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Fungal and Parasitic Infections

Immune Profile of the Nasal Mucosa in Patients with Cutaneous Leishmaniasis

María J. Gómez-Zafra, Adriana Navas, Jimena Jojoa, Julieth Murillo, Camila González, María Adelaida Gómez
De'Broski R. Herbert, Editor
María J. Gómez-Zafra
aCentro de Investigaciones en Microbiología y Parasitología Tropical (CIMPAT), Universidad de Los Andes, Bogotá, Colombia
bCentro Internacional de Entrenamiento e Investigaciones Médicas-CIDEIM, Cali, Colombia
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Adriana Navas
bCentro Internacional de Entrenamiento e Investigaciones Médicas-CIDEIM, Cali, Colombia
dUniversidad Icesi, Cali, Colombia
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Jimena Jojoa
bCentro Internacional de Entrenamiento e Investigaciones Médicas-CIDEIM, Cali, Colombia
dUniversidad Icesi, Cali, Colombia
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Julieth Murillo
bCentro Internacional de Entrenamiento e Investigaciones Médicas-CIDEIM, Cali, Colombia
cUniversidad Javeriana, Cali, Colombia
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Camila González
aCentro de Investigaciones en Microbiología y Parasitología Tropical (CIMPAT), Universidad de Los Andes, Bogotá, Colombia
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María Adelaida Gómez
bCentro Internacional de Entrenamiento e Investigaciones Médicas-CIDEIM, Cali, Colombia
dUniversidad Icesi, Cali, Colombia
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De'Broski R. Herbert
University of Pennsylvania
Roles: Editor
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DOI: 10.1128/IAI.00881-19
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    FIG 1

    Time after collection of NM samples affects cell recovery. Effect of sample processing time on (A) cell count and viability and (B) cell type frequency in NM samples (n = 4) processed immediately after sampling, at 0 h (light gray), and 8 h (gray) after sampling and storage at 4°C (B). Frequencies reported for CD66b+, CD14+ and CD3+ populations were calculated based on the total number of CD45+ cells in each sample. The Wilcoxon test was used to estimate statistical significance and P values.

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    FIG 2

    Cellular composition of the nasal mucosa of healthy volunteers and CL patients. Frequency of (A) EPCAM+ and CD45+ cells and (B) CD45+/CD3+, CD45+/CD14+, and CD45+/CD66b+ populations in NM curette samples from healthy volunteers (n = 9) and CL patients (n = 5). Data are shown as mean ± SD. The Kruskal-Wallis test was used to estimate statistical significance and P values.

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  • TABLE 1

    Demographic characteristics of study participantsa

    TABLE 1
    • ↵a CL, cutaneous leishmaniasis; NA, not applicable.

    • ↵b Strains were not isolated either because the culture was contaminated or because the patient did not consent for parasite isolation.

  • TABLE 2

    Differentially expressed genes in NM swab samples from CL patientsa

    TABLE 2
    • ↵a CL, cutaneous leishmaniasis; NM, nasal mucosa; SD, standard deviation; CLn+, patients with Leishmania-positive NM samples; CLn−, patients with Leishmania-negative NM samples. Boldface indicates significantly different gene expression.

    • ↵b Gene expression values (2−ΔCt) of differentially modulated genes in NM swab samples.

    • ↵c P values were derived from the Mann-Whitney test.

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      Table S1 to S3; Fig. S1 to S3

      PDF, 931K

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Immune Profile of the Nasal Mucosa in Patients with Cutaneous Leishmaniasis
María J. Gómez-Zafra, Adriana Navas, Jimena Jojoa, Julieth Murillo, Camila González, María Adelaida Gómez
Infection and Immunity Apr 2020, 88 (5) e00881-19; DOI: 10.1128/IAI.00881-19

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Immune Profile of the Nasal Mucosa in Patients with Cutaneous Leishmaniasis
María J. Gómez-Zafra, Adriana Navas, Jimena Jojoa, Julieth Murillo, Camila González, María Adelaida Gómez
Infection and Immunity Apr 2020, 88 (5) e00881-19; DOI: 10.1128/IAI.00881-19
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    • ABSTRACT
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KEYWORDS

persistence
Th1/Th2
asymptomatic infection
cutaneous leishmaniasis
nasal mucosa

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