TABLE 2.

Subset of genes with lower expression in E. dispar SAW760 than in E. histolytica HM-1:IMSS and with loci not highly conserved in the E. dispar SAW760 databasea

TIGR locusbTIGR gene productMicroarray hybridization ratioGene size (nt)% Nucleotide identityc% of locus found in databased
E. dispar/ E. histolytica HM-1:IMSSE. histolytica Rahman/ E. histolytica HM-1:IMSS
320.m00035Pseudogene, Gal/GalNAc lectin heavy subunit0.221.163,8708854
151.m00094Multidrug resistance protein, putative0.381.184,3989739
242.m00078Cysteine protease 10.311.279488560
79.m00156Cysteine protease 10.461.181,0058557
191.m00117Cysteine proteinase, putative0.091.079578265
13.m00317Sucrose transporter, putative0.151.101,386No hitsNo hits
116.m00130Ras GTPase activating protein, putative0.350.733,5519612
52.m00161Immunodominant variable surface antigen0.221.013,3429026
11.m00326tRNA intron endonuclease, putative0.540.774928628
5.m00482Protein kinase, putative0.314.523,5948834
114.m00128Rab family GTPase0.210.93667956
310.m00070BspA-like leucine-rich repeat protein, putative0.230.801,1978967
129.m00157DNA primase large subunit, putative0.140.731,3749047
501.m00019Cysteine protease 80.101.149488257
39.m00237Cortexillin, putative0.140.762,3769318
442.m00024Receptor protein kinase, putative0.361.706,6179628
151.m00093DNA repair protein, putative0.411.071,9828819
2.m00588Protein kinase, putative0.510.941,227926
126.m00100Rho GTPase activating protein, putative0.100.751,2429346
565.m00023AIG1 family protein, putative0.070.571,0958647
7.m00480Sec6 protein, putative0.390.652,3019528
53.m00209Heat shock protein 70, putative0.440.821,8988862
16.m00343Ubiquitin carboxyl-terminal hydrolase, putative0.310.872,42189100
136.m0010570-kDa heat shock protein, putative0.370.831,7278526
77.m00174Hypothetical protein (KERP1)0.220.68552No hitsNo hits
338.m00048Leucyl-tRNA synthetase, putative0.480.713,2199632
130.m00115Importin beta subunit, putative0.251.273,1159025
92.m00150Heat shock protein 90, putative0.170.582,1579837
77.m00153Glycogen debranching enzyme, putative0.321.134,3329419
178.m00100Sec24 protein, putative0.350.592,2839347
14.m00281Pseudogene, beta-adaptin0.240.902,5929830
57.m00152CCR4/NOT complex transcription factor subunit 40.580.672,1129649
60.m00136Conserved hypothetical protein0.551.252,5978621
296.m00051Rho family GTPase0.350.867469741
1.m00597SEC-24 protein, putative0.510.951,9449419
309.m00046Phospholipid-transporting P-type ATPase, putative0.431.663,2979438
160.m00087Surface antigen ariel1 related0.281.31648No hitsNo hits
  • a Based on <90% nucleotide identity and/or <50% of the locus.

  • b Genes confirmed by Northern blot analysis are shown in bold.

  • c Compared to E. dispar SAW760 ortholog.

  • d E. dispar SAW760 database.