TABLE 3.

O-antigen genes and gene products of F. novicida

Gene or gene productLength (aa)aFunctionSimilar protein(s)% Identity with F. tularensis SchuS4c% PositivedOrganismAccession no.
IstuF2181bTransposaseISFtu295 (173/181)96 (175/181)F. tularensisYP_170453
wbtAFn368dTDP-glucose 4,6-dehydrataseWbtA98 (99/101)99 (100/101)F. novicidaYP_170401
wbtN371AminotransferaseUnnamed64 (235/366)80 (293/366)Bacillus cereusNP_981686
DegT, DnrJ, EryC1, StrS63 (231/365)78 (287/365)Clostridium thermocellum ATCC 27405ZP_00510645
wbtO209UDP-galactose phosphate transferaseUnnamed62 (128/204)80 (165/204)Oceanobacillus iheyensisNP_693814
PglC62 (126/201)76 (154/201)C. jejuniZP_01099808
wbtP220AcetyltransferaseUnnamed41 (84/201)61 (123/201)Oceanobacillus iheyensisNP_693813
PglB38 (76/199)55 (111/199)N. meningitidisAAC25979
wbtDFn363Galacturonosyl transferaseWbtD86 (310/359)93 (334/359)F. novicidaYP_170398
wbtEFn401UDP-glucose/GDP-mannose dehydrogenaseWbtE98 (393/401)98 (396/401)F. novicidaYP_170397
wbtFFn328NAD-dependent epimerase; UDP-glucose 4 epimeraseWbtF98 (315/320)99 (318/320)F. novicidaYP_170396
wzy430Capsule biosynthesisCapE22 (94/427)40 (172/427)Staphylococcus aureusAAA64644
O-Antigen polymeraseWzy20 (64/314)44 (140/314)E. coliAAT77177
wbtGFn371Glycosyl transferaseWbtG89 (324/361)95 (345/361)F. novicidaYP_170394
wbtQ378Glycosyl transferaseWbnK26 (92/345)47 (163/345)S. dysenteriaeAAO39702
WbqHEscherichia coliAAR97066
wbtHFn630Asparagine synthase; glutamine amidotransferaseWbtH97 (611/628)98 (618/628)F. novicidaYP_170393
wzx415O-Antigen flippaseWzx25 (102/398)54 (217/398)E. coliAAK60454
Transposase156TransposaseTransposase55 (79/142)72 (103/142)N. meningitidisAAP44503
  • a aa, amino acids.

  • b Partial sequence data.

  • c Values in parentheses indicate number of invariant residues/total number of residues.

  • d Values in parentheses indicate number of invariant and/or conserved residues/total number of residues.