TABLE 1.

Strains and plasmids used

Strain or plasmidDescriptionReference or source
Strains
    MZ100Wild typeMicrobia, Inc.
    ALC2713MZ100 agr::tetMThis study
    SMC2714MZ100 icaADBC::tetKThis study
    SMC2715MZ100 hla::ermCThis study
    ALC4007MZ100 sarA::aph3-AThis study
    ALC3741MZ100 sigB::ermCThis study
    8325-4Wild type58
    SMC10178325-4 hla::ermC46
    RN4220Wild type, DNA acceptor strain44
    Sa113Wild type, DNA acceptor strain29
    SMC1441Sa113 ica::tetK12
    SH1000Wild type27
    ALC3085SH1000 sigB::ermCThis study
Plasmids
    pALC2201Promoter of asp23 driving gfpuv56
    pALC1743RNAIII promoter regulating gfpuvr2
    pALC2111dsred replacing gfpuvr on pALC1743This study
    pSC23pBT5 with icaADBC::tetK12