TABLE 1.

Strains and plasmids used in this study

Strain or plasmidRelevant genotype and phenotypeSource or reference
E. coli strains
    DH5αF− Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA116, 27
    DH5αλpirF− Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 λ::pir16, 27
    SM10λpirthi recA thr leu tonA lacY supE RP4-2-Tc::Mu λ::pirLaboratory strain
    AC-E1277SM10λpir (pAT1276), AprThis work
V. cholerae strains
    O395Classical biotype, Smr50
    AC-V61O395 lacZ::res-tet-res, spontaneous partial deletion of lacZ, Smr4
    AC-V1386AC-V61 ΔvieA, Smr51
    AC-V1596AC-V61 vieA(E170A), Smr51
    AC-V744O395 ΔtoxR, Smr26
    AC-V449O395 ΔtcpP, Smr57
    AC-V1296AC-V61 toxT-lacZ-tcpJ, SmrThis work
    AC-V1382AC-V1386 toxT-lacZ-tcpJ, SmrThis work
    AC-V1848AC-V1596 toxT-lacZ-tcpJ, SmrThis work
    AC-V1302AC-V1296 toxR::pGP704 Smr AprThis work
    AC-V1460AC-V61 pBAD33, Smr Cmr51
    AC-V1726AC-V61 pAT1662, Smr Cmr51
    AC-V1463AC-V1386 pBAD33, Smr Cmr51
    AC-V1464AC-V1386 pAT1337, Smr Cmr51
    AC-V1660AC-V1386 pAT1568, Smr CmrThis work
    AC-V1661AC-V1386 pAT1615, Smr CmrThis work
    AC-V1843AC-V1386 pAT1647, Smr CmrThis work
    AC-V1109O395 pMMB67EH, Smr AprThis work
    AC-V1815AC-V1596 pMMB67EH, Smr AprThis work
    AC-V1824AC-V1596 pAT1822, Smr AprThis work
    AC-V1869AC-V61 vpsR::pGP704 Smr AprThis work
    AC-V1870AC-V1596 vpsR::pGP704 Smr AprThis work
Plasmids
    pCVD442oriR6K mobRP4 sacB, Apr11
    pAT1276pCVD442::toxT-lacZ-tcpJ, AprThis work
    pGP704oriR6K mobRP4, Apr38
    pAC396pGP704::‘toxR', Apr38
    pAT1868pGP704::‘vpsR', AprThis work
    pCR-Scriptfl (+) ori, AprStratagene
    pGEM-Tfl ori, AprPromega
    pAT856pGEM-T::‘rpoB', Apr52
    pAT853pGEM-T::‘ctxA', Apr52
    pDSM701pGEM-T::‘toxT', Apr52
    pDSM702pGEM-T::‘ompT', Apr36
    pDSM703pGEM-T::‘ompU', Apr36
    pAT1809pGEM-T::‘tcpP', AprThis work
    pMMB67EHIncQ broad-host-range cloning vector, Apr40
    pAT1822pMMB67EH::vieA, AprThis work
    pBAD33pACYC184 ori, araC ParaBAD, Cmr15
    pAT1337pBAD33::vieA, Cmr48
    pAT1568pBAD33::VieA-EAL-His6, Cmr51
    pAT1615pBAD33::VieAEAL(E170A)-His6, Cmr51
    pAT1647pBAD33::‵RocS-His6, Cmr51
    pAT1662pBAD33::VCA0956-His6, Cmr51