TABLE 2.

Summary of IDO and iNOS activities in human and mouse cells

Cell linebProduction with additive(s)c
NoneIFN-γLPSIFN-γ-LPSIFN-γ-TNF-α-IL-1β-LPS
IDOiNOSIDOiNOSIDOiNOSIDOiNOSIDOiNOS
HeLa (HE)a949692
HL (HE)9592NDdND
A549 (HE)868891
RT-4 (HE)93963627
T24 (HE)919087
BeWo (HE)ND
IEC4.1 (ME)NDND
TM3 (ME)NDND
MLE-15 (ME)3942NDND
BM12.4 (ME)NDND
BM1.11 (ME)NDND
McCoy (MF)44351NDND
L929 (MF)19424NDND
THP-1 (HM)1190NDND
RAW 264.7 (MM)3270118NDND
RAW 264 NO (MM)659NDND
J774 (MM)63282NDND
  • a —, no evidence of IDO or iNOS production as judged by RT-PCR, Western blotting, and/or bioassay. When IDO was detected results are given as percent reduction in tryptophan pool compared to untreated control, and when iNOS was detected results are given in μM nitrite produced. Results presented are the averages of two determinations. For tryptophan the averaged 100% control values in pmole/107 cells are as follows: HeLa, 7,653; HL, 7,450; A549, 8,054; RT-4, 6,371; T24, 6,543; BeWo, 7,606; MLE15, 5,276.

  • b HE, human epithelial line; ME, murine epithelial line; MF, murine fibroblast; HM, human macrophage; MM, murine macrophage.

  • c Cell culture additives: IFN-γ, 20 U/ml; LPS, 20 ng/ml; TNF-α, 20 ng/ml; IL-1β, 20 ng/ml.

  • d ND, not determined.