TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype or descriptionReference or source
Strain
    M. catarrhalis
        7169Wild-type disease isolate, serum resistant11
        7169.1uspA1 mutant of 7169, serum resistant, kanamycin resistantThis study
        7169.2uspA2 mutant of 7169, serum sensitive, spectinomycin resistantThis study
        7169Δ2uspA2 deletion mutant of 7169, serum sensitive, spectinomycin resistantThis study
        ETSU5Wild-type disease isolate, serum resistantSteven Berk
        ETSU5.1uspA1 mutant of ETSU5, serum resistant, kanamycin resistantThis study
        ETSU5.2uspA2 mutant of ETSU5, serum sensitive, spectinomycin resistantThis study
        ETSU22Wild-type disease isolate, serum resistantSteven Berk
        ETSU22.1uspA1 mutant of ETSU22, serum resistant, kanamycin resistantThis study
        ETSU22.2uspA2 mutant of ETSU22, serum sensitive, spectinomycin resistantThis study
        ETSU26Wild-type disease isolate, serum resistantSteven Berk
        ETSU26.1uspA1 mutant of ETSU26, serum resistant, kanamycin resistantThis study
        ETSU26.2uspA2 mutant of ETSU26, serum sensitive, spectinomycin resistantThis study
        FIN2344Wild-type isolate, serum resistantMerja Helminen
        FIN2344.1uspA1 mutant of FIN2344, serum resistant, kanamycin resistantThis study
        FIN2344.2uspA2 mutant of FIN2344, serum sensitive, spectinomycin resistantThis study
        FIN2344Δ2uspA2 deletion mutant of FIN2344, serum sensitive, spectinomycin resistantThis study
        FIN2406Wild-type isolate, serum resistantMerja Helminen
        FIN2406.1uspA1 mutant of FIN2406, serum resistant, kanamycin resistantThis study
        FIN2406.2uspA2 mutant of FIN2406, serum sensitive, spectinomycin resistantThis study
        O12EWild-type disease isolate, serum resistant1
        O12E.1uspA1 mutant of O12E, serum resistant, kanamycin resistant30
        O12E.2uspA2 mutant of O12E, serum sensitive, spectinomycin resistantThis study
        O12EΔ2uspA2 deletion mutant of O12E, serum sensitive, spectinomycin resistantThis study
        O35EWild-type disease isolate, serum resistant3
        O35E.1uspA1 mutant of O35E, serum resistant, kanamycin resistant2
        O35E.2uspA2 mutant of O35E, serum sensitive, spectinomycin resistantThis study
        O35EΔ2uspA2 deletion mutant of O35E, serum sensitive, spectinomycin resistant6
        V1118Wild-type isolate, serum resistantFrederick Henderson
        V1118.1uspA1 mutant of V1118, serum resistant, kanamycin resistantThis study
        V1118.2uspA2 mutant of V1118, serum sensitive, spectinomycin resistantThis study
        V1120Wild-type isolate, serum resistantFrederick Henderson
        V1120.1uspA1 mutant of V1120, serum resistant, kanamycin resistantThis study
        V1120.2uspA2 mutant of V1120, serum sensitive, spectinomycin resistantThis study
        V1145Wild-type isolate, serum resistantFrederick Henderson
        V1145.1uspA1 mutant of V1145, serum resistant, kanamycin resistantThis study
        V1145.2uspA2 mutant of V1145, serum sensitive, spectinomycin resistantThis study
        V1156Wild-type isolate, serum resistantFrederick Henderson
        V1156.1uspA1 mutant of V1156, serum resistant, kanamycin resistantThis study
        V1156.2uspA2 mutant of V1156, serum sensitive, spectinomycin resistantThis study
    N. gonorrhoeae
        FA19Wild-type strain, serum resistant, binds C4BP37
        UU1Wild-type strain, serum sensitive, does not bind C4BP61
    H. influenzae
        DB117Host strain for cloning experiments54
Plasmids
    pAA2pCR2.1 containing a spectinomycin resistance cartridge inserted between the two flanking regions of the uspA2 gene from M. catarrhalis O12E6
    pUSPA1KANpBluescript containing a mutated uspA1 gene from M. catarrhalis O35E2
    pELU2P44SPECpBS containing an incomplete uspA2 gene from M. catarrhalis P44 into which a spectinomycin resistance cartridge was inserted30
    pGJB103MCloning vector6
    pAA-P2-propGJB103M with the ompP2 promoter cloned into the SphI-AvrII sitesThis study
    pAAO35EU2-P2pGJB103M with the M. catarrhalis O35E uspA2 gene inserted behind the ompP2 promoter in the SphI-AvrII sitesThis study
    pAAO12EU2-P2pGJB103M with the M. catarrhalis O12E uspA2 gene inserted behind the ompP2 promoter in the SphI-AvrII sitesThis study
    pAAFIN2344U2-P2pGJB103M with the M. catarrhalis FIN2344 uspA2 gene inserted behind the ompP2 promoter in the SphI-AvrII sitesThis study
    pAA7169U2-P2pGJB103M with the M. catarrhalis 7169 uspA2 gene inserted behind the ompP2 promoter in the SphI-AvrII sitesThis study