TABLE 3.

Summary of the effects of IDO and iNOS activities on L2 growtha

Cell linebProduction or growth with additive(s)c
NoneIFN-γLPSIFN-γ-LPSIFN-γ-TNF-α-IL-1β-LPS
IDOiNOSIFUIDOiNOSIFUIDOiNOSIFUIDOiNOSIFUIDOiNOSIFU
HeLa (HE)d8.30 ± 0.76915.14 ± 0.338.28 ± 0.74875.16 ± 0.31885.22 ± 0.46
HL (HE)7.53 ± 0.60895.35 ± 0.357.80 ± 0.63935.10 ± 0.40NDeNDND
A549 (HE)7.71 ± 0.65945.39 ± 0.407.69 ± 0.71885.33 ± 0.29875.41 ± 0.32
RT-4 (HE)7.53 ± 0.71925.59 ± 0.367.50 ± 0.60895.21 ± 0.334975.19 ± 0.29
T24 (HE)7.96 ± 0.72925.56 ± 0.607.80 ± 0.71945.60 ± 0.36915.67 ± 0.45
BeWo (HE)7.49 ± 0.637.46 ± 0.547.51 ± 0.597.47 ± 0.55NDNDND
IEC4.1 (ME)6.96 ± 0.595.16 ± 0.436.79 ± 0.555.32 ± 0.38NDNDND
TM3 (ME)6.83 ± 0.555.16 ± 0.346.82 ± 0.505.26 ± 0.42NDNDND
MLE-15 (ME)8.19 ± 0.73397.90 ± 0.718.20 ± 0.62417.80 ± 0.59NDNDND
BM12.4 (ME)48.37 ± 0.68176.72 ± 0.7138.24 ± 0.71226.45 ± 0.61NDNDND
BM1.11 (ME)127.17 ± 0.64255.37 ± 0.46147.24 ± 0.65295.46 ± 0.33NDNDND
McCoy (MF)38.20 ± 0.81216.18 ± 0.5068.19 ± 0.70256.15 ± 0.42NDNDND
L929 (MF)28.11 ± 0.77116.31 ± 0.5327.99 ± 0.63136.27 ± 0.57NDNDND
THP-1 (HM)7.96 ± 0.62945.66 ± 0.397.89 ± 0.52935.36 ± 0.51NDNDND
RAW 264 (MM)126.19 ± 0.73884.53 ± 0.47824.34 ± 0.33904.25 ± 0.34NDNDND
RAW NO (MM)16.29 ± 0.71394.76 ± 0.52116.19 ± 0.47474.62 ± 0.29NDNDND
J774 (MM)86.23 ± 0.66505.17 ± 0.49316.15 ± 0.59425.20 ± 0.38NDNDND
  • a Growth was assessed by determining the number of infectious EBs recovered 48 h Postinfection. Growth data are presented as IFU (log10) and represent the means ± standard errors SE of triplicate determinations. Tryptophan and nitrite data presented are the averages of two determinations. For tryptophan the averaged 100% control values in pmol/107 cells are as follows: HeLa, 7,247; HL, 7,432; A549, 7,789; RT-4, 6,459; T24, 6,483; BeWo, 7,256; MLE15, 5,019.

  • b HE, human epithelial line; ME, murine epithelial; MF, murine fibroblast; HM, human monocyte; MM, murine macrophage.

  • c Cell culture additives: IFN-γ, 20 U/ml; LPS, 20 ng/ml; TNF-α, 20 ng/ml; IL-1β, 20 ng/ml.

  • d —, no evidence of IDO or iNOS production as judged by RT-PCR, western blotting, and/or bioassay.

  • e ND, not determined.