TABLE 3.

O-antigen genes and gene products of F. novicida

Gene or gene productLength (aa)aFunctionSimilar protein(s)% Identity with F. tularensis SchuS4c% PositivedOrganismAccession no.
IstuF2181bTransposaseISFtu295 (173/181)96 (175/181) F. tularensis YP_170453
wbtA Fn 368dTDP-glucose 4,6-dehydrataseWbtA98 (99/101)99 (100/101) F. novicida YP_170401
wbtN 371AminotransferaseUnnamed64 (235/366)80 (293/366) Bacillus cereus NP_981686
DegT, DnrJ, EryC1, StrS63 (231/365)78 (287/365) Clostridium thermocellum ATCC 27405 ZP_00510645
wbtO 209UDP-galactose phosphate transferaseUnnamed62 (128/204)80 (165/204) Oceanobacillus iheyensis NP_693814
PglC62 (126/201)76 (154/201) C. jejuni ZP_01099808
wbtP 220AcetyltransferaseUnnamed41 (84/201)61 (123/201) Oceanobacillus iheyensis NP_693813
PglB38 (76/199)55 (111/199) N. meningitidis AAC25979
wbtD Fn 363Galacturonosyl transferaseWbtD86 (310/359)93 (334/359) F. novicida YP_170398
wbtE Fn 401UDP-glucose/GDP-mannose dehydrogenaseWbtE98 (393/401)98 (396/401) F. novicida YP_170397
wbtF Fn 328NAD-dependent epimerase; UDP-glucose 4 epimeraseWbtF98 (315/320)99 (318/320) F. novicida YP_170396
wzy 430Capsule biosynthesisCapE22 (94/427)40 (172/427) Staphylococcus aureus AAA64644
O-Antigen polymeraseWzy20 (64/314)44 (140/314) E. coli AAT77177
wbtG Fn 371Glycosyl transferaseWbtG89 (324/361)95 (345/361) F. novicida YP_170394
wbtQ 378Glycosyl transferaseWbnK26 (92/345)47 (163/345) S. dysenteriae AAO39702
WbqH Escherichia coli AAR97066
wbtH Fn 630Asparagine synthase; glutamine amidotransferaseWbtH97 (611/628)98 (618/628) F. novicida YP_170393
wzx 415O-Antigen flippaseWzx25 (102/398)54 (217/398) E. coli AAK60454
Transposase156TransposaseTransposase55 (79/142)72 (103/142) N. meningitidis AAP44503
  • a aa, amino acids.

  • b Partial sequence data.

  • c Values in parentheses indicate number of invariant residues/total number of residues.

  • d Values in parentheses indicate number of invariant and/or conserved residues/total number of residues.