TABLE 1.

PCR primer sequences and predicted product lengths

Target mRNA5′ Primer3′ PrimerProduct size (bp)
TLR25′-GATTGTGGACAACATCATTGACTC-3′5′-AGAGCTGCTTTCAAGTTTTCCC-3′294
TLR45′-AGTCTGAAATTGCTGAGCTCAAAT-3′5′-GCGACGTTAAGCCATGGAAG-3′190
TLR155′-GTTCTCTCTCCCAGTTTTGTAAATAGC-3′5′-GTGGTTCATTGGTTGTTTTTAGGAC-3′262
IL-1β5′-CGCTCACAGTCCTTCGACATC-3′5′-CCGCTCATCACACACGACATGT-3′230
18S RNA5′-GCTGAAACTTAAAGGAATTGACG-3′5′-CTGTTATTGCTCAATCTCGGG-3′304