TABLE 1.

Transposon insertion sites identified in this studya

MutantDisrupted geneProductPutative functionInsertion distance from start codon (bp)b
7A6wcbAPutative capsular polysaccharide export proteinCapsular polysaccharide biosynthesis1,301
13E8wcbAPutative capsular polysaccharide export proteinCapsular polysaccharide biosynthesis713
13H8wcbBPutative capsular polysaccharide glycosyltransferase biosynthesis proteinCapsular polysaccharide biosynthesis46
1B9wcbCPutative capsular polysaccharide export proteinCapsular polysaccharide biosynthesis1,035
8F1wcbCPutative capsular polysaccharide export proteinCapsular polysaccharide biosynthesis1,018
6E3wcbCPutative capsular polysaccharide export proteinCapsular polysaccharide biosynthesis518
4A4wcbDPutative ABC transporter transmembrane proteinCapsular polysaccharide biosynthesis961
JCX2C5wzmPutative capsule polysaccharide export ABC transporter transmembrane proteinCapsular polysaccharide biosynthesis466
8C3wcbEPutative glycosyltransferaseCapsular polysaccharide biosynthesis196
13G10wcbEPutative glycosyltransferaseCapsular polysaccharide biosynthesis945
4H9wcbEPutative glycosyltransferaseCapsular polysaccharide biosynthesis1,464
5E12wcbEPutative glycosyltransferaseCapsular polysaccharide biosynthesis1,346
6G8wcbHPutative glycosyltransferaseCapsular polysaccharide biosynthesis625
8H1wcbHPutative glycosyltransferaseCapsular polysaccharide biosynthesis731
9G8wcbHPutative glycosyltransferaseCapsular polysaccharide biosynthesis474
JCX2B10wcbHPutative glycosyltransferaseCapsular polysaccharide biosynthesis75
4E6wcbIPutative capsular polysaccharide biosynthesis proteinCapsular polysaccharide biosynthesis459
JCX2B2wcbJPutative capsular polysaccharide biosynthesis proteinCapsular polysaccharide biosynthesis516
1A6wcbNPutative d-glycero-d-manno-heptose 1,7-bisphosphate phosphataseCapsular polysaccharide biosynthesis258
13G3wcbQPutative capsular polysaccharide transmembrane export proteinCapsular polysaccharide biosynthesis711
12F5wcbRPutative type I polyketide synthaseCapsular polysaccharide biosynthesis761
JCX2A9wcbRPutative type I polyketide synthaseCapsular polysaccharide biosynthesis451
8A8BPSL0776RecAHomologous recombination, SOS response207
7G7BPSL2825Putative PabBBiosynthesis of para-aminobenzoate1,352
9D6serCPhosphoserine aminotransferaseSerine biosynthesis360
6H5trpGAnthranilate synthase component IITryptophan biosynthesis447
12C6leuBIsopropylmalate dehydrogenaseLeucine biosynthesis769
8H10leuBIsopropylmalate dehydrogenaseLeucine biosynthesis899
13B11aroBDehydroquinate synthaseChorismate biosynthesis532
7A1BPSL3147Putative lipoprotein39.16% amino acid identity to Shigella flexneri VacJ lipoprotein618
6H4BPSL1039Putative ABC transporterPutative transmembrane ABC transporter1,293
7G1BPSL0634Putative oxidoreductaseEnergy production and conversion2,306
6A8BPSL0634Putative oxidoreductaseEnergy production and conversion3,191
13D4ppsAPutative phosphoenol pyruvate synthaseCarbohydrate transport and metabolism704
JCX2D8Upstream of ihfAPutative integration host factor alpha subunit IhfADNA replication, recombination, and repairNA
9F5nthPutative endonuclease IIIDNA replication, recombination, and repair150
7D6nthPutative endonuclease IIIDNA replication, recombination, and repair134
JCX2E12BPSL0175/BPSL1060Putative hypothetical bacteriophage proteinUnknown149
JCXD3BPSL0175/BPSL1060Putative hypothetical bacteriophage proteinUnknown123
  • a Insertion sites within the capsule region are indicated in Fig. 4b.

  • b NA, not applicable.