Experimental designa

Treatment groupWk 0Wk 4Wk 13Wk 17
1F. hepaticaSCITT
2F. hepaticaBCGSCITT
3BCGF. hepaticaSCITT
  • a Calves were allocated to one of four treatment groups, as shown. In the case of groups 2, 3, and 4, the SCITT was carried out 13 weeks post BCG immunization. In addition to the groups shown above, two additional calves, from the same farm as the other animals and within the same age range, were used as donors of blood monocytes for macrophage preparation and in vitro stimulation with PPD-B and F. hepatica ES products.