TABLE 2.

P1 adsorption and P2 sensitivity of EHEC strains

Strain% Adsorbed P1a% Survival after P2 challengeb
EDL933 (wild type)27.438
TEA007 (galE::pTHE001)93.50.054
TEA023 (ΔgalU::aad-7)96.60.29
TEA026 (ΔgalETKM::aad-7)95.20.014
TEA028 (ΔgalETKM::tetA)98.40.35
TEA040 (Gal+ revertant)15.632
  • a The percentage of adsorbed P1 was determined as follows: (number of P1 plaques obtained after incubation with bacteria/number of P1 plaques obtained after incubation without bacteria) × 100%. Each experiment was performed in triplicate, and the data are means. The P value for a comparison of each of the gal mutants and the wild type was less than 0.006. The P value for a comparison of TEA040 and the wild type was 0.65.

  • b The percentage of survival after P2 challenge was determined as follows: (number of bacteria after incubation with P2/number of bacteria after incubation without P2) × 100%. Each experiment was performed in triplicate, and the data are means. The P value for a comparison of each of the gal mutants and the wild type was less than 0.03. The P value for a comparison of TEA040 and the wild type was 0.10.