TABLE 2.

Damage of reconstituted human epithelium by C. albicans strains

ExptType of strainsC. albicans strainRelevant genotypeAmt of LDH released (U/liter)a
A URA3 reconstitutedUninfected10 ± 0
CAF2-1Wild type460 ± 10
SPCa2 pmt1/pmt1 90 ± 0b
SPCa4 pmt2/PMT2 180 ± 10b
SPCa6 pmt4/pmt4 190 ± 10b
SPCa10 pmt5/pmt5 320 ± 10b
SPCa8 pmt6/pmt6 140 ± 10b
BPMT, reconstituted and controlsUninfected20 ± 0
CAI4Wild type250 ± 10
CAP1-3121(pRC18) pmt1/pmt1 150 ± 10
CAP1-3121(pCT30) pmt1/pmt1 (PMT1)210 ± 0b
CAP4-2164(pRC18) pmt4/pmt4 80 ± 0
CAP4-2164(pSP1) pmt4/pmt4 (PMT4)240 ± 10b
P5-5744(pRC18) pmt5/pmt5 270 ± 0
P5-5744(pBK1) pmt5/pmt5 (PMT5)270 ± 0
CAP2-2391(pRC18) pmt6/pmt6 110 ± 10
CAP2-2391(pCT34) pmt6/pmt6 (PMT6)270 ± 10b
  • a Mean ± standard deviation of three measurements.

  • b P < 0.0001 for a comparison of a pmt mutant and the wild-type strain in experiment A and for a comparison of a pmt mutant and the corresponding PMT-reconstituted strain in experiment B.