PT  - JOURNAL ARTICLE
AU  - Jouen-Beades, F
AU  - Paris, E
AU  - Dieulois, C
AU  - Lemeland, J F
AU  - Barre-Dezelus, V
AU  - Marret, S
AU  - Humbert, G
AU  - Leroy, J
AU  - Tron, F
TI  - In vivo and in vitro activation and expansion of gammadelta T cells during Listeria monocytogenes infection in humans.
DP  - 1997 Oct 01
TA  - Infection and Immunity
PG  - 4267--4272
VI  - 65
IP  - 10
4099  - http://iai.asm.org/content/65/10/4267.short
4100  - http://iai.asm.org/content/65/10/4267.full
SO  - Infect. Immun.1997 Oct 01; 65
AB  - Serial flow cytometry analyses of peripheral blood mononuclear cells obtained from 8 patients infected with Listeria monocytogenes showed a higher percentage (P < 0.01) of gammadelta T cells (median, 11.7; range, 3.7 to 35.3) than did 16 age-matched uninfected controls (1.7, 0.4 to 13). Most in vivo-expanded gammadelta T cells expressed the Vgamma9 and Vdelta2 gene products and displayed a memory phenotype (CD45RO[high]), and patients' gammadelta T cells expressed significantly more (P < 0.01) activation marker HLA-DR than did controls (19.8% [median] and 0.9 to 87.6% [range] versus 2.3% and 0 to 4.7%, respectively). When peripheral blood mononuclear cells from normal donors were cultured in vitro with heat-killed Listeria cells, analysis of CD25 and HLA-DR expression on gammadelta and alphabeta T cells indicated that a high percentage of gammadelta T cells was activated early compared to alphabeta T cells. In addition, depletion of gammadelta T cells before culture abrogated the early lymphocyte proliferative response induced by the pathogen. Taken together, these results argue for the involvement of gammadelta T cells during L. monocytogenes infection in humans.