TABLE 1.

Primers used for amplification of groEL genes from different bacteria

BacteriaGene productRestriction sitePrimer sequencea
L. johnsonii NCC 533La1 NcoI5′-CATATACCATGGCTAAAGAGATTAAATTTTCTGAAAATGCAAGACACTC
La1 SalI3′-GTTTCTGTCGACCATTCCCATACCCATACCTGG
L. helveticus ATCC 15009GroEL NcoI5′-ATATATCCATGGCAAAAGATATTAAATTCTCAGAAAACGC
GroEL BamHI3′-ATATATGGATCCATTCCCATTGGAGCACCTGC
B. subtilis NCC 199GroEL NcoI5′-ATATATCCATGGCAAAAGAAATTAAGTTTAGTGAAGAAGC
GroEL BamHI3′-ATATATGGATCCATCATTCCACCCATACC
L. lactis MG 1363GroEL NcoI5′-ATATATCCATGGCAAAAGAAATTAAATTTTCAAGTGATGC
GroEL BamHI3′-ATATATGGATCCATCATACCACCCATTCCC
H. pylori P1GroEL BamHI5′-CCGGGATCCCGGATGGCAAAAGAAATCAAATTTTCAG
GroEL SalI3′-GAATTCGTCGACTTACATCATGCCACCCATGCCTC
  • a Restriction sites are given in boldface.