.
    OpenUrlCrossRef
  7. 7.↵
    1. Geisler WM,
    2. Lensing SY,
    3. Press CG,
    4. Hook EW, III
    . 2013. Spontaneous resolution of genital Chlamydia trachomatis infection in women and protection from reinfection. J Infect Dis 207:1850–1856. doi:10.1093/infdis/jit094.
    OpenUrlCrossRefPubMed
  8. 8.↵
    1. Yu H,
    2. Karunakaran KP,
    3. Jiang X,
    4. Shen C,
    5. Andersen P,
    6. Brunham RC
    . 2012. Chlamydia muridarum T cell antigens and adjuvants that induce protective immunity in mice. Infect Immun 80:1510–1518. doi:10.1128/IAI.06338-11.
    OpenUrlAbstract/FREE Full Text
  9. 9.↵
    1. Farris CM,
    2. Morrison SG,
    3. Morrison RP
    . 2010. CD4+ T cells and antibody are required for optimal major outer membrane protein vaccine-induced immunity to Chlamydia muridarum genital infection. Infect Immun 78:4374–4383. doi:10.1128/IAI.00622-10.
    OpenUrlAbstract/FREE Full Text
  10. 10.↵
    1. Olsen AW,
    2. Follmann F,
    3. Erneholm K,
    4. Rosenkrands I,
    5. Andersen P
    . 2015. Protection against Chlamydia trachomatis infection and upper genital tract pathological changes by vaccine-promoted neutralizing antibodies directed to the VD4 of the major outer membrane protein. J Infect Dis 212:978–989. doi:10.1093/infdis/jiv137.
    OpenUrlCrossRefPubMed
  11. 11.↵
    1. Pal S,
    2. Peterson EM,
    3. de la Maza LM
    . 2005. Vaccination with the Chlamydia trachomatis major outer membrane protein can elicit an immune response as protective as that resulting from inoculation with live bacteria. Infect Immun 73:8153–8160. doi:10.1128/IAI.73.12.8153-8160.2005.
    OpenUrlAbstract/FREE Full Text
  12. 12.↵
    1. Tifrea DF,
    2. Pal S,
    3. Popot JL,
    4. Cocco MJ,
    5. de la Maza LM
    . 2014. Increased immunoaccessibility of MOMP epitopes in a vaccine formulated with amphipols may account for the very robust protection elicited against a vaginal challenge with Chlamydia muridarum. J Immunol 192:5201–5213. doi:10.4049/jimmunol.1303392.
    OpenUrlAbstract/FREE Full Text
  13. 13.↵
    1. Morrison SG,
    2. Morrison RP
    . 2005. A predominant role for antibody in acquired immunity to chlamydial genital tract reinfection. J Immunol 175:7536–7542. doi:10.4049/jimmunol.175.11.7536.
    OpenUrlAbstract/FREE Full Text
  14. 14.↵
    1. Su H,
    2. Feilzer K,
    3. Caldwell HD,
    4. Morrison RP
    . 1997. Chlamydia trachomatis genital tract infection of antibody-deficient gene knockout mice. Infect Immun 65:1993–1999.
    OpenUrlAbstract/FREE Full Text
  15. 15.↵
    1. Su H,
    2. Parnell M,
    3. Caldwell HD
    . 1995. Protective efficacy of a parenterally administered MOMP-derived synthetic oligopeptide vaccine in a murine model of Chlamydia trachomatis genital tract infection: serum neutralizing IgG antibodies do not protect against chlamydial genital tract infection. Vaccine 13:1023–1032. doi:10.1016/0264-410X(95)00017-U.
    OpenUrlCrossRefPubMedWeb of Science
  16. 16.↵
    1. Brunham RC,
    2. Kuo CC,
    3. Cles L,
    4. Holmes KK
    . 1983. Correlation of host immune response with quantitative recovery of Chlamydia trachomatis from the human endocervix. Infect Immun 39:1491–1494.
    OpenUrlAbstract/FREE Full Text
  17. 17.↵
    1. Arno JN,
    2. Katz BP,
    3. McBride R,
    4. Carty GA,
    5. Batteiger BE,
    6. Caine VA,
    7. Jones RB
    . 1994. Age and clinical immunity to infections with Chlamydia trachomatis. Sex Transm Dis 21:47–52. doi:10.1097/00007435-199401000-00010.
    OpenUrlCrossRefPubMedWeb of Science
  18. 18.↵
    1. Cohen CR,
    2. Koochesfahani KM,
    3. Meier AS,
    4. Shen C,
    5. Karunakaran K,
    6. Ondondo B,
    7. Kinyari T,
    8. Mugo NR,
    9. Nguti R,
    10. Brunham RC
    . 2005. Immunoepidemiologic profile of Chlamydia trachomatis infection: importance of heat-shock protein 60 and interferon-gamma. J Infect Dis 192:591–599. doi:10.1086/432070.
    OpenUrlCrossRefPubMedWeb of Science
  19. 19.↵
    1. Ness RB,
    2. Soper DE,
    3. Richter HE,
    4. Randall H,
    5. Peipert JF,
    6. Nelson DB,
    7. Schubeck D,
    8. McNeeley SG,
    9. Trout W,
    10. Bass DC,
    11. Hutchison K,
    12. Kip K,
    13. Brunham RC
    . 2008. Chlamydia antibodies, chlamydia heat shock protein, and adverse sequelae after pelvic inflammatory disease: the PID Evaluation and Clinical Health (PEACH) Study. Sex Transm Dis 35:129–135. doi:10.1097/OLQ.0b013e3181557c25.
    OpenUrlCrossRefPubMedWeb of Science
  20. 20.↵
    1. Igietseme JU,
    2. He Q,
    3. Joseph K,
    4. Eko FO,
    5. Lyn D,
    6. Ananaba G,
    7. Campbell A,
    8. Bandea C,
    9. Black CM
    . 2009. Role of T lymphocytes in the pathogenesis of Chlamydia disease. J Infect Dis 200:926–934. doi:10.1086/605411.
    OpenUrlCrossRefPubMed
  21. 21.↵
    1. Manam S,
    2. Nicholson BJ,
    3. Murthy AK
    . 2013. OT-1 mice display minimal upper genital tract pathology following primary intravaginal Chlamydia muridarum infection. Pathog Dis 67:221–224. doi:10.1111/2049-632X.12032.
    OpenUrlCrossRefPubMed
  22. 22.↵
    1. Murthy AK,
    2. Li W,
    3. Chaganty BK,
    4. Kamalakaran S,
    5. Guentzel MN,
    6. Seshu J,
    7. Forsthuber TG,
    8. Zhong G,
    9. Arulanandam BP
    . 2011. Tumor necrosis factor alpha production from CD8+ T cells mediates oviduct pathological sequelae following primary genital Chlamydia muridarum infection. Infect Immun 79:2928–2935. doi:10.1128/IAI.05022-11.
    OpenUrlAbstract/FREE Full Text
  23. 23.↵
    1. Vlcek KR,
    2. Li W,
    3. Manam S,
    4. Zanotti B,
    5. Nicholson BJ,
    6. Ramsey KH,
    7. Murthy AK
    . 2016. The contribution of Chlamydia-specific CD8(+) T cells to upper genital tract pathology. Immunol Cell Biol 94:208–212. doi:10.1038/icb.2015.74.
    OpenUrlCrossRef
  24. 24.↵
    1. Olivares-Zavaleta N,
    2. Whitmire WM,
    3. Kari L,
    4. Sturdevant GL,
    5. Caldwell HD
    . 2014. CD8+ T cells define an unexpected role in live-attenuated vaccine protective immunity against Chlamydia trachomatis infection in macaques. J Immunol 192:4648–4654. doi:10.4049/jimmunol.1400120.
    OpenUrlAbstract/FREE Full Text
  25. 25.↵
    1. Picard MD,
    2. Bodmer JL,
    3. Gierahn TM,
    4. Lee A,
    5. Price J,
    6. Cohane K,
    7. Clemens V,
    8. DeVault VL,
    9. Gurok G,
    10. Kohberger R,
    11. Higgins DE,
    12. Siber GR,
    13. Flechtner JB,
    14. Geisler WM
    . 2015. Resolution of Chlamydia trachomatis infection is associated with a distinct T cell response profile. Clin Vaccine Immunol 22:1206–1218. doi:10.1128/CVI.00247-15.
    OpenUrlAbstract/FREE Full Text
  26. 26.↵
    1. Morrison RP,
    2. Caldwell HD
    . 2002. Immunity to murine chlamydial genital infection. Infect Immun 70:2741–2751. doi:10.1128/IAI.70.6.2741-2751.2002.
    OpenUrlFREE Full Text
  27. 27.↵
    1. de la Maza LM,
    2. Zhong G,
    3. Brunham RC
    . 2017. Update in Chlamydia trachomatis vaccinology. Clin Vaccine Immunol 24:e00543-16. doi:10.1128/CVI.00543-16.
    OpenUrlCrossRef
  28. 28.↵
    1. Gondek DC,
    2. Roan NR,
    3. Starnbach MN
    . 2009. T cell responses in the absence of IFN-gamma exacerbate uterine infection with Chlamydia trachomatis. J Immunol 183:1313–1319. doi:10.4049/jimmunol.0900295.
    OpenUrlAbstract/FREE Full Text
  29. 29.↵
    1. Hawkins RA,
    2. Rank RG,
    3. Kelly KA
    . 2002. A Chlamydia trachomatis-specific Th2 clone does not provide protection against a genital infection and displays reduced trafficking to the infected genital mucosa. Infect Immun 70:5132–5139. doi:10.1128/IAI.70.9.5132-5139.2002.
    OpenUrlAbstract/FREE Full Text
  30. 30.↵
    1. Johnson RM,
    2. Brunham RC
    . 2016. Tissue-resident T cells as the central paradigm of Chlamydia immunity. Infect Immun 84:868–873. doi:10.1128/IAI.01378-15.
    OpenUrlAbstract/FREE Full Text
  31. 31.↵
    1. Morrison SG,
    2. Morrison RP
    . 2000. In situ analysis of the evolution of the primary immune response in murine Chlamydia trachomatis genital tract infection. Infect Immun 68:2870–2879. doi:10.1128/IAI.68.5.2870-2879.2000.
    OpenUrlAbstract/FREE Full Text
  32. 32.↵
    1. Kelly KA,
    2. Rank RG
    . 1997. Identification of homing receptors that mediate the recruitment of CD4 T cells to the genital tract following intravaginal infection with Chlamydia trachomatis. Infect Immun 65:5198–5208.
    OpenUrlAbstract/FREE Full Text
  33. 33.↵
    1. Kiviat NB,
    2. Paavonen JA,
    3. Wolner-Hanssen P,
    4. Critchlow CW,
    5. Stamm WE,
    6. Douglas J,
    7. Eschenbach DA,
    8. Corey LA,
    9. Holmes KK
    . 1990. Histopathology of endocervical infection caused by Chlamydia trachomatis, herpes simplex virus, Trichomonas vaginalis, and Neisseria gonorrhoeae. Hum Pathol 21:831–837. doi:10.1016/0046-8177(90)90052-7.
    OpenUrlCrossRefPubMedWeb of Science
  34. 34.↵
    1. Dustin LB,
    2. Bullock ED,
    3. Hamada Y,
    4. Azuma T,
    5. Loh DY
    . 1995. Antigen-driven differentiation of naive Ig-transgenic B cells in vitro. J Immunol 154:4936–4949.
    OpenUrlAbstract
  35. 35.↵
    1. Rock KL,
    2. Benacerraf B,
    3. Abbas AK
    . 1984. Antigen presentation by hapten-specific B lymphocytes. I. Role of surface immunoglobulin receptors. J Exp Med 160:1102–1113.
    OpenUrlAbstract/FREE Full Text
  36. 36.↵
    1. Johnson RM,
    2. Kerr MS,
    3. Slaven JE
    . 2014. An atypical CD8 T-cell response to Chlamydia muridarum genital tract infections includes T cells that produce interleukin-13. Immunology 142:248–257. doi:10.1111/imm.12248.
    OpenUrlCrossRefPubMedWeb of Science
  37. 37.↵
    1. Jayarapu K,
    2. Kerr MS,
    3. Katschke A,
    4. Johnson RM
    . 2009. Chlamydia muridarum-specific CD4 T-cell clones recognize infected reproductive tract epithelial cells in an interferon-dependent fashion. Infect Immun 77:4469–4479. doi:10.1128/IAI.00491-09.
    OpenUrlAbstract/FREE Full Text
  38. 38.↵
    1. Xue L,
    2. Gyles SL,
    3. Wettey FR,
    4. Gazi L,
    5. Townsend E,
    6. Hunter MG,
    7. Pettipher R
    . 2005. Prostaglandin D2 causes preferential induction of proinflammatory Th2 cytokine production through an action on chemoattractant receptor-like molecule expressed on Th2 cells. J Immunol 175:6531–6536. doi:10.4049/jimmunol.175.10.6531.
    OpenUrlAbstract/FREE Full Text
  39. 39.↵
    1. Johnson RM,
    2. Kerr MS,
    3. Slaven JE
    . 2012. Plac8-dependent and inducible NO synthase-dependent mechanisms clear Chlamydia muridarum infections from the genital tract. J Immunol 188:1896–1904. doi:10.4049/jimmunol.1102764.
    OpenUrlAbstract/FREE Full Text
  40. 40.↵
    1. Asquith KL,
    2. Horvat JC,
    3. Kaiko GE,
    4. Carey AJ,
    5. Beagley KW,
    6. Hansbro PM,
    7. Foster PS
    . 2011. Interleukin-13 promotes susceptibility to chlamydial infection of the respiratory and genital tracts. PLoS Pathog 7:e1001339. doi:10.1371/journal.ppat.1001339.
    OpenUrlCrossRefPubMed
  41. 41.↵
    1. Li LX,
    2. McSorley SJ
    . 2013. B cells enhance antigen-specific CD4 T cell priming and prevent bacteria dissemination following Chlamydia muridarum genital tract infection. PLoS Pathog 9:e1003707. doi:10.1371/journal.ppat.1003707.
    OpenUrlCrossRefPubMed
  42. 42.↵
    1. Li LX,
    2. McSorley SJ
    . 2015. A re-evaluation of the role of B cells in protective immunity to Chlamydia infection. Immunol Lett 164:88–93. doi:10.1016/j.imlet.2015.02.004.
    OpenUrlCrossRefPubMed
  43. 43.↵
    1. Mackay LK,
    2. Kallies A
    . 2017. Transcriptional regulation of tissue-resident lymphocytes. Trends Immunol 38:94–103. doi:10.1016/j.it.2016.11.004.
    OpenUrlCrossRef
  44. 44.↵
    1. Pahl A,
    2. Zhang M,
    3. Kuss H,
    4. Szelenyi I,
    5. Brune K
    . 2002. Regulation of IL-13 synthesis in human lymphocytes: implications for asthma therapy. Br J Pharmacol 135:1915–1926. doi:10.1038/sj.bjp.0704656.
    OpenUrlCrossRefPubMedWeb of Science
  45. 45.↵
    1. Vicetti Miguel RD,
    2. Harvey SA,
    3. LaFramboise WA,
    4. Reighard SD,
    5. Matthews DB,
    6. Cherpes TL
    . 2013. Human female genital tract infection by the obligate intracellular bacterium Chlamydia trachomatis elicits robust type 2 immunity. PLoS One 8:e58565. doi:10.1371/journal.pone.0058565.
    OpenUrlCrossRefPubMed
  46. 46.↵
    1. Kurakula K,
    2. Vos M,
    3. Logiantara A,
    4. Roelofs JJ,
    5. Nieuwenhuis MA,
    6. Koppelman GH,
    7. Postma DS,
    8. Brandsma CA,
    9. Sin DD,
    10. Bosse Y,
    11. Nickle DC,
    12. van Rijt LS,
    13. de Vries CJ
    . 2015. Deficiency of FHL2 attenuates airway inflammation in mice and genetic variation associates with human bronchial hyper-responsiveness. Allergy 70:1531–1544. doi:10.1111/all.12709.
    OpenUrlCrossRef
  47. 47.↵
    1. O'Connell CM,
    2. Ingalls RR,
    3. Andrews CW, Jr,
    4. Scurlock AM,
    5. Darville T
    . 2007. Plasmid-deficient Chlamydia muridarum fail to induce immune pathology and protect against oviduct disease. J Immunol 179:4027–4034. doi:10.4049/jimmunol.179.6.4027.
    OpenUrlAbstract/FREE Full Text
  48. 48.↵
    1. Darville T,
    2. O'Neill JM,
    3. Andrews CW, Jr,
    4. Nagarajan UM,
    5. Stahl L,
    6. Ojcius DM
    . 2003. Toll-like receptor-2, but not Toll-like receptor-4, is essential for development of oviduct pathology in chlamydial genital tract infection. J Immunol 171:6187–6197. doi:10.4049/jimmunol.171.11.6187.
    OpenUrlAbstract/FREE Full Text
  49. 49.↵
    1. Prantner D,
    2. Darville T,
    3. Sikes JD,
    4. Andrews CW, Jr,
    5. Brade H,
    6. Rank RG,
    7. Nagarajan UM
    . 2009. Critical role for interleukin-1beta (IL-1beta) during Chlamydia muridarum genital infection and bacterial replication-independent secretion of IL-1beta in mouse macrophages. Infect Immun 77:5334–5346. doi:10.1128/IAI.00883-09.
    OpenUrlAbstract/FREE Full Text
  50. 50.↵
    1. Fichtner-Feigl S,
    2. Strober W,
    3. Kawakami K,
    4. Puri RK,
    5. Kitani A
    . 2006. IL-13 signaling through the IL-13alpha2 receptor is involved in induction of TGF-beta1 production and fibrosis. Nat Med 12:99–106. doi:10.1038/nm1332.
    OpenUrlCrossRefPubMedWeb of Science
  51. 51.↵
    1. Fichtner-Feigl S,
    2. Young CA,
    3. Kitani A,
    4. Geissler EK,
    5. Schlitt HJ,
    6. Strober W
    . 2008. IL-13 signaling via IL-13R alpha2 induces major downstream fibrogenic factors mediating fibrosis in chronic TNBS colitis. Gastroenterology 135:2003–2013.e7. doi:10.1053/j.gastro.2008.08.055.
    OpenUrlCrossRefPubMedWeb of Science
  52. 52.↵
    1. Li LX,
    2. Labuda JC,
    3. Imai DM,
    4. Griffey SM,
    5. McSorley SJ
    . 2017. CCR7 deficiency allows accelerated clearance of Chlamydia from the female reproductive tract. J Immunol 199:2547–2554. doi:10.4049/jimmunol.1601314.
    OpenUrlAbstract/FREE Full Text
  53. 53.↵
    1. McCully ML,
    2. Ladell K,
    3. Hakobyan S,
    4. Mansel RE,
    5. Price DA,
    6. Moser B
    . 2012. Epidermis instructs skin homing receptor expression in human T cells. Blood 120:4591–4598. doi:10.1182/blood-2012-05-433037.
    OpenUrlAbstract/FREE Full Text
  54. 54.↵
    1. Vicetti Miguel RD,
    2. Cherpes TL
    . 2012. Hypothesis: Chlamydia trachomatis infection of the female genital tract is controlled by type 2 immunity. Med Hypotheses 79:713–716. doi:10.1016/j.mehy.2012.07.032.
    OpenUrlCrossRefPubMedWeb of Science
  55. 55.↵
    1. Vicetti Miguel RD,
    2. Quispe Calla NE,
    3. Cherpes TL
    . 2017. Setting sights on Chlamydia immunity's central paradigm: can we hit a moving target? Infect Immun 85:e00129-17. doi:10.1128/IAI.00129-17.
    OpenUrlCrossRef
  56. 56.↵
    1. Vicetti Miguel RD,
    2. Quispe Calla NE,
    3. Dixon D,
    4. Foster RA,
    5. Gambotto A,
    6. Pavelko SD,
    7. Hall-Stoodley L,
    8. Cherpes TL
    . 2017. IL-4-secreting eosinophils promote endometrial stromal cell proliferation and prevent Chlamydia-induced upper genital tract damage. Proc Natl Acad Sci U S A 114:E6892–E6901. doi:10.1073/pnas.1621253114.
    OpenUrlAbstract/FREE Full Text
  57. 57.↵
    1. Johnson RM
    . 2004. Murine oviduct epithelial cell cytokine responses to Chlamydia muridarum infection include interleukin-12-p70 secretion. Infect Immun 72:3951–3960. doi:10.1128/IAI.72.7.3951-3960.2004.
    OpenUrlAbstract/FREE Full Text
  58. 58.↵
    1. Leo O,
    2. Sachs DH,
    3. Samelson LE,
    4. Foo M,
    5. Quinones R,
    6. Gress R,
    7. Bluestone JA
    . 1986. Identification of monoclonal antibodies specific for the T cell receptor complex by Fc receptor-mediated CTL lysis. J Immunol 137:3874–3880.
    OpenUrlAbstract/FREE Full Text
PreviousNext
Back to top
Download PDF
Citation Tools
B Cell Presentation of Chlamydia Antigen Selects Out Protective CD4γ13 T Cells: Implications for Genital Tract Tissue-Resident Memory Lymphocyte Clusters
Raymond M. Johnson, Hong Yu, Norma Olivares Strank, Karuna Karunakaran, Ying Zhu, Robert C. Brunham
Infection and Immunity Jan 2018, 86 (2) e00614-17; DOI: 10.1128/IAI.00614-17

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Infection and Immunity article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
B Cell Presentation of Chlamydia Antigen Selects Out Protective CD4γ13 T Cells: Implications for Genital Tract Tissue-Resident Memory Lymphocyte Clusters
(Your Name) has forwarded a page to you from Infection and Immunity
(Your Name) thought you would be interested in this article in Infection and Immunity.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
B Cell Presentation of Chlamydia Antigen Selects Out Protective CD4γ13 T Cells: Implications for Genital Tract Tissue-Resident Memory Lymphocyte Clusters
Raymond M. Johnson, Hong Yu, Norma Olivares Strank, Karuna Karunakaran, Ying Zhu, Robert C. Brunham
Infection and Immunity Jan 2018, 86 (2) e00614-17; DOI: 10.1128/IAI.00614-17
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • INTRODUCTION
    • RESULTS
    • DISCUSSION
    • MATERIALS AND METHODS
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

Chlamydia
CD4
IL-13
B cells
Trm

Related Articles

Cited By...

About

  • About IAI
  • Editor in Chief
  • Editorial Board
  • Policies
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Ethics
  • Contact Us

Follow #IAIjournal

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0019-9567; Online ISSN: 1098-5522