TABLE 1.

Sequences of the primers used for quantitative real-time PCR

GeneDirectionaPrimer (5′ → 3′)Product size (bp)Final concn (nM)bReference
IFN-γForCCTGCGGCCTAGCTCTGA8110067a
RevCAGCCAGAAACAGCCATGAG100
IL-6ForACAACCACGGCCTTCCCTACTT12910031a
RevCACGATTTCACAACCACGGCCTTCCCTACTT100
MIP-2ForATCCAGAGCTTGAGTGTGACGC9010031a
RevAAGGCAAACTTTTTGACCGCC100
IL-12ForAGCAGTAGCAGTTCCCCTGA8820050a
RevAGTCCCTTTGGTCCAGTGTG200
MCP-1/JEForCCACTCACCTGCTGCTACTCAT7610031a
RevTGGTGATCCTCTTGTAGCTCTCC300
IL-10ForCTTGCACTACCAAAGCCACA8610050a
RevTAAGAGCAGGCAGCATAGCA100
TNF-αForGTGTGGGTGAGGAGCACGTA1981001
RevTCATCCTGCTCTTCTTTCTCGAAT100
GAPDHcForCATCGCCTTCCGTGTTCCTA54100http://medgen31.rug.ac .be/primerdatabase
RevGCGGCACGTCAGATCCA100
  • a For, forward; Rev, reverse.

  • b Final concentration of primer in 25 μl (final volume).

  • c GAPDH, glyceraldehyde-3-phosphate dehydrogenase.