TABLE 1.

Predicted meningococcal OM proteins identified in this studya

Category or protein namebNCBI locus tag (NMB no.)No. of peptides in:Molecular mass (kDa)cpIcPredicted function (COGS)d
OMOMVLPS OM
PorA outer membrane protein142916261042.19.3Cell wall/membrane biogenesis
PorB outer membrane proteine203914201335.77.8Cell wall/membrane biogenesis
Opc proteinf1053916730.010.0Not in COGS
PilQ protein1812810482.49.8Intracellular trafficking and secretion
Macrophage infectivity potentiator156752928.95.8Posttranslational modification, protein turnover, and chaperones
Rmp proteine038247226.27.6Cell wall/membrane biogenesis
Cell binding factor, putative034543331.49.5Posttranslational modification, protein turnover, and chaperones
Outer membrane protein Omp850182310588.48.9Cell wall/membrane biogenesis
Multidrug efflux pump channel protein171427150.48.6Cell wall/membrane biogenesis
Outer membrane protein P1, putative008823150.59.6Lipid transport and metabolism
Adhesion and penetration protein1985221159.99.4Cell wall/membrane biogenesis
Organic solvent tolerance protein, putative028013084.28.9Cell wall/membrane biogenesis
Outer membrane protein NspAg066312018.49.8Cell wall/membrane biogenesis
VacJ-related protein196110029.54.8Cell wall/membrane biogenesis
TbpBh046010077.46.0Not in COGS
TbpAh0461031102.09.6Inorganic ion transport and metabolism
Maltose phosphorylase039002285.45.5Carbohydrate transport and metabolism
TonB-dependent receptor1497020104.29.6Inorganic ion transport and metabolism
Conserved hypothetical protein213402069.88.6Cell wall/membrane biogenesis
Phospholipase A1 putative046401042.98.9Cell wall/membrane biogenesis
Conserved hypothetical protein133301065.710.3Cell cycle control, mitosis, and meiosis
Lactoferrin binding protein A1540010105.69.6Inorganic ion transport and metabolism
Serine type peptidase1998010157.69.2Cell wall/membrane biogenesis
Conserved hypothetical protein205001066.65.5Unknown
Cardiolipin synthetase family protein143401057.39.3Lipid transport and metabolism
Phosphoribosylformylglycinamidine synthase1996001143.85.4Nucleotide transport and metabolism
  • a Proteins predicted by the PSORTb algorithm (located at http://psort.org/) to have an outer membrane location which were detected in the OM preparations.

  • b The protein name is the name in the annotation of the MC58 genome located at http://www.tigr.org/ unless indicated otherwise.

  • c The theoretical molecular mass and pI values were calculated using the ProteinLynx Global Server Package (version 2.05).

  • d Predicted functional categories were derived from the Clusters of Orthologous Groups of proteins (COGS) database (located at http://www.ncbi.nlm.nih.gov/sutils/). Similar results were obtained in an analysis of a second independent preparation of each fraction.

  • e The name in the annotation of the MC58 genome located at http://www.tigr.org/ differs from the previously published name used in the text. See reference 20.

  • f The name in the annotation of the MC58 genome located at http://www.tigr.org/ differs from the previously published name used in the text. See reference 29.

  • g See reference 24.

  • h The name in the annotation of the MC58 genome located at http://www.tigr.org/ differs from the previously published name used in the text. See reference 42.