TABLE 3.

Intracellularly expressed genes of L. monocytogenes EGD-e controlled by different regulatorsa

IV geneFCIC gene (4 h)FCIC gene (8 h)FCGene designationDescription of product
RpoN-regulated genes
    lmo0048−3.05lmo0048−7.02lmo0048−4.18Similar to Staphylococcus two-component sensor histidine kinase AgrB
lmo0049−3.18lmo0049−3.01lmo0049 protein
lmo01222.00Similar to phage proteins
    lmo01362.20lmo01362.53Similar to oligopeptide ABC transporter; permease protein
    lmo01525.56lmo01522.67Similar to oligopeptide ABC transporter-binding protein
    lmo0210−2.14 ldh Similar to l-lactate dehydrogenase
lmo05203.09lmo05202.75Similar to transcription regulator
lmo06462.73lmo06463.29Similar to unknown proteins
    lmo09722.66 dltC d-Alanyl carrier protein
    lmo09744.95 dltA d-Alanine-d-alanyl carrier protein ligase
    lmo1053−4.03 pdhB Highly similar to pyruvate dehydrogenase, E1 beta subunit
    lmo1055−2.74 pdhD Highly similar to E3 subunit of pyruvate dehydrogenase complex
    lmo12932.25lmo12933.88lmo12933.29 glpD Similar to glycerol-3-phosphate dehydrogenase
−2.93lmo1388−4.13lmo1388−3.17 tcsA T-cell-stimulating antigen; lipoprotein
    lmo1407−3.25 pflC Pyruvate-formate lyase-activating enzyme
    lmo15382.20lmo15383.11lmo15382.46 glpK Similar to glycerol kinase
    lmo1934−6.46lmo1934−9.33lmo1934−5.84 hup Similar to nonspecific DNA binding protein Hup
    lmo2032−2.53lmo2032−3.99lmo2032−2.60 ftsZ Highly similar to cell division initiation protein FtsZ
    lmo21145.45lmo21143.94lmo21144.13Similar to ABC transporter-ATP binding protein
    lmo21155.87lmo21152.99lmo21153.33Similar to ABC transporter; permease
    lmo24683.34lmo24682.55lmo24682.26 clpP ATP-dependent Clp protease, proteolytic subunit
lmo2478−2.63 trxB Thioredoxin reductase
    lmo26172.51lmo26175.22lmo26175.22 rplF Ribosomal protein L6
lmo28292.82lmo28292.62Similar to yeast protein Frm2p involved in fatty acid signaling
SigB-regulated genes
lmo0013−2.12 qoxA AA3-600 quinol oxidase subunit II
lmo04052.17Similar to phosphate transport proteins
    lmo05932.21lmo05933.17lmo05932.72Similar to transport proteins
    lmo08802.39lmo08802.52lmo08802.39Similar to wall-associated protein precursor; has LPXTG motif
    lmo09112.35lmo09115.11lmo09114.60lmo0911 protein
lmo09562.84lmo09562.84Similar to N-acetylglucosamine-6P-phosphate deacetylase
lmo14212.70lmo14212.35 opuBA Similar to glycine betaine-carnitine-choline ABC transporter; ATP-binding protein
    lmo14332.00lmo14332.49lmo14332.21Similar to glutathione reductase
    lmo15394.20lmo15397.09lmo15395.45Similar to glycerol uptake facilitator
    lmo18834.80lmo18833.82lmo18832.99Similar to chitinases
    lmo20852.97lmo20856.16lmo20854.41Putative peptidoglycan-bound protein with LPXTG motif
    lmo22054.38lmo22053.79lmo22054.41 gpmA Similar to phosphoglyceromutase 1
    lmo22698.39lmo22697.48lmo22695.59lmo2269 protein
    lmo24852.41lmo24852.06Similar to B. subtilis YvlC protein
PrfA-regulated genes
lmo00192.83lmo00192.59lmo0019 protein
lmo01692.53lmo01692.05Putative sugar uptake protein
    lmo02003.45lmo02004.48lmo02004.66 prfA Listeriolysin positive regulatory factor A
lmo02018.08lmo02018.97 plcA Phosphatidylinositol-specific phospholipase C
lmo02023.66lmo02025.57 hly Listeriolysin O
    lmo020310.82lmo020318.76lmo020319.24 mpl Zinc metalloproteinase precursor
    lmo02047.33lmo02049.40lmo020413.96 actA Actin assembly-inducing protein
    lmo02059.12lmo020512.97lmo020517.63 plcB Phospholipase C
    lmo02782.41lmo02783.30lmo02782.52Similar to sugar ABC transporter; ATP-binding protein
    lmo04392.36lmo04392.63lmo04392.74Weakly similar to a module of peptide synthetase
    lmo06022.24lmo06023.01lmo06022.29Weakly similar to transcription regulator
    lmo0641−3.22Zinc uptake P-type ATPase
    lmo06542.72lmo06543.29lmo06542.68lmo0654 protein
lmo06762.61lmo06762.06Similar to flagellar biosynthesic protein FliL
lmo07812.29lmo07812.25Similar to mannose-specific PTS system component IID
lmo07822.42lmo07822.24Similar to mannose-specific PTS system component IIC
lmo07832.96lmo07832.67Similar to mannose-specific PTS system component IIB
lmo07962.93Conserved hypothetical protein
    lmo08384.58lmo08389.30lmo08389.22 uhpT Highly similar to hexose phosphate transport protein
lmo09132.52lmo09133.29Similar to succinate semialdehyde dehydrogenase
    lmo09532.13lmo09532.59lmo09532.27lmo0953 protein
lmo22132.69lmo22132.28Similar to unknown protein
lmo22192.45 prsA Similar to posttranslocation molecular chaperone
    lmo23912.87Conserved hypothetical protein similar to B. subtilis YhfK protein
lmo2477−2.22 galE UDP-glucose 4-epimerase
    lmo25712.46Similar to nicotinamidase
    lmo25722.61Similar to chain A, dihydrofolate reductase
    lmo25732.71Similar to zinc binding dehydrogenase
lmo26962.70Similar to hypothetical dihydroxyacetone kinase
lmo26972.44lmo2697 protein
lmo27482.16Similar to B. subtilis stress protein YdaG
PrfA- and SigB-regulated genes
lmo04332.84 inlA Internalin A
lmo04342.05lmo04342.06 inlB Internalin B
    lmo07942.68lmo07943.64lmo07943.37Similar to B. subtilis YwnB protein
lmo09943.44lmo09942.94lmo0994 protein
    lmo16942.40lmo16942.79lmo16942.58Similar to CDP-abequose synthase
    lmo17862.88lmo17867.20lmo17869.55 inlC Internalin C
lmo21572.73lmo21572.37 sepA SepA
lmo22303.65lmo22303.68Similar to arsenate reductase
    lmo25702.47lmo2570 protein
    lmo26732.24Conserved hypothetical protein
lmo26952.58Similar to dihydroxyacetone kinase
PrfA-, SigB-, and RpoN-regulated genes
lmo16022.34lmo16022.24Similar to unknown proteins
CtsR-regulated genes
    lmo02292.60 ctsR Highly similar to transcription repressor of class III stress genes
    lmo02304.21lmo02303.03lmo02302.40Similar to B. subtilis YacH protein
    lmo02314.55lmo02313.76lmo02313.10Similar to arginine kinase
    lmo02322.83lmo02322.61lmo02322.62 clpC Endopeptidase Clp ATP-binding chain C
    lmo099738.80lmo099754.22lmo099742.40 clpE ATP-dependent protease
    lmo22068.57lmo22065.74lmo22066.04 clpB Similar to endopeptidase Clp, ATP binding chain B
    lmo24683.34lmo24682.55lmo24682.26 clpP ATP-dependent Clp protease, proteolytic subunit
HrcA-regulated genes
    lmo14723.34lmo14722.62lmo14722.75 dnaJ Heat shock protein DnaJ
    lmo14733.23 dnaK Class I heat shock protein; molecular chaperone DnaK
    lmo14743.84lmo14742.77lmo14742.77 grpE Heat shock protein GrpE
    lmo14754.23lmo14752.98lmo14752.96 hrcA Transcription repressor of class I heat shock gene hrcA
    lmo20683.01 groEL Class I heat shock protein; chaperone GroEL
    lmo20692.82 groES Class I heat shock protein; chaperone GroES
OhrR-regulated genes
    lmo21994.93lmo21994.75lmo21993.25 ohrA Similar to OhrA
    lmo22003.81lmo22003.77lmo22002.25 ohrR Similar to transcription regulator OhrR
  • a IV, intravacuolar; IC, intracytosolic; FC, fold change.