TABLE 1.

TX-114-extracted proteins of L. interrogans serovar Copenhageni identified by mass spectrometry from Fig. 1a

Spot no.TIGR locusTIGR annotationMasspIScoreNo. of peptides
1NT03LI1637LipL3229612.886.807220217
2NT03LI2344Putative outer membrane protein17836.215.977416410
3NT03LI0012LipL2119660.977.798919413
4NT03LI0591Putative lipoprotein23476.715.22881397
5NT03LI1973LipL4542101.007.593143128
51215
6NT03LI0010Putative lipoprotein27700.766.732742624
2368
7NT03LI1637LipL3229612.886.80721138
311
8NT03LI1763LipL3127620.737.345456443
2349
9NT03LI1168OmpL133457.008.87261483
10NT03LI1168OmpL133457.008.872628013
3058
11ANT03LI1850Flagellin/FlaB131470.847.90961415
11BNT03LI3663LipL3636949.314.475113610
1184
1595
1163
953
12NT03LI3555LipL4137384.655.737876341
55817
13NT03LI3555LipL4137384.655.737892161
75025
14NT03LI2251Putative lipoprotein44713.716.900496547
51114
15NT03LI2251Putative lipoprotein44713.716.9004105465
67521
16NT03LI1213LipL7162027.265.33561102
17NT03LI0139Conserved hypothetical protein61989.425.52942216
18NT03LI0139Conserved hypothetical protein61989.425.529455218
19NT03LI2676Carboxy-terminal processing protease precursor66174.277.09591343
20NT03LI2676Carboxy-terminal processing protease precursor66174.277.09593119
21NT03LI2676Carboxy-terminal processing protease precursor66174.277.095965715
22NT03LI0330Penicillin G acylase precursor95417.058.6793782
23NT03LI4002Putative lipoprotein46340.419.78522269
24NT03LIA0039Conserved hypothetical protein49905.838.969660039
2579
25NT03LI3081Outer membrane efflux protein59860.329.50552126
26NT03LI3081Outer membrane efflux protein59860.329.50553549
27NT03LI3222Outer membrane efflux protein63512.259.5550703
28NT03LI3222Outer membrane efflux protein63512.259.55501434
29NT03LI0545LigA128128.556.8598133253
2177
2316
80721
  • a Proteins of TX-114-extracted IVCL identified by mass spectrometry. Spot numbers correspond to those shown in Fig. 1. Locus, annotation, mass, and isoelectric point information is as provided by The Institute for Genomic Research based on the annotation of L. interrogans Copenhageni Fiocruz L1-130 (http://www.tigr.org/tigr-scripts/CMR2/GenomePage3.spl?database=ntli03). A Mascot search score of mass spectrometry results is also provided, as is the number of peptides used to provide the score.