Bacterial strains and plasmids used in this study

Strain or plasmidCharacteristicsaSource or reference
E. coli strains
    ER2566F λfhuA2 [lon] ompT lacZ::T7 gene1 gal sulA11 Δ(mcrC-mrr)114::IS10 R(mcr-73::miniTn10-TetS)2 R(zgb-210::Tn10)(TetS) endA1 [dcm]New England Biolabs, Inc.
    DH5αF φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK mK+) phoA supE44 λthi-1 gryA96 relA1Laboratory Collection
    pGEM7ZfCloning vector; AprPromega
    pTYB12N-terminal fusion expression vector in which the N terminus of a target protein is a fused intein tag; AprNew England Biolabs, Inc.
    pCMM2552.2-kb EcoRI fragment containing flaB and part of flaA cloned into pGEM7ZfThis study
    pCMM2501.5-kb EcoRI-PstI fragment containing ORF of flaB cloned into pTYB12This study
  • a Apr, ampicillin resistance.