TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant feature(s)Source or reference
Strains
    Y. enterocolitica
        JB580vSerogroup O:8, Nalr ΔyenR (R M+)21
        GY1087yscR::mTn5-Km2 ysaV::pEP185.239
        GY4428ysaV::pEP185.2This study
        GY4478pYV808139
        GY4531crp::str32
        GY4572ysaU::TnMod-lacZYA-RKm′This study
        GY4573orf6 orf7::TnMod-lacZYA-RKm′This study
        GY4574ddhB::TnMod-lacZYA-RKm′This study
        GY4613rcsB::TnMod-lacZYA-RKm′This study
        GY4639ysaR::TnMod-lacZYA-RKm′This study
        GY4641ysaE::TnMod-lacZYA-RKm′This study
        GY4664ysaU::TnMod-lacZYA-RKm′This study
        GY4665ysaC::TnMod-lacZYA-RKm′This study
        GY4668orf6 orf7::TnMod-lacZYA-RKm′This study
        GY4675ysaU::TnMod-lacZYA-RKm′ ysrS::pEP185.2This study
        GY4676ysaU::TnMod-lacZYA-RKm′ rcsB::pEP185.2This study
        GY4677ysaR::TnMod-lacZYA-RKm′ ysrS::pEP185.2This study
        GY4678ysaR::TnMod-lacZYA-RKm′ rcsB::pEP185.2This study
        GY4684pYV8081 ysaV::pEP185.2This study
        GY4737rcsB::pEP185.2This study
        GY4742ysrS::mTn5-Km2-lacZYAThis study
        GY4887ysrS::pEP185.2This study
        GY4888ysaE::TnMod-lacZYA-RKm′ rcsB::pEP185.2This study
        GY4891orf6 orf7::TnMod-lacZYA-RKm′ rcsB::pEP185.2This study
        GY4892ysaE::TnMod-lacZYA-RKm′ ysrS::pEP185.2This study
        GY4893rcsB::TnMod-lacZYA-RKm′ ysrS::pEP185.2This study
        GY4894ysrS::pFUSE (ysrS-lacZYA)This study
        GY4899orf6 orf7::TnMod-lacZYA-RKm′ ysrS::pEP185.2This study
        GY5019sycB::mTn5-Km2-lacZYAThis study
        GY5022sycB::mTn5-Km2-lacZYA ysrS::pEP185.2This study
        GY5021sycB::mTn5-Km2-lacZYA rcsB::pEP185.2This study
        GY5124rcsB::aacC1 GmrThis study
        GY5196rcsB::aacC1 ysrS::pFUSE (ysrS-lacZYA)This study
        YVM356yscR::mTn5-Km29
    E. coli S17-1 λpirrecA thi pro hsdR hsdM+ RP4::2-Tc::Mu::Km Tn7 λpir27
Plasmids
    p34s-GmGmr plasmid with antibiotic resistance cassette, accC111
    pCR-Blunt II-TOPOKmrInvitrogen
    pEP185.2Cmr, suicide vector21
    pFUSECmr, suicide vector for transcriptional fusions to lacZYA1
    pTM100mob+ oriR6K Tetr Cmr24
    pTnMod-lacZYA-RKm′TnMod-lacZYA-RKm′ in a delivery vector, KmrAndrew Darwin, NYU
    pREV10mTn5-Km2-lacZYA′ in a delivery vector, KmrVirginia Miller, Washington University
    pGY100Pcat-yplAB derivative of pTM10039
    pGY348Ca. 0.9-kb fragment of ysaV in pCR-Blunt II-TOPOThis study
    pGY352Ca. 0.9-kb fragment of ysaV in pEP185.2This study
    pGY431Ca. 0.5-kb fragment of rcsB in pCR-Blunt II-TOPOThis study
    pGY435Ca. 0.5-kb fragment of rcsB in pEP185.2This study
    pGY454ysrRS in pCR-Blunt II-TOPOThis study
    pGY482ysrRS in pTM100This study
    pGY512Ca. 0.5-kb fragment of ysrS in pCR-Blunt II-TOPOThis study
    pGY526Ca. 0.5-kb fragment of ysrS in pEP185.2This study
    pGY529Ca. 1-kb fragment of ysrS in pCR-Blunt II-TOPOThis study
    pGY530Ca. 1-kb fragment of ysrS in pFUSEThis study
    pGY551rcsB in pCR-Blunt II-TOPOThis study
    pGY552ysrRS in pTM100 with cat removedThis study
    pGY553rcsB in pTM100This study
    pGY556pTM100 with cat removedThis study
    pGY557rcsB::accC1 in pCR-Blunt II-TOPOThis study
    pGY561rcsB::accC1 in pEP185.2This study