TABLE 3.

Subset of genes with lower expression in E. dispar SAW760 than in E. histolytica HM-1:IMSS and with loci with significant orthologs in the E. dispar SAW760 databasea

TIGR locusbTIGR gene productMicroarray hybridization ratioGene size (nt)% Nucleotide identityc% of locus found in databased
E. dispar/ E. histolytica HM-1:IMSSE. histolytica Rahman/ E. histolytica HM-1:IMSS
20.m00330CXXC-rich protein0.250.823,3799276
6.m00454Lysozyme, putative0.470.926399699
9.m00419Fe hydrogenase, putative0.391.421,4079165
18.m00300Protein kinase, putative0.452.421,1889499
171.m00098Ankyrin repeat protein, putative0.581.3482893100
836.m00014ARP2/3 complex 21 kDa subunit, putative0.460.7958693100
110.m00129Rho family GTPase0.560.676399696
32.m00230BspA-like leucine rich repeat protein, putative0.541.161,2789399
344.m00046Ser/Thr protein phosphatase, putative0.250.631,55193100
283.m00063DEAD/DEAH box helicase, putative0.371.201,26094100
94.m00134Glycogen synthase, putative0.340.964,1289676
50.m00199Sec61 protein, putative0.430.812469686
30.m00257Rab family GTPase0.310.5764892101
1.m00628Protein disulfide isomerase, putative0.321.339909291
251.m00088Sir2 family transcriptional regulator, putative0.480.631,0799368
297.m00063CCAAT-box-binding transcription factor, putative0.401.072,15794100
87.m00163Potassium transporter, putative0.351.172,2029465
76.m00156Rab family GTPase0.500.987609568
176.m00112Pseudogene, peroxiredoxin0.420.686969491
8.m00352Phospholipid-transporting P-type ATPase, putative0.280.624,1249366
95.m00149Protein phosophatase 2C, putative0.321.352,9259166
143.m00082Protein kinase, putative0.530.931,68696100
16.m00300Gal/GalNAc lectin heavy subunit0.240.973,8619054
103.m00174Conserved hypothetical protein0.241.262,5869157
67.m00102Phosphatidylinositol 3-kinase, putative0.450.733,3309563
95.m00133Rab GTPase activating protein, putative0.480.921,9549460
29.m00231Sphingomyelinase C, putative0.170.5197595100
6.m00467Type A flavoprotein0.230.871,2219299
15.m00331Peptidyl-prolyl cis-trans isomerase, putative0.410.781,1859369
  • a Based on ≥90% nucleotide identity and/or ≥50% of the locus.

  • b Genes confirmed by Northern blot analysis are shown in bold.

  • c Compared to E. dispar SAW760 ortholog.

  • d E. dispar SAW760 database.