TABLE 3.

Association (kon) and dissociation (koff) rate constants and equilibrium dissociation constants (KD) for BoNT/A IgG binding to BoNT/A1 and BoNT/A2a

AntibodyBoNT/A1BoNT/A2
KD (M−1)kon (M−1 s−1)koff (s−1)KD (M−1)kon (M−1 s−1)koff (s−1)
C252.98 × 10−101.5 × 1064.47 × 10−41.65 × 10−72.09 × 1043.63 × 10−3
S251.69 × 10−94.82 × 1058.15 × 10−42.14 × 10−61.34 × 1032.87 × 10−3
3D121.68 × 10−101.45 × 1062.44 × 10−41.04 × 10−93.48 × 1053.62 × 10−4
B41.8 × 10−97.2 × 1051.31 × 10−3NMNMNM
7C15.9 × 10−92.89 × 1051.71 × 10−35.1 × 10−93.38 × 1051.73 × 10−3
9D81.21 × 10−91.73 × 1052.11 × 10−41.3 × 10−92.08 × 1052.73 × 10−4
  • a Association and dissociation rate constants were determined by surface plasmon resonance in a BIAcore, and KD was calculated as koff/kon. NM, not measurable.