TABLE 1.

Primers used for site-directed mutagenesis

PrimeraSequenceAmino acid change
Δ1 fwd5′-CGATTTGTTGGCTAAGCAAGATTATGGTCAGG-3′ΔW134H135
Δ1 rev5′-CCTGACCATAATCTTGCTTAGCCAACAAATCG-3′
Δ2 fwd5′-GTTGGCTAAGTGGCATTATGGTCAGGTCAATAATGTCCC-3′ΔQ136D137
Δ2 rev5′-GGGACATTATTGACCTGACCATAATGCCACTTAGCCAAC-3′
Δ3 fwd5′-GGCTAAGTGGCATCAAGATCAGGTCAATAATGTCCC-3′ΔY138G139
Δ3 rev5′-GGGACATTATTGACCTGATCTTGATGCCACTTAGCC-3′
Δ4 fwd5′-GGCATCAAGATTATGGTAATAATGTCCCAGCTAG-3′ΔQ140V141
Δ4 rev5′-CTAGCTGGGACATTATTACCATAATCTTGATGCC-3′
Δ5 fwd5′-GGTCAGGTCAATAATGCTAGAATGCAGTATG-3′ΔV144P145
Δ5 rev5′-CATACTGCATTCTAGCATTATTGACCTGACC-3′
Δ6 fwd5′-GGTCAATAATGTCCCAATGCAGTATGAAAAAATAACGGCTC-3′ΔA146R147
Δ6 rev5′-GAGCCGTTATTTTTTCATACTGCATTGGGACATTATTGACC-3′
Δ7 fwd5′-GGTCAATAATGTCCCAGCTAGATATGAAAAAATAACGGCTC-3′ΔM148Q149
Δ7 rev5′-GAGCCGTTATTTTTTCATATCTAGCTGGGACATTATTGACC-3′
Δ8 fwd5′-GTCCCAGCTAGAATGCAGAAAATAACGGCTCACAGC-3′ΔY150E151
Δ8 rev5′-GCTGTGAGCCGTTATTTTCTGCATTCTAGCTGGGAC-3′
ΔA146 fwd5′-GGTCAGGTCAATAATGTCCCAAGAATGCAGTATGAAAAAATAAC-3′ΔA146
ΔA146 rev5′-GAGCCGTTATCATACTGCATTCTTGGGACATTATTGACCTGACC-3′
  • a fwd, forward; rev, reverse.