TABLE 2.

Species- and strain-specific genes of L. monocytogenes EGD-e regulated intracellularlya

IV geneFCIC gene (4 h)FCIC gene (8 h)FCGene designationDescription of product
Species-specific genes
    lmo02003.45lmo02004.48lmo02004.66 prfA Listeriolysin-positive regulatory factor A
lmo02018.08lmo02018.97 plcA Phosphatidylinositol-specific phospholipase C
lmo02023.66lmo02025.57 hly Listeriolysin O
    lmo020310.82lmo020318.76lmo020319.24 mpl Zinc metalloproteinase precursor
    lmo02047.33lmo02049.40lmo020413.96 actA Actin assembly-inducing protein precursor
    lmo02059.12lmo020512.97lmo020517.63 plcB Phospholipase C
lmo02062.84lmo02063.12lmo0206 protein
    lmo02573.06lmo02572.66lmo02572.15Similar to unknown protein
    lmo0419−3.44lmo04194.16Similar to unknown protein
lmo04332.84 inlA Internalin A
lmo04342.05lmo04342.06 inlB Internalin B
    lmo04452.04lmo04453.30lmo04452.62Similar to transcription regulator
lmo074812.44lmo07487.25lmo0748 protein
    lmo07503.31lmo075030.42lmo075023.29lmo0750 protein
    lmo07512.55lmo075125.84lmo075116.02lmo0751 protein
    lmo07522.79lmo075232.47lmo075221.24Weakly similar to a putative haloacetate dehalogenase
lmo07533.56lmo07532.58Similar to transcription regulator, Crp/Fnr family
lmo07544.48lmo07543.63Weakly similar to a bile acid 7-alpha dehydratase
    lmo08384.58lmo08389.30lmo08389.22 uhpT Highly similar to hexose phosphate transport protein
lmo10812.11Similar to glucose-1-phosphate thymidyl transferase
lmo1082−3.70lmo1082−2.49Similar to dTDP-sugar epimerase
    lmo1083−2.48lmo1083−2.59Similar to dTDP-d-glucose 4,6-dehydratase
    lmo1084−2.09lmo1084−2.10Similar to dTDP-l-rhamnose synthetase
    lmo11007.57 cadA Cadmium resistance protein
    lmo17862.88lmo17867.20lmo17869.55 inlC Internalin C
lmo1876−2.32
lmo1877−2.35lmo1877−2.03Similar to formyl-tetrahydrofolate synthetase, N-terminal part
lmo19684.55lmo19683.68Similar to creatinine amidohydrolases
lmo19694.33lmo19693.74Similar to 2-keto-3-deoxygluconate-6-phosphate aldolase
lmo19703.55lmo19702.74Similar to putative phosphotriesterase-related proteins
lmo19713.12lmo19713.01Similar to pentitol PTS system enzyme IIC component
lmo19736.16lmo19736.32Similar to PTS system enzyme IIA component
lmo197410.53lmo19748.78Similar to transcription regulators, GntR family
    lmo20852.97lmo20856.16lmo20854.41Putative peptidoglycan-bound protein with LPXTG motif
lmo21572.73lmo21572.37 sepA SepA
lmo22262.86Similar to unknown proteins
    lmo22572.13lmo22572.25lmo22572.43Hypothetical protein
lmo22705.15lmo22702.91 comK Similar to competence transcription factor ComK, N terminal part
lmo26722.80Weakly similar to transcription regulator
lmo27732.13Similar to transcription antiterminator
lmo28269.06lmo28266.08Similar to efflux proteins
Strain-specific genes
    lmo03982.55Similar to PTS system enzyme IIA
    lmo03992.84lmo03992.46Similar to fructose-specific PTS enzyme IIB
    lmo04002.42Similar to fructose-specific PTS enzyme IIC
lmo04342.05lmo04342.06 inlB Internalin B
    lmo04452.04lmo04453.30lmo04452.62Similar to transcription regulator
lmo074812.44lmo07487.25lmo0748 protein
    lmo07503.31lmo075030.42lmo075023.29lmo0750 protein
    lmo07512.55lmo075125.84lmo075116.02lmo0751 protein
lmo07832.96lmo07832.67Similar to mannose-specific PTS system enzyme IIB
lmo0888−2.13lmo0888−2.27Similar to B. subtilis YdcE protein
lmo1081−2.11Similar to glucose-1-phosphate thymidyl transferase
lmo1082−3.70lmo1082−2.49Similar to dTDP-sugar epimerase
    lmo1083−2.48lmo1083−2.59Similar to dTDP-d-glucose 4,6-dehydratase
    lmo1084−2.09lmo1084−2.10Similar to dTDP-l-rhamnose synthetase
    lmo11007.57 cadA Cadmium resistance protein
lmo1876−2.32
lmo1877−2.35lmo1877−2.03Similar to formyl-tetrahydrofolate synthetase, N-terminal part
lmo19684.55lmo19683.68Similar to creatinine amidohydrolases
lmo19694.33lmo19693.74Similar to 2-keto-3-deoxygluconate-6-phosphate aldolase
lmo19703.55lmo19702.74Similar to putative phosphotriesterase-related proteins
lmo19713.12lmo19713.01Similar to pentitol PTS system enzyme IIC component
lmo19736.16lmo19736.32Similar to PTS system enzyme IIA component
lmo197410.53lmo19748.78Similar to transcription regulators, GntR family
    lmo22698.39lmo22697.48lmo22695.59lmo2269 protein
lmo22705.15lmo22702.91 comK Similar to competence transcription factor ComK, N terminal part
  • a Species- and strain-specific genes of EGD-e were identified by comparing the genome sequences of L. monocytogenes EGD-e, L. innocua, and L. monocytogenes F2365 serotype 4b by using an identity of >50% and >75% coverage of the protein sequences. Fold change (FC) indicates the ratio of expression intensities of the intravacuolar (IV) and intracytosolic (IC) L. monocytogenes genes to those of genes of in vitro-growing L. monocytogenes.