TABLE 4.

Genes identified as having lower expression in E. dispar SAW760 and E. histolytica Rahman than in E. histolytica HM-1:IMSSa

TIGR locusbTIGR gene productMicroarray hybridization ratioGene size (nt)% Nucleotide identityc% of locus found in databased
E. dispar/ E. histolytica HM-1:IMSSE. histolytica Rahman/ E. histolytica HM-1:IMSS
32.m00218Serine palmitoyltransferase0.370.392,6499462
2.m00567Conserved hypothetical protein0.620.409129185
343.m0007426S proteasome regulatory subunit0.520.491,0719468
269.m00084Mitochondrial carrier protein0.250.448319577
143.m00082Protein kinase, putative0.230.351,68696100
312.m00036Hypothetical protein0.070.087,8668935
238.m00054Hypothetical protein0.100.097,8158935
196.m00089Conserved hypothetical protein0.310.478638956
296.m00047Hypothetical protein0.170.311,0928799
7.m00396Hypothetical protein0.040.13621No hitsNo hits
209.m00109Hypothetical protein0.300.312888156
225.m00057Hypothetical protein0.180.16828No hitsNo hits
394.m00058Hypothetical protein0.080.158438219
11.m00354Hypothetical protein0.240.281,242917
120.m00092Hypothetical protein0.360.296308825
112.m00118Acyl-coenzyme A synthetase, putative0.270.4419479548
612.m00020Hypothetical protein0.100.236788926
29.m00210hsp70 family protein0.550.451,5708861
209.m00108Hypothetical protein0.500.32243No hitsNo hits
864.m00008Conserved hypothetical protein0.550.301,1728739
80.m00165Hypothetical protein0.100.302,9888916
194.m00115Protein kinase, putative0.410.342,7279523
27.m00257Reverse transcriptase, putative0.210.161,0118985
393.m00037Hypothetical protein0.300.36786No hitsNo hits
147.m00110Conserved hypothetical protein0.370.271,1528942
543.m00021Hypothetical protein0.440.443219085
244.m00078Hypothetical protein0.060.12612No hitsNo hits
711.m00021Hypothetical protein0.240.333428456
460.m00024Conserved hypothetical protein0.100.326128578
  • a The first five loci listed are highly conserved in E. dispar SAW760 (≥90% nucleotide identity over ≥50% of the locus).

  • b Genes confirmed by Northern blot analysis are shown in bold.

  • c Compared to E. dispar SAW760 ortholog.

  • d E. dispar SAW760 database.