TABLE 2.

Oligonucleotide primers used in this studya

Oligonucleotide nameSequence 5′ - 3′
toxTF1GCTCTAGATGTCAAACCATATCAGCCTAG
TZJR1AACTGCAGAGGATCAAGTAAACGTATTCC
5JF1GGGGTACCCTACAGTATTTAACTCAACCA
3JR1GCGAGCTCCTTCATTTTAGCTCACATTAA
TZJF1AACTGCAGTTTANGATACATTTTTATGACCATGATTACGGATTCA
CZBR1GGGGTACCTTATTTTTGACACCAGACCAA
tcpPF1TCAATTTCCCGATAACCTTT
tcpPR1AGTCAGCTTCATCAACAACG
F38GATGGAGATTTTGGCTTCA
vieARAACTGCAGTAGGTACAGCCATAACTCTCG
tcpEF2GGAGTTATCTATGACCCTGTT
lacZRGAGGGGACGACGACAGTATC
oriRCAGCAGTTCAACCTGTTG
toxRR2ATGGCATCGTTAGGGTTAG
vpsRF2TGGCATGCTATGCCTATGAAGCGTTTG
vpsRR2GCGAGCTCGTAGCACGATATCCGATGC
vpsRF3CATGCTGAAGTTGGAAAA
  • a Restriction enzyme sites are indicated by underlining.