TABLE 1.

Oligonucleotide primers used in this study

PrimerSequence (5′-3′)a
F1-lctPACGCGTCGACAAAGGATTCGTTATGGCACT
R1-lctPACGCGTCGACTACTACGCCTGAATGCAAAC
F2-lctPGCGTCTTACCAAACGCTGTA
R2-lctPGGAGAAGAACGCACCGATC
RCATTTTACGATGCGATTGGGATATA
  • a SalI recognition sites are underlined.