TABLE 4.

Summary of the effects of IDO and iNOS activities on MoPn growtha

Cell linebProduction or growth with additive(s)c
NoneIFN-γLPSIFN-γ-LPS
IDOiNOSIFUIDOiNOSIFUIDOiNOSIFUIDOiNOSIFU
HeLa (HE)d8.26 ± 0.57905.26 ± 0.418.22 ± 0.69925.18 ± 0.43
HL (HE)7.93 ± 0.63935.79 ± 0.338.11 ± 0.81945.69 ± 0.54
A549 (HE)8.12 ± 0.78915.57 ± 0.377.84 ± 0.75915.91 ± 0.45
RT-4 (HE)7.79 ± 0.82885.80 ± 0.427.72 ± 0.82935.51 ± 0.44
T24 (HE)7.88 ± 0.49936.10 ± 0.597.99 ± 0.67896.15 ± 0.61
BeWo (HE)8.12 ± 0.668.11 ± 0.688.1 ± 0.698.11 ± 0.74
IEC4.1 (HE)7.11 ± 0.536.53 ± 0.717.11 ± 0.726.60 ± 0.61
TM3 (ME)6.93 ± 0.386.53 ± 0.626.79 ± 0.716.33 ± 0.54
MLE-15 (ME)8.39 ± 0.87448.40 ± 0.778.41 ± 0.73428.41 ± 0.41
BM12.4 (ME)28.40 ± 0.5478.39 ± 0.8128.41 ± 0.68108.38 ± 0.67
BM1.11 (ME)58.12 ± 0.61108.11 ± 0.8368.12 ± 0.77127.85 ± 0.72
McCoy (MF)27.70 ± 0.71156.74 ± 0.5327.73 ± 0.56206.70 ± 0.59
L929 (MF)38.10 ± 0.7667.21 ± 0.667.89 ± 0.627.27.17 ± 0.68
THP-1 (HM)7.53 ± 0.48935.59 ± 0.597.32 ± 0.69945.31 ± 0.39
RAW 264 (MM)166.44 ± 0.61414.88 ± 0.51325.27 ± 0.42565.11 ± 0.46
RAW NO (MM)7.20 ± 0.77216.46 ± 0.5367.12 ± 0.58596.29 ± 0.51
J774 (MM)76.67 ± 0.69525.36 ± 0.37296.67 ± 0.71545.58 ± 0.44
  • a Growth was assessed by determining the number of infectious EBs recovered 48 h postinfection. Growth data are presented as IFU (log10) and represent the means ± standard errors of triplicate determinations. Tryptophan and nitrite data presented are the averages of two determinations. For tryptophan the averaged 100% control values in pmol/107 cells are as follows: HeLa, 7,734; HL, 7,653; A549, 7,813; RT-4, 6,216; T24, 6,167; BeWo, 7,237; MLE15, 5,106.

  • b HE, human epithelial line; ME, murine epithelial; MF, murine fibroblast; HM, human monocyte; MM, murine macrophage.

  • c Cell culture additives: IFN-γ, 20 U/ml; LPS, 20 ng/ml.

  • d —, no evidence of IDO or iNOS production.