TABLE 2.

Primers

PrimerSequenceaWild-type amplicon size (kb)
fliA-UPPER5′-TAG CTG AAT CGG TGA GTA AT-3′
fliA-LOWER5′-TCC TAT TAA AGT CAA AGT ATC CAT-3′0.9
fliAUP2-EcoR15′-ACG GGA ATT CAT AAC TAC AAT CTG CTG TTA TTG-3′
fliADOWN-BamH15′-ACG GGA TCC TTA TTA TTC CGG TAA TCT TGA TCT A-3′0.75
dotAUpper2165L5′-CGC ATT GGT ACT AGC CTT TCG TTA-3′
dotALower2166L5′-ACC ATC CTC ATA TTC CAC TTC CTT-3′3.4
fliD_UP5′-TGG CGC AAT AGG TGG AAC TG-3′
fliD_DOWN5′-TGG CGG CAA ATA ATG GAA AAA A-3′3.4
flhB_UP5′-TGG CCC ATG GTG CGT ATT-3′
flhB_DOWN5′-AGG GGA ACT TAT GGC CAA CTT ATC-3′3.4
flhB-Int_Down-EcoRV5′-ACG GAT ATC CCA AGT CCG GTA AAT AAT AAA ATG-3′
FlhB-Int-UP5′-ACG ATG ATG AGC AAT CAC TAG TGG GGT AAT-3′NAb
flaAUP5′-CTC CAG CCC AGC AGC CAC TTC-3′
flaADOWN5′-GAG CAC CCA TAT TAG CCC GAT TAG-3′1.9
flaA-Sacl5′-CGA GCT CTC TCA AGC GTG CGG AAA TG-3′
flaA-Kpn15′-CGG GGT ACC GCC CTG GCT ATC TTG AAC TAT GC-3′3.0
motA85105′-CAA GAT TAC CGG AAT AAA AAA TAA G-3′
motB85115′-TTT AAT AAA TAA TTA CCC AAG AG-3′1.6
BamHI-MotA55′-GCG CGG ATC CGC GAG CAG GAT TTG AAT TTG AAG G-3′
SalI-MotB35′-GCA CGT CGA CGC CTT TTA ATA AAT AAT TAC CCA AG-3′1.7
fliI_UP5′-TTA ATT CAA AAG CCC GTT CA-3′
fliI_DOWN3′-CCA CCT CCC CTT TAT ATT GTT TAG-3′2.3
  • a Underlined nucleotides indicate restriction sites.

  • b NA, not applicable.