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Molecular Pathogenesis

Salmonella enterica Serovars Dublin and Enteritidis Comparative Proteomics Reveals Differential Expression of Proteins Involved in Stress Resistance, Virulence, and Anaerobic Metabolism

A. Y. Martinez-Sanguiné, B. D’Alessandro, M. Langleib, G. M. Traglia, A. Mónaco, R. Durán, J. A. Chabalgoity, L. Betancor, L. Yim
Manuela Raffatellu, Editor
A. Y. Martinez-Sanguiné
aDepartamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
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B. D’Alessandro
aDepartamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
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M. Langleib
aDepartamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
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G. M. Traglia
aDepartamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
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A. Mónaco
aDepartamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
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R. Durán
bInstituto Pasteur de Montevideo, Montevideo, Uruguay
cInstituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay
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J. A. Chabalgoity
aDepartamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
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L. Betancor
dDepartamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
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L. Yim
aDepartamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
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Manuela Raffatellu
University of California San Diego School of Medicine
Roles: Editor
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DOI: 10.1128/IAI.00606-20
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ABSTRACT

The Enteritidis and Dublin serovars of Salmonella enterica are phylogenetically closely related yet differ significantly in host range and virulence. S. Enteritidis is a broad-host-range serovar that commonly causes self-limited gastroenteritis in humans, whereas S. Dublin is a cattle-adapted serovar that can infect humans, often resulting in invasive extraintestinal disease. The mechanism underlying the higher invasiveness of S. Dublin remains undetermined. In this work, we quantitatively compared the proteomes of clinical isolates of each serovar grown under gut-mimicking conditions. Compared to S. Enteritidis, the S. Dublin proteome was enriched in proteins linked to response to several stress conditions, such as those encountered during host infection, as well as to virulence. The S. Enteritidis proteome contained several proteins related to central anaerobic metabolism pathways that were undetected in S. Dublin. In contrast to what has been observed in other extraintestinal serovars, most of the coding genes for these pathways are not degraded in S. Dublin. Thus, we provide evidence that S. Dublin metabolic functions may be much more affected than previously reported based on genomic studies. Single and double null mutants in stress response proteins Dps, YciF, and YgaU demonstrate their relevance to S. Dublin invasiveness in a murine model of invasive salmonellosis. All in all, this work provides a basis for understanding interserovar differences in invasiveness and niche adaptation, underscoring the relevance of using proteomic approaches to complement genomic studies.

FOOTNOTES

    • Received 27 September 2020.
    • Returned for modification 28 October 2020.
    • Accepted 10 December 2020.
    • Accepted manuscript posted online 23 December 2020.
  • Supplemental material is available online only.

  • Copyright © 2021 American Society for Microbiology.

All Rights Reserved.

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Salmonella enterica Serovars Dublin and Enteritidis Comparative Proteomics Reveals Differential Expression of Proteins Involved in Stress Resistance, Virulence, and Anaerobic Metabolism
A. Y. Martinez-Sanguiné, B. D’Alessandro, M. Langleib, G. M. Traglia, A. Mónaco, R. Durán, J. A. Chabalgoity, L. Betancor, L. Yim
Infection and Immunity Feb 2021, 89 (3) e00606-20; DOI: 10.1128/IAI.00606-20

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Salmonella enterica Serovars Dublin and Enteritidis Comparative Proteomics Reveals Differential Expression of Proteins Involved in Stress Resistance, Virulence, and Anaerobic Metabolism
A. Y. Martinez-Sanguiné, B. D’Alessandro, M. Langleib, G. M. Traglia, A. Mónaco, R. Durán, J. A. Chabalgoity, L. Betancor, L. Yim
Infection and Immunity Feb 2021, 89 (3) e00606-20; DOI: 10.1128/IAI.00606-20
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KEYWORDS

comparative proteomics
Salmonella Dublin
Salmonella enteritidis
stress resistance
virulence
anaerobic metabolism

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