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Infection and Immunity
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Cellular Microbiology: Pathogen-Host Cell Molecular Interactions

Paracellular Pathway-Mediated Mycoplasma hyopneumoniae Migration across Porcine Airway Epithelial Barrier under Air-Liquid Interface Conditions

Haiyan Wang, Zhenzhen Zhang, Xing Xie, Beibei Liu, Yanna Wei, Yuan Gan, Ting Yuan, Bo Ni, Jia Wang, Lei Zhang, Qiyan Xiong, Guoqing Shao, Zhixin Feng
Guy H. Palmer, Editor
Haiyan Wang
aInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China
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Zhenzhen Zhang
aInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China
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Xing Xie
aInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China
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Beibei Liu
aInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China
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Yanna Wei
aInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China
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Yuan Gan
aInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China
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Ting Yuan
aInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China
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Bo Ni
aInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China
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Jia Wang
aInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China
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Lei Zhang
aInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China
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Qiyan Xiong
aInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China
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Guoqing Shao
aInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China
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Zhixin Feng
aInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China
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Guy H. Palmer
Washington State University
Roles: Editor
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DOI: 10.1128/IAI.00470-20
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ABSTRACT

Mycoplasma hyopneumoniae is an important respiratory pathogen of pigs that causes persistent and secondary infections. However, the mechanisms by which this occurs are unclear. In this study, we established air-liquid interface culture systems for pig bronchial epithelial cells (ALI-PBECs) that were comparable to the conditions in the native bronchus in vivo. We used this ALI-PBECs model to study the infection and migration characteristics of M. hyopneumoniae in vitro. Based on the results, we confirmed that M. hyopneumoniae was able to adhere to ALI-PBECs and disrupt mucociliary function. Importantly, M. hyopneumoniae could migrate to the basolateral chamber through the paracellular route but not the transcellular pathway, and this was achieved by reversibly disrupting tight junctions (TJs) and increasing the permeability and damaging the integrity of the epithelial barrier. We examined the migration ability of M. hyopneumoniae using an ALI-PBECs model for the first time. The disruption of the epithelial barrier allowed M. hyopneumoniae to migrate to the basolateral chamber through the paracellular route, which may be related to immune evasion, extrapulmonary dissemination, and persistent infection of M. hyopneumoniae.

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Paracellular Pathway-Mediated Mycoplasma hyopneumoniae Migration across Porcine Airway Epithelial Barrier under Air-Liquid Interface Conditions
Haiyan Wang, Zhenzhen Zhang, Xing Xie, Beibei Liu, Yanna Wei, Yuan Gan, Ting Yuan, Bo Ni, Jia Wang, Lei Zhang, Qiyan Xiong, Guoqing Shao, Zhixin Feng
Infection and Immunity Sep 2020, 88 (10) e00470-20; DOI: 10.1128/IAI.00470-20

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Paracellular Pathway-Mediated Mycoplasma hyopneumoniae Migration across Porcine Airway Epithelial Barrier under Air-Liquid Interface Conditions
Haiyan Wang, Zhenzhen Zhang, Xing Xie, Beibei Liu, Yanna Wei, Yuan Gan, Ting Yuan, Bo Ni, Jia Wang, Lei Zhang, Qiyan Xiong, Guoqing Shao, Zhixin Feng
Infection and Immunity Sep 2020, 88 (10) e00470-20; DOI: 10.1128/IAI.00470-20
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KEYWORDS

Mycoplasma hyopneumoniae
air-liquid interface
pig bronchial epithelial cells
barrier function
migration

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